Protein

Protein accession
A0A8S5LYP7 [UniProt]
Representative
3A548
Source
UniProt (cluster: phalp2_19529)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSNSKLVNYTKLSPNHSGTRTHSIDRITPHCVVGQCSVETLGNIFMNKKSEASCNYGIGTDGRVLLCVDEDNRSWCSSSNSNDQRAVTIECASELKHPYAFNDKVYNKLVELCVDICKRNGKKKLLWINDKNKALNYSTKSDEMLLTVHRWFDNKSCPGDWLYNRLGNIAKSVTDKLSGKVNKVEKPTLTYRVFADHKWYSEVKGLSNIAGRKKQAISAVAVKVSKGNIKYRVHLLNGDWLPWVDGYDIKDDINGYAGIKGKVIDGLQVEFEGVGDYKATYRVRKQGAGFWDWQHNTEKDSSQDGYAGLLGTKIDGLQITLT
Physico‐chemical
properties
protein length:322 AA
molecular weight:36172,6 Da
isoelectric point:9,08
hydropathy:-0,56
Representative Protein Details
Accession
3A548
Protein name
3A548
Sequence length
503 AA
Molecular weight
55538,01620 Da
Isoelectric point
8,83251
Sequence
MAYTNSPLVAYTKLSPNHSGQRTHAIDRITPHCVVGQCSVETLGNIFYPTSRQASCQYGIGVDGRVGMYVEEKNRSWCSSSNANDQRAITIECASDTTHPYAFKDVVYEKLIELCVDICKRNGKSKLLWLGDKTKTLNYSPKSDEMVLTVHRWFANKSCPGDWLYNRLGDLAKQVTDKLGGTTVTPATPETSTDGNDKVIWDYLKGKGLNDYAVAGIMGNLYAESGFKPTNLQITYEKKLGYTDATYTAAVDNGTYTNFVKDSAGYGLVQWTYWSRKQALLAHAQSVGKSIGDFTMQLDFMWEEMQGYKSMMTTLAGATSILEASNAVLTQYERPADQGETVQKKRAGYGQTYYDKYAQKATATDGLYRVQVGAFSKLDNANRQLAAVQAKGFDALIKKVGNLYKVQIGAYSVKSNAEAQLTRVKAAGFTDAYITTESGGTVVATTPQEEKPAYITYTVKKGDTLWDIAKKYLGSGSRWKEIYNLSGLTSQTIYAGQKLKIPD
Other Proteins in cluster: phalp2_19529
Total (incl. this protein): 66 Avg length: 454,7 Avg pI: 8,69
Protein ID Length (AA) pI
3A548 503 8,83251
13GRp 502 8,96228
13rHn 507 8,98620
15WRw 547 9,36108
19fQ4 455 9,12777
1kAW7 455 8,00673
1kvbZ 446 8,85456
21nG8 454 9,15227
21pu2 456 8,67237
21x8J 430 9,02482
237n6 511 8,95738
23EAh 400 7,25546
23EOu 430 8,50946
23HNT 449 9,12990
23J26 430 8,84553
23Ka0 430 8,87216
23t0j 472 8,69738
23uPs 443 9,06975
24LQ5 502 8,66289
2TtaO 437 6,63842
2XK5Z 502 8,99329
2bAVr 455 9,35612
2bpiq 499 8,64349
2pUK3 428 8,93656
3TKFt 456 8,84540
3TKah 440 9,25961
3TMme 440 9,35128
3WHeQ 642 8,84804
3WKW7 416 7,96895
3WL04 343 8,44415
3WMRZ 456 9,20185
3WN5U 445 9,05660
3WNYH 415 7,09711
3d3X2 516 9,05956
3gWUd 455 9,16014
3h5XP 422 8,27737
3iEbM 470 9,23292
3iwi4 455 8,97292
414WJ 483 9,02366
419uX 423 8,54923
41l5y 456 9,32601
41lKW 481 8,97601
41lo1 416 8,41320
41nKX 456 9,11888
4L5od 408 6,16182
4L5y6 424 7,96367
4L8St 457 9,15014
4klFC 436 6,98650
5jzgs 422 6,93057
5o4j 447 9,17122
6rYC5 471 8,69513
6rqGE 437 8,83618
7DSEo 415 8,27299
7JzbJ 454 9,13106
7MZY6 505 8,98994
88mVr 501 8,25236
8fBmu 442 9,35825
8k4eC 453 9,36972
8pteB 503 8,76540
8uaQN 501 8,58424
a5pP 454 7,97611
goEV 504 8,96963
ouT0 452 8,81497
ovtq 458 8,96093
A0A8S5MEY6 312 9,24569
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_20479
3TRRD
 962 67,1% 377 1.890E-187
2 phalp2_17251
3TIE6
 53 52,0% 405 3.491E-160
3 phalp2_18866
1JeL2
 29 47,4% 527 8.876E-157
4 phalp2_27209
3o7E3
 7 39,6% 452 1.760E-94
5 phalp2_31507
3VJ9A
 7 40,4% 363 3.076E-84
6 phalp2_10739
3iXeP
 1 23,8% 645 7.523E-77
7 phalp2_8982
3PMSU
 4 42,4% 311 4.909E-74
8 phalp2_4221
3vCra
 4 38,1% 333 9.256E-72
9 phalp2_23416
6fsej
 1 28,7% 389 9.806E-50

