Protein

Protein accession
A0A6N3IKS1 [UniProt]
Representative
8ftop
Source
UniProt (cluster: phalp2_4322)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MLKDKARVVENTLSYIPKPGTMVIFNNSYGCGHGHVAWVLSANQNQIIVIENNWLGGGWTWGDAQGGGGWEKATIRAHGYDFPMWFIEPNFKDEVQTTWNWRGKFTADRTIKVRRTPGLSGSIVGAESFIYSGQYVNFDQVIKADGYWWIRFKYPTNPSAGNFYMAVCKITDKWERMLKEKYWGRINWK
Physico‐chemical
properties
protein length:189 AA
molecular weight:21832,6 Da
isoelectric point:9,39
hydropathy:-0,47
Representative Protein Details
Accession
8ftop
Protein name
8ftop
Sequence length
235 AA
Molecular weight
26753,41200 Da
Isoelectric point
9,76330
Sequence
MYTDTAYGERIGNHKDYGMYWVHKQKYDIVVELHLDAASASASGGHVIISNQWPADKIDKDINNCLKATVGTIRNIDPRNDLLNANVAGRLGTNYRLVEMGFITNKKDMNYLKKNYDKFSKELAGAINGKPIGGTSAGSKQITWNWKGRFTANTTIKVRRKPGLSGAVVDKGSWIYKNQWVDFASVTKKDGYWWIKFKYPTNPKAGYFYMAICKITDKNEKLKKEKNLFGKIKYK
Other Proteins in cluster: phalp2_4322
Total (incl. this protein): 34 Avg length: 275,4 Avg pI: 9,42
Protein ID Length (AA) pI
8ftop 235 9,76330
7ryli 332 9,88966
A0A1W6JPE2 460 9,48390
A0A1P8CMK4 252 9,08380
A0A0U1ZWR4 252 9,25742
A0A1D6Z273 456 9,67975
A0A7U0GE02 460 9,54301
M4I0E5 252 9,21455
A0A455VCU1 252 9,27102
A0A7T8C2A2 252 9,35412
I6TG35 460 9,50240
A0AAE7SUB7 462 9,81056
A0A1X9SIB5 189 9,39312
A0A6N3IVY5 189 9,39312
A0A976XQS9 248 9,02198
A0A976XRF9 189 9,39312
A0A977KJS3 248 9,07168
A0A9E7SR51 248 9,28488
A0A9E7U5H8 248 9,07143
A0A9E9P506 252 9,16510
A0AA94WZ37 252 9,27102
A0AAD1NZC1 247 9,85336
A0AAD1PU32 189 9,39312
A0AAE9VV74 252 9,17793
A0AAF0AHR6 252 9,24452
A0AAF0D8T5 248 9,01934
A0AAX4B4E1 259 9,72765
A0AAX4B5C2 259 9,76549
A0AAX4B5E2 259 9,68181
A0AAX4B6K4 259 9,76549
A0AAX4LWK0 248 9,19108
A0AAX4NUY5 265 10,05637
I7CCV5 248 9,19108
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_29175
7A7gP
 25 45,7% 225 6.227E-64
2 phalp2_13095
8tcB0
 3 54,9% 153 1.987E-53
3 phalp2_3137
88Bj8
 3 28,8% 187 4.928E-05

Domains

Domains [InterPro]
Ami3
Unannotated
Representative sequence (used for alignment): 8ftop (235 AA)
Member sequence: A0A6N3IKS1 (189 AA)
1 235 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage KSAP7
[NCBI] 		2599601 Herelleviridae > Silviavirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LC492751 [NCBI]
CDS location
range 128466 -> 129035
strand -
CDS
ATGTTAAAAGATAAAGCTAGAGTAGTAGAAAATACACTATCCTACATTCCTAAACCAGGCACTATGGTTATCTTTAATAATTCATACGGGTGTGGTCATGGTCATGTCGCATGGGTACTAAGTGCAAACCAAAACCAAATTATAGTAATTGAAAATAATTGGTTAGGTGGAGGTTGGACTTGGGGAGATGCTCAAGGAGGCGGTGGCTGGGAAAAAGCTACTATTAGAGCCCATGGTTACGACTTTCCTATGTGGTTTATAGAACCTAATTTCAAAGACGAGGTACAAACAACTTGGAATTGGAGAGGTAAATTTACTGCTGATAGAACGATTAAGGTAAGACGAACACCAGGTTTAAGTGGTTCAATCGTAGGAGCTGAATCATTTATTTATAGTGGTCAATATGTAAACTTTGACCAAGTTATTAAAGCAGATGGCTATTGGTGGATTAGATTTAAATATCCTACAAACCCGTCAGCTGGGAACTTCTATATGGCAGTATGTAAAATCACTGACAAGTGGGAACGTATGCTAAAAGAGAAGTATTGGGGTCGTATTAATTGGAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0003824 catalytic activity molecular function None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi000a31b5f3_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (8ftop) rather than this protein.
PDB ID
8ftop
Method AlphaFoldv2
Resolution 95.19
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50