Protein

Protein accession
A0A8S5MGE2 [UniProt]
Representative
NzwP
Source
UniProt (cluster: phalp2_7974)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MVKTIEITNEAKRIANLGIGVDQDGAYGTQCVDLPNYSSVKFFGKALWGNAIDLLSSAAALGYKVEYNEIGNLDSKPRAGAVFVMDTTYIYGHPYGHTGIVIEDSDGYTMKTIEQNVDGNADALYVGGPARYMERNFDGIVGWFYFPVDDNEVVTENSDLISLPEVRVYTVGVDKLNIRNAPSTDAEIIGSYEKGEEFNYMQFCNANGYEWLSYISNSGVRRYVASMNLETFETHGTWRKK
Physico‐chemical
properties
protein length:241 AA
molecular weight:26840,6 Da
isoelectric point:4,63
hydropathy:-0,31
Representative Protein Details
Accession
NzwP
Protein name
NzwP
Sequence length
161 AA
Molecular weight
18325,94120 Da
Isoelectric point
4,39351
Sequence
AVFVMDTTYIYGHPFGHTGVVIEDSDGYTMKTIEQNVDGNADALYVGGPARYMERNFDGIVGWFYYPTDDNEVVEENSDLISLPEVRTYTVGVDKLNIRNAPSTEAEIVGSYEKGEEFNYMEFCNANGYEWLSYISNSGVRRYVASMDLETFETHGTWRKK
Other Proteins in cluster: phalp2_7974
Total (incl. this protein): 4 Avg length: 204,3 Avg pI: 4,34
Protein ID Length (AA) pI
NzwP 161 4,39351
7rCOZ 167 4,20918
A0A4D6B228 248 4,11914
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_5002
1dLD0
 578 62,4% 149 4.536E-68
2 phalp2_23379
5JYK7
 2 35,0% 120 6.278E-17
3 phalp2_18246
7DJw9
 1 27,3% 150 1.343E-04

Domains

Domains [InterPro]
Unannotated
SH3_5
Representative sequence (used for alignment): NzwP (161 AA)
Member sequence: A0A8S5MGE2 (241 AA)
1 161 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF08460

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctHl62
[NCBI] 		2826235 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014899 [NCBI]
CDS location
range 39196 -> 39921
strand +
CDS
ATGGTTAAAACAATTGAAATTACAAATGAAGCAAAAAGAATAGCAAATCTAGGAATAGGTGTTGATCAAGATGGAGCTTATGGAACACAATGTGTAGACTTACCTAACTATTCATCAGTGAAATTTTTTGGTAAAGCATTATGGGGTAATGCTATTGATCTATTAAGTAGTGCTGCTGCATTAGGGTATAAAGTTGAATATAATGAAATAGGAAACTTAGATAGCAAACCACGAGCTGGAGCGGTCTTTGTAATGGATACTACATATATCTATGGACATCCATATGGACATACAGGAATTGTAATTGAAGACAGTGATGGATATACTATGAAAACTATTGAACAAAATGTTGATGGTAATGCAGATGCATTATATGTAGGTGGACCAGCACGTTATATGGAACGTAACTTTGATGGGATTGTTGGTTGGTTCTACTTCCCAGTAGACGATAATGAAGTAGTTACTGAAAACTCTGACTTAATCTCATTGCCTGAAGTACGTGTGTATACAGTTGGAGTTGATAAACTTAATATTAGAAATGCACCATCTACAGATGCAGAAATCATAGGTTCATATGAAAAAGGTGAAGAATTTAATTACATGCAATTCTGTAATGCTAATGGATATGAATGGTTATCTTACATTTCTAATAGTGGTGTAAGACGTTATGTGGCTTCTATGAATCTAGAGACATTTGAAACTCACGGAACATGGAGAAAAAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0003824 catalytic activity molecular function None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (NzwP) rather than this protein.
PDB ID
NzwP
Method AlphaFoldv2
Resolution 90.76
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50