Domains

Domains [InterPro]
Ami2
Phage_lys2
SPOR
LysM
Representative sequence (used for alignment): 3A548 (503 AA)
Member sequence: A0A8S5LYP7 (322 AA)
1 503 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01476, PF01510, PF05036, PF18013

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctA995
[NCBI] 		2826180 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK014769 [NCBI]
CDS location
range 39546 -> 40514
strand +
CDS
ATGAGTAATTCAAAACTTGTAAACTACACAAAATTAAGCCCGAACCATTCAGGAACAAGAACACACAGTATTGACCGCATTACTCCTCACTGTGTTGTCGGTCAGTGTTCGGTTGAAACTCTTGGTAACATTTTTATGAATAAAAAAAGTGAAGCAAGCTGTAACTACGGCATCGGTACAGACGGCAGGGTTCTCCTCTGTGTTGATGAGGACAACCGTTCGTGGTGTTCTTCTTCAAACTCAAACGACCAGAGAGCCGTTACAATTGAATGTGCAAGCGAGCTTAAACACCCTTACGCATTTAACGATAAAGTTTATAATAAGCTGGTTGAGCTTTGCGTTGACATCTGCAAGCGTAACGGCAAGAAAAAGCTTCTGTGGATTAACGATAAAAACAAGGCTTTAAATTATAGCACTAAGTCAGACGAAATGCTCCTGACCGTTCACAGGTGGTTTGATAACAAGTCCTGCCCAGGCGACTGGCTTTATAACCGCCTTGGTAACATTGCAAAAAGCGTTACAGACAAGCTCAGTGGCAAGGTTAATAAAGTTGAAAAGCCAACGTTGACTTATCGTGTATTTGCTGATCACAAATGGTACAGCGAAGTAAAAGGTCTTAGCAACATAGCAGGAAGAAAAAAGCAAGCTATTTCGGCAGTTGCAGTAAAGGTCAGCAAGGGCAACATCAAGTACAGAGTGCATCTGCTCAACGGTGACTGGTTGCCTTGGGTTGACGGATACGACATCAAGGACGATATCAACGGCTATGCAGGCATTAAAGGCAAGGTTATTGACGGCTTGCAGGTTGAATTTGAGGGAGTCGGCGATTATAAGGCTACATACAGAGTGCGTAAGCAGGGTGCAGGCTTTTGGGATTGGCAGCACAACACAGAAAAAGACAGCTCACAGGACGGTTACGCAGGACTTCTCGGCACTAAAATTGACGGCTTGCAGATTACTTTGACTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (3A548) rather than this protein.
PDB ID
3A548
Method AlphaFoldv2
Resolution 89.38
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50