Protein

Protein accession
A0A7D7FP48 [UniProt]
Representative
543gw
Source
UniProt (cluster: phalp2_6086)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 95% (predicted by ML model)
Protein sequence
MRIVIDTDEIKTFPKKVWNRMTPEHVILFAMFGILSIPALIDKYDSSNQEGSCCVDVVLEKPEPIKPVKPPIPEKTKFVRIFNTDKKVNLSEKEFDCLSRNIYWESLREPLIGQIAVSQITYNRVKSKKWGNTFCKVVFQPKQFSWTNDKHIVNRTPKNTKQWERAKHSALLFTKGVRVPNLKDSEFYYAEYIDPPKWSKNMKKEAHIGRHIFFSNKRKEKMK
Physico‐chemical
properties
protein length:223 AA
molecular weight:26308,4 Da
isoelectric point:9,71
hydropathy:-0,63
Representative Protein Details
Accession
543gw
Protein name
543gw
Sequence length
196 AA
Molecular weight
22440,44430 Da
Isoelectric point
9,24175
Sequence
MTLNQKYLAAGVAIFVAQVLYHQWITDERIAVIEDAVVAIAEDVQDIKQAVIVEQDRAVIKYSKQEFDCLARNIYYEAGVEDKLGKIAVAQVTLNRSRTGYWGRDICKVVYAPAQFSWTRVKRRAWLQNTGANWVESRQVAAEVLDQGIRIPTLKTSLFYHADYIKDPYWADHTKRTTKVGRHIFYTQAKGGTLKL
Other Proteins in cluster: phalp2_6086
Total (incl. this protein): 128 Avg length: 197,1 Avg pI: 9,21
Protein ID Length (AA) pI
543gw 196 9,24175
12H6Z 175 9,43716
12LHX 183 8,47703
14tju 178 7,00793
165YR 225 9,30023
17Tfd 189 9,37681
18vFG 175 9,42645
18x0u 196 9,62128
1AE9K 194 9,64848
1AJC4 174 9,43174
1LFmI 217 9,44238
1NW9v 172 9,71089
1OdB7 178 9,08019
1YThn 179 9,21977
1hEzF 181 9,28172
1hqyy 233 9,56442
1iNmb 175 9,39364
1ioLS 198 9,06698
1q2Iz 211 7,02117
1r3H0 229 9,35277
1roqt 194 9,83370
1rpln 193 9,67827
20cpv 175 9,38249
25DJE 170 9,11198
2DN3q 192 9,26548
2Dw1z 197 8,47264
2EEyl 178 9,42136
2FhCz 207 9,91267
2LvSo 178 9,57615
2Nxdt 215 9,40138
2bDu9 181 9,45037
2bE8c 191 8,71930
2bHa2 215 9,46797
2bKDy 179 9,27263
2bx7J 187 9,74267
2j4fm 226 9,53160
2jgk0 222 9,64881
2xdtg 199 8,87325
30Xdb 175 9,36979
30ipz 175 9,56126
31It3 226 9,57015
329b6 222 7,77332
36WC5 184 8,81420
37IVa 207 8,75772
3RNx4 208 6,44335
3b3DU 169 10,09499
3b71d 176 9,42143
462yi 183 9,01250
46HXQ 188 9,59742
46z3e 175 9,34110
47oNY 175 9,25568
48060 179 8,55400
48YSF 172 9,03545
4961f 176 9,38294
4FFNB 200 9,62927
4V8Pz 189 9,65338
4aSvp 207 7,68238
4d2bm 213 9,30100
4d5xm 178 8,87241
4dfud 174 9,35973
4dh7R 192 9,19282
4dmXB 192 9,39390
4fy4Z 194 9,59710
4nCpP 189 9,58705
4xRGg 180 7,97076
501ll 190 5,35602
501vK 197 9,60877
52DTY 204 9,71431
59nEP 227 9,42568
59xP5 217 9,08142
5a33p 192 9,50066
5bRV9 177 9,51839
5gEle 202 9,51516
5gr8I 189 9,73616
5gtXi 189 9,53515
5kORw 178 9,24311
5kYaS 164 8,44634
5o8LZ 189 9,67285
5ouZN 172 9,73706
5thHh 224 9,56912
6AEX8 220 9,71527
6KWhb 184 9,45321
6LMkD 224 9,44218
6M1A7 186 9,65925
6QvWH 207 9,72630
6yAh6 189 9,56835
7F1Kj 189 9,71598
7K3yC 213 9,39274
7L6pI 178 9,26135
7M3UP 179 9,22499
7TuI2 232 9,76833
7WoG1 279 9,57905
80LQA 209 9,44992
82QDq 230 9,89566
82kSL 222 9,76214
84ttJ 222 9,87277
87u1d 171 8,35518
891Qw 212 5,95953
8Djr9 199 8,68081
8E9sO 231 9,87303
8EOxZ 196 8,37207
8EaP6 221 9,39467
8Ghlt 232 9,76833
8GwU 192 9,58808
8Ldv 211 9,77761
8hGcj 234 10,06018
8kF4M 231 9,90204
8mDFt 193 9,03919
8pxcV 209 9,29913
8tjED 224 8,82303
8wHtf 199 8,87325
8x57I 221 9,33775
8xp5L 231 9,90204
8y1WA 234 9,99913
8yEY6 142 5,24541
8zBth 222 9,76801
Iy3u 178 9,33149
RLZp 209 9,84215
RU9G 171 8,44015
SEdp 186 9,33323
TGQK 173 9,02598
THXH 176 9,49408
XCmn 164 9,61103
bCFl 169 9,41994
mxq0 182 8,97363
A0A7D7F283 226 9,53431
A0A7D7F4C8 232 9,54514
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_13600
5lmUm
 9 37,6% 199 5.333E-60
2 phalp2_15462
87Yrl
 67 43,0% 158 6.624E-59
3 phalp2_15984
7KpQ6
 448 42,3% 137 2.516E-40
4 phalp2_21419
85EIE
 1722 43,8% 130 4.248E-39
5 phalp2_3334
38dW3
 10 42,2% 142 3.804E-35
6 phalp2_9879
2oYX8
 442 34,8% 155 3.804E-35
7 phalp2_2416
54zSE
 377 39,3% 127 5.205E-35
8 phalp2_4108
1zenp
 45 32,2% 186 3.415E-34
9 phalp2_19867
5DmT3
 416 41,1% 124 1.197E-33
10 phalp2_38710
V6TC
 7 31,8% 135 1.197E-33

