Protein

Protein accession
A0A8S5QBA5 [UniProt]
Representative
8LrgT
Source
UniProt (cluster: phalp2_7901)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAYRFLTQYDALRFTPNALVQSVFGFPRVITNITIHWWGRPEWKQKFDEVIRFFCELNSTQTSAHEVISDGVVACLVDHANAAWANGNSKGNAQSITLECNPRMSAGDFETVCERVADIWIMHGQILPVTEHRDWFATECCGTYRKGEVAARALQIYEAKKGGKTAITKVAEKATQPKGKDDKSMADAISELRDSWAPGIEHVRHHGANWMALQNVSRQTQELKDTWTPGIPNVKFEGSAYKLLRENLEAQRETNALLKQLIAAQTSNKVGE
Physico‐chemical
properties
protein length:272 AA
molecular weight:30565,2 Da
isoelectric point:7,08
hydropathy:-0,44
Representative Protein Details
Accession
8LrgT
Protein name
8LrgT
Sequence length
214 AA
Molecular weight
23035,50930 Da
Isoelectric point
6,36610
Sequence
MNIIETFTAKGYTPAAYVAGTFGQPRTLDGIVIHHWGAPGQTHDGVVNFFVNGPGTTSAHFVASAGKVHCLVSPDDAAWHSGNAKGNATTIGIECRPEATDADYQTVAELVAWLRDTYNATLPLSTHREWQNTACPGVWDLARIDRLASTATPTTPATPLGDPLAKLNDKQQQELYTWVKEIRENTKDLNVDSGTGKLSLRQAIANIRAKVGAK
Other Proteins in cluster: phalp2_7901
Total (incl. this protein): 13 Avg length: 230,8 Avg pI: 6,18
Protein ID Length (AA) pI
8LrgT 214 6,36610
4Lrrt 175 4,85657
4SZR6 175 4,85657
4XSf0 283 5,29213
4YhXc 273 5,18453
8Lrhn 214 6,21917
A0A3G3LY06 214 6,36610
A0A3G3M107 214 6,21917
A0AA96SDY5 215 7,77173
A0AAE8Y861 240 6,70378
A0AAX4LW93 240 6,70378
A0AA50KIU8 272 6,66627
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_31988
59Np4
 130 68,7% 157 1.475E-84
2 phalp2_25595
3X4EC
 26 56,5% 168 1.493E-68
3 phalp2_15294
1dl82
 2 51,8% 160 4.924E-61
4 phalp2_25234
40uRC
 5 48,2% 170 6.177E-57
5 phalp2_31556
4fe7g
 22 42,5% 174 5.632E-53
6 phalp2_16559
7s5Uq
 12 42,4% 179 1.993E-46
7 phalp2_13163
2f2Cg
 24 41,5% 202 1.993E-46
8 phalp2_13682
65xN2
 64 44,1% 154 2.442E-42
9 phalp2_13806
716PV
 1 38,0% 163 9.436E-40
10 phalp2_33401
6T3Pt
 1 35,4% 144 5.266E-22

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 8LrgT (214 AA)
Member sequence: A0A8S5QBA5 (272 AA)
1 214 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Myoviridae sp. ctw4b6
[NCBI] 		2825206 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015628 [NCBI]
CDS location
range 9422 -> 10240
strand +
CDS
ATGGCCTACCGATTTCTTACACAATACGACGCGCTACGGTTCACACCAAACGCGCTAGTCCAGTCCGTTTTCGGGTTCCCCCGTGTAATCACAAACATCACGATTCATTGGTGGGGCCGCCCAGAGTGGAAGCAAAAATTTGATGAAGTAATCAGGTTCTTCTGCGAGCTGAATAGCACGCAGACTAGCGCGCACGAGGTCATTTCTGATGGCGTTGTGGCGTGCCTTGTGGATCACGCTAACGCAGCCTGGGCGAACGGCAACAGCAAGGGTAATGCGCAGTCAATCACCCTGGAATGCAACCCACGCATGTCTGCTGGCGACTTCGAGACCGTGTGTGAGCGAGTCGCCGACATCTGGATCATGCACGGGCAAATCCTGCCCGTCACAGAGCATCGGGACTGGTTCGCAACGGAGTGCTGCGGCACCTACCGCAAGGGCGAGGTTGCGGCCCGCGCCCTGCAAATCTACGAGGCGAAAAAAGGCGGGAAGACCGCTATCACTAAGGTTGCTGAGAAGGCGACCCAACCCAAGGGAAAGGACGATAAGAGCATGGCTGACGCTATTAGCGAGCTGCGGGACAGCTGGGCACCCGGTATCGAGCATGTGCGCCATCACGGCGCTAACTGGATGGCGCTGCAGAACGTGAGCCGCCAGACTCAGGAGCTGAAGGACACCTGGACGCCGGGCATCCCGAACGTAAAGTTTGAGGGTTCCGCATATAAGCTGCTCCGTGAGAATTTGGAGGCGCAGCGTGAGACTAACGCGCTGCTGAAGCAGCTTATCGCAGCCCAGACTAGCAACAAGGTAGGAGAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8LrgT) rather than this protein.
PDB ID
8LrgT
Method AlphaFoldv2
Resolution 84.04
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50