Protein

Protein accession
A0AAF0D8F8 [UniProt]
Representative
3NmsY
Source
UniProt (cluster: phalp2_38947)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MGHVFNRPARPVERVFVHCSASDVPAHDDVKVIQQWHKQRWPDLCATEAGYHFFIKKDGTIQPSRSLEKKPVAQAGHNSHTIAICLHGLKVAKFTEEQKTALQSLCQEIFDAYDGNITFHGHREVAAKDCPVIDYKAWLGLDRMGRLHGRSPTKPVATPMPTLKVGSRGGMVMELQSLLGFTGSQVDGKFGGATERAVKAFQRSAGLTDDGIVGRNTWKALQTKGGA
Physico‐chemical
properties
protein length:227 AA
molecular weight:24913,2 Da
isoelectric point:9,34
hydropathy:-0,39
Representative Protein Details
Accession
3NmsY
Protein name
3NmsY
Sequence length
215 AA
Molecular weight
23733,66950 Da
Isoelectric point
8,31889
Sequence
MRAIFSSLLAFLRQLFNTGKASAPQEQEQEKVIAPPIFTETKESIKPIIEHTTASVQKVLIVNNQPYTFAAPTTRDVSRVFIHCTASSNPKYDLASVKRDHTNLGYKRDDGVLDVAYHFFITFAGELQLGRKLWSNPDAQKGHNTGTIAICLQGLVVKDFTSAQMETLTALCQQINEAYGGSVTFHGHNEVSNRGCPVFDYRAALKLDSKGNLGV
Other Proteins in cluster: phalp2_38947
Total (incl. this protein): 2 Avg length: 221,0 Avg pI: 8,83
Protein ID Length (AA) pI
3NmsY 215 8,31889
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_2920
NQqI
 114 55,4% 148 5.201E-58
2 phalp2_30941
13o5z
 7 51,3% 146 7.275E-46
3 phalp2_3887
6RreM
 7260 32,4% 151 1.373E-21
4 phalp2_28564
7Z48s
 97 29,1% 137 4.116E-20
5 phalp2_28171
8yYQh
 287 29,6% 145 9.061E-17
6 phalp2_11894
2jrH4
 329 28,5% 147 2.636E-15
7 phalp2_12381
8yxE3
 11 27,3% 139 5.584E-14
8 phalp2_36193
72BPm
 409 26,0% 138 3.470E-13
9 phalp2_23565
7vNZv
 154 30,1% 146 6.372E-13
10 phalp2_39380
4H4oF
 116 27,0% 148 9.761E-12

Domains

Domains [InterPro]
Disordered region
Ami2
Representative sequence (used for alignment): 3NmsY (215 AA)
Member sequence: A0AAF0D8F8 (227 AA)
1 215 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Aeromonas phage HJ05
[NCBI] 		3035942 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OQ594355 [NCBI]
CDS location
range 49305 -> 49988
strand +
CDS
ATGGGTCACGTATTCAACCGGCCTGCTCGGCCTGTCGAGCGTGTCTTCGTCCACTGTTCAGCCTCTGATGTACCGGCGCATGATGATGTGAAGGTTATCCAGCAATGGCACAAGCAACGCTGGCCCGACCTTTGCGCCACCGAGGCCGGTTACCACTTTTTCATCAAGAAGGATGGGACAATCCAGCCCAGCCGTAGCTTGGAGAAAAAGCCGGTAGCTCAGGCGGGACACAACTCTCACACCATCGCAATTTGCTTGCATGGGTTGAAGGTAGCGAAGTTCACAGAAGAACAAAAGACGGCGTTGCAGAGTCTCTGCCAAGAGATTTTCGATGCCTATGACGGGAACATCACTTTCCACGGCCATCGAGAGGTGGCCGCAAAAGATTGCCCAGTAATCGACTACAAGGCTTGGCTCGGTCTTGACCGGATGGGTCGCCTACACGGGCGCTCACCTACCAAGCCGGTAGCTACTCCGATGCCTACCTTGAAAGTCGGAAGCCGTGGCGGTATGGTGATGGAGTTGCAGTCCCTTCTGGGCTTTACCGGCTCGCAAGTGGATGGCAAGTTTGGTGGAGCTACCGAGCGGGCCGTCAAGGCGTTCCAGCGCAGTGCTGGGCTGACTGATGACGGCATCGTAGGTCGCAACACTTGGAAGGCTCTACAGACCAAAGGAGGTGCGTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (3NmsY) rather than this protein.
PDB ID
3NmsY
Method AlphaFoldv2
Resolution 82.40
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50