Protein

Protein accession
A0A125V787 [UniProt]
Representative
75Uho
Source
UniProt (cluster: phalp2_10022)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQGNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGTEGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANESGYDYIARLITEGILNKDINM
Physico‐chemical
properties
protein length:180 AA
molecular weight:20401,1 Da
isoelectric point:5,97
hydropathy:-0,36
Representative Protein Details
Accession
75Uho
Protein name
75Uho
Sequence length
282 AA
Molecular weight
31770,22820 Da
Isoelectric point
8,23263
Sequence
MKICITVGHSILKDGNCTSASGVVNEYQYCKALAPVLTNLLKKEGHTVDMIICPEIQFTKATEEKDYKLPKINGKKYDLCLELHLNSFNKTAKGAEVLYVSNSGKIYAERVMKKLATLFTNRGVKLRNDLYILNGTDCPTILLETFFCDNAKDYRVAKDKNYEGVAKLIAEGILNKTISDSDSNSKPSESLGSNSLHKNCVLYEGTVDWHIAEIFSWYLEDCALVKVDNFