Protein

Protein accession
A0A6J5SET3 [UniProt]
Representative
4Rwju
Source
UniProt (cluster: phalp2_15958)
Protein name
N-acetylmuramoyl-L-alanine amidase domain
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MNLVQSKVRFQATGMHEPHTITLHHTAGGLKGSEAYLKQKGLGYHFMIAKDGTVYAYNPVDEIVSHSARANRGFIGVSYEAGGPLGPVNEAQLASSIELVTVLANDCDTIRKVSDHASVDNMIAHRGWKSDPQWPGEKAEVNDWTIKHKFLDTIARASGLVAFKYEPNAVRSRIPLDVDLFDNDDNGDENDGGCLC
Physico‐chemical
properties
protein length:196 AA
molecular weight:21435,7 Da
isoelectric point:5,66
hydropathy:-0,39
Representative Protein Details
Accession
4Rwju
Protein name
4Rwju
Sequence length
165 AA
Molecular weight
17874,33980 Da
Isoelectric point
8,42513
Sequence
MKLVNKLGKLASHGRQEPYLIILHHTAGPNLGGAEATLKQRGLGYHYMIDNNGDCHEYAKPTSMMYHAGGYNRGTVGISFVGGGDAPPASEVQIKALIELINTRVKPLGPDLIQITCHKHASHSGKIDPRWPGEPSNGISLGIDRIYMERIAKAVCLEFLGHGEV
Other Proteins in cluster: phalp2_15958
Total (incl. this protein): 12 Avg length: 172,2 Avg pI: 7,98
Protein ID Length (AA) pI
4Rwju 165 8,42513
4AMzB 195 10,40470
4AYFo 147 10,03800
4BqTg 167 9,54140
4eQSd 165 6,48711
4en89 161 8,96776
4fXAH 163 8,70692
5kZRK 163 8,69403
6Y5Yp 183 5,57758
8pOyU 165 7,86464
TUoP 196 5,37068
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_39380
4H4oF
 116 36,6% 109 4.657E-16
2 phalp2_26524
85UVS
 3 29,0% 117 1.619E-15
3 phalp2_11760
45aqa
 242 31,3% 118 1.048E-14
4 phalp2_672
1oD9I
 6 26,5% 147 3.198E-13
5 phalp2_2008
2dyt3
 7 27,8% 122 5.948E-13
6 phalp2_14372
4kZ6h
 10 27,8% 140 2.803E-12
7 phalp2_10505
89jAF
 60 25,5% 149 3.820E-12
8 phalp2_19793
4W5p2
 179 27,6% 112 2.446E-11
9 phalp2_35354
7vHgi
 29 28,1% 128 3.332E-11
10 phalp2_25442
2RRxP
 189 28,5% 105 4.539E-11

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 4Rwju (165 AA)
Member sequence: A0A6J5SET3 (196 AA)
1 165 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage uncultured Caudovirales phage
[NCBI] 		2100421 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
LR797382 [NCBI]
CDS location
range 28371 -> 28961
strand +
CDS
ATGAACTTAGTTCAAAGTAAGGTTCGATTCCAGGCTACTGGTATGCATGAACCCCACACAATAACATTACATCACACTGCAGGCGGCTTAAAGGGCTCTGAGGCCTATTTAAAGCAGAAAGGCTTAGGTTATCATTTTATGATTGCTAAAGATGGCACAGTGTACGCGTACAACCCTGTAGATGAGATTGTCTCTCATTCCGCTAGGGCCAATCGTGGCTTTATTGGAGTGTCGTATGAGGCTGGAGGTCCATTAGGCCCTGTCAATGAGGCACAACTTGCTTCGTCTATTGAACTAGTCACTGTGCTTGCTAACGACTGCGATACTATCCGTAAAGTCTCAGATCATGCTTCAGTGGATAATATGATAGCTCATAGAGGCTGGAAATCTGACCCACAATGGCCAGGAGAAAAGGCTGAAGTGAATGATTGGACGATTAAACATAAGTTTCTAGATACTATTGCTAGAGCTAGTGGTTTAGTTGCATTTAAATATGAACCTAATGCTGTCAGATCTAGAATACCTTTAGATGTTGATTTATTCGATAATGACGATAACGGCGATGAAAATGATGGAGGTTGCTTATGTTAA
CDS Source ID
[NCBI]
CDS Source
LR798383 [NCBI]
CDS location
range 15312 -> 15902
strand -
CDS
ATGAACTTAGTTCAAAGTAAGGTTCGATTCCAGGCTACTGGTATGCATGAACCCCACACAATAACATTACATCACACTGCAGGCGGCTTAAAGGGCTCTGAGGCCTATTTAAAGCAGAAAGGCTTAGGTTATCATTTTATGATTGCTAAAGATGGCACAGTGTACGCGTACAACCCTGTAGATGAGATTGTCTCTCATTCCGCTAGGGCCAATCGTGGCTTTATTGGAGTGTCGTATGAGGCTGGAGGTCCATTAGGCCCTGTCAATGAGGCACAACTTGCTTCGTCTATTGAACTAGTCACTGTGCTTGCTAACGACTGCGATACTATCCGTAAAGTCTCAGATCATGCTTCAGTGGATAATATGATAGCTCATAGAGGCTGGAAATCTGACCCACAATGGCCAGGAGAAAAGGCTGAAGTGAATGATTGGACGATTAAACATAAGTTTCTAGATACTATTGCTAGAGCTAGTGGTTTAGTTGCATTTAAATATGAACCTAATGCTGTCAGATCTAGAATACCTTTAGATGTTGATTTATTCGATAATGACGATAACGGCGATGAAAATGATGGAGGTTGCTTATGTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (4Rwju) rather than this protein.
PDB ID
4Rwju
Method AlphaFoldv2
Resolution 96.32
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50