Domains

Domains [InterPro]
Unannotated
Hydro_2
Representative sequence (used for alignment): 543gw (196 AA)
Member sequence: A0A7D7FP48 (223 AA)
1 196 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF07486

Taxonomy

  Name Taxonomy ID Lineage
Phage Caudoviricetes sp
[NCBI] 		2832643 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MT773556 [NCBI]
CDS location
range 731 -> 1402
strand -
CDS
ATGAGAATTGTAATTGATACTGATGAAATCAAGACCTTTCCTAAGAAGGTCTGGAATCGTATGACTCCTGAGCATGTTATACTGTTTGCGATGTTTGGGATTCTATCTATTCCTGCTTTAATAGATAAATACGATTCTTCGAATCAAGAAGGCTCTTGTTGTGTTGATGTTGTATTAGAAAAACCGGAACCGATTAAACCGGTAAAACCTCCTATTCCCGAAAAAACCAAGTTTGTTAGGATTTTTAATACTGATAAAAAAGTAAATCTTTCTGAAAAAGAATTTGATTGCCTTTCCAGAAACATTTATTGGGAATCGTTAAGAGAACCCTTAATTGGACAAATTGCTGTTTCACAAATAACATACAATAGAGTTAAATCCAAAAAGTGGGGTAATACTTTCTGTAAAGTGGTATTTCAACCGAAACAATTTTCATGGACAAATGATAAACATATTGTAAATAGAACTCCCAAAAATACGAAACAATGGGAAAGAGCTAAGCATTCGGCTTTATTATTTACAAAAGGAGTTAGAGTTCCTAATTTAAAAGATTCAGAGTTTTATTATGCGGAATATATTGATCCGCCAAAGTGGTCCAAAAATATGAAAAAAGAAGCTCATATTGGAAGACATATTTTCTTTAGTAATAAGCGCAAGGAGAAAATGAAATGA

Gene Ontology

Description Category Evidence (source)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. N/A UniProt

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (543gw) rather than this protein.
PDB ID
543gw
Method AlphaFoldv2
Resolution 91.02
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50