Protein

Protein accession
A0A8S5PP24 [UniProt]
Representative
2nYlj
Source
UniProt (cluster: phalp2_39111)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MNLHTVFATKNYCYTDLPNMSKVLGIMVHSTGCNNPNLRRYVGPDDGLLGPTSSANWNQRTINGKVQKLGVHGFIGKLKDGTVATYQVQKWNHKCYHCGVGTSGKSGNSNYIAFEICEDDLTDEAYCKACYREAVELCAYLCEMYKLNPLTNIVCHCEAYKKGFASNHADVMHWWKRFGLSMDQFRVDVKNEMEDNNMTDEKFAELMERYYANQAKKTCPDWAKEEFEEAKQMGITDGSAPMRPATRLETALMTKRAIKSTNK
Physico‐chemical
properties
protein length:263 AA
molecular weight:29871,7 Da
isoelectric point:7,92
hydropathy:-0,59
Representative Protein Details
Accession
2nYlj
Protein name
2nYlj
Sequence length
331 AA
Molecular weight
37124,33310 Da
Isoelectric point
6,45460
Sequence
MRLFESILTLNDCYKAGRTITPKGIMVHSTGANNPWLKRYVPDDGRLGYNQYNNHWNMSGVGACVHGFIGKMADGTVATYQTLPWNWRGWHAASGNKGSANNTHISFEICEDGLTDATYFRAVFNEAAELCAYLCKMYNLNPLTDIICHSEGHTMGIASNHGDVMHWFPKHGENMDTFRQKVYELVNEEFKWNESKVDYIGTVSVKGDVLNIRKQPRVGASIIGKHADGDTVTICAQTDNGWYRVEYPTIGTGYISSAYVSVSDRKAPQSEVKPEVIKDNTPDAWAAEAVNNAVKNGILYGDDTGNYKLHSECTRQEMLIFLYRFYKSIKG
Other Proteins in cluster: phalp2_39111
Total (incl. this protein): 83 Avg length: 339,9 Avg pI: 8,23
Protein ID Length (AA) pI
2nYlj 331 6,45460
1FCY1 317 9,67724
1Jeno 356 9,24697
1jHM1 351 8,72459
1kcEy 351 8,61828
1kryb 351 8,72001
1njCb 351 8,62228
2lDAQ 335 5,89315
3etpH 288 9,23518
3rQA6 351 8,79969
3uIba 351 8,88608
3un4d 304 5,29708
3vG2A 351 8,89743
3vRgv 351 9,01772
3yVw4 350 5,92776
3ygvB 351 8,71956
3ykcd 293 5,11582
5JVHV 351 8,97305
5KvO7 304 5,14281
5L6k9 351 8,80994
5Lc0R 352 8,89169
5LcCu 310 9,00096
5OeTY 351 9,01772
5PKz7 398 9,10257
5PpAb 351 8,80472
5QcjF 351 9,05054
5QdZo 310 8,90465
5Qo8t 304 5,36551
5RNLO 302 5,21011
5TAft 310 8,99336
5TSks 351 9,04364
5Tu5I 351 8,88608
5UDU9 351 8,76385
5WUYW 351 8,89162
5Wadh 351 8,62228
5WrZz 351 8,89162
5Ws0k 351 8,71498
5XctV 398 9,04622
5ZFBY 351 8,61796
61b4t 351 9,06891
63JXF 351 8,97950
6583n 310 8,90465
65dRw 351 8,80981
67Suv 305 5,07535
68TL0 351 8,80472
68eVP 351 8,98968
68l6q 350 9,07478
6azvx 351 9,04377
6c2lA 351 8,88614
6cua1 351 8,79969
6d4J6 351 8,94907
6egE8 351 8,94907
6fT5B 300 5,64402
6iAtJ 356 9,01147
6k3G5 302 5,06296
6kW50 398 9,05202
6kqae 304 5,20511
6mISH 351 8,72439
6mivN 383 9,36927
6mnPY 304 5,28361
6nXHN 351 8,81504
6o0Js 351 8,88602
6o7M1 356 8,96563
6oC22 310 9,00857
6qklX 304 5,13702
6sr8z 351 8,96692
6uynA 351 9,03707
6vzeS 310 9,00083
6wmL7 301 5,58383
7Uj8D 351 8,96692
7oh2H 356 9,38216
84x7I 351 8,88067
86N0Q 351 8,87525
86yiJ 398 9,10869
879jD 351 9,04377
8bzKU 301 5,47475
8eNgn 351 8,92689
8qNFy 285 9,02817
8rnTC 351 8,88067
KBwY 351 8,62672
osnY 361 7,56472
zkJA 351 8,96093
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_17183
3bJIf
 458 61,8% 233 8.862E-99
2 phalp2_36261
3rB1
 176 39,4% 368 1.213E-98
3 phalp2_10292
1gBJ2
 1 38,1% 296 4.259E-72
4 phalp2_32070
5P7Lm
 156 40,0% 252 5.198E-71
5 phalp2_25741
4Lci3
 6 41,6% 228 7.463E-66
6 phalp2_8994
3TJvI
 23 35,9% 292 3.665E-60
7 phalp2_17257
3WAN9
 1 37,2% 271 2.876E-58
8 phalp2_37002
o6En
 47 29,8% 355 5.722E-56
9 phalp2_24341
3TP9g
 1 26,6% 368 5.619E-44
10 phalp2_12285
7dvAF
 4 29,2% 263 1.681E-42

Domains

Domains [InterPro]
Ami2
SH3_3
Unannotated
Representative sequence (used for alignment): 2nYlj (331 AA)
Member sequence: A0A8S5PP24 (263 AA)
1 331 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08239

Taxonomy

  Name Taxonomy ID Lineage
Phage Myoviridae sp. ctwwN25
[NCBI] 		2825209 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015472 [NCBI]
CDS location
range 6735 -> 7526
strand -
CDS
ATGAATCTTCATACTGTATTTGCAACAAAGAATTACTGCTACACTGATTTGCCTAATATGTCTAAAGTTTTGGGCATTATGGTACATTCTACTGGCTGTAATAATCCTAACCTGCGTAGATATGTTGGTCCTGATGATGGATTGCTTGGGCCTACGAGTTCTGCTAACTGGAACCAGAGAACTATCAATGGCAAGGTTCAGAAGCTCGGAGTTCATGGATTCATTGGCAAATTAAAGGACGGCACTGTTGCTACTTATCAGGTTCAGAAGTGGAATCATAAATGCTATCACTGCGGTGTAGGTACTTCTGGTAAGTCTGGTAATAGTAATTACATTGCTTTTGAGATCTGTGAAGATGATCTGACCGATGAGGCATACTGCAAGGCATGCTATAGAGAAGCTGTTGAGCTCTGTGCTTATCTCTGTGAGATGTATAAGCTCAATCCTCTGACTAATATCGTATGTCATTGTGAAGCTTATAAGAAGGGCTTTGCATCTAACCATGCAGATGTAATGCATTGGTGGAAGAGGTTTGGTCTTTCTATGGATCAGTTCCGTGTTGACGTTAAAAATGAAATGGAGGATAATAATATGACTGACGAAAAGTTTGCTGAGCTGATGGAGCGCTATTATGCTAATCAGGCTAAGAAGACCTGCCCTGATTGGGCTAAGGAAGAGTTTGAAGAGGCTAAGCAAATGGGTATTACCGATGGTAGTGCTCCCATGCGGCCTGCTACTCGTCTTGAGACTGCTCTGATGACTAAGCGTGCTATTAAGAGCACTAACAAATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (2nYlj) rather than this protein.
PDB ID
2nYlj
Method AlphaFoldv2
Resolution 88.85
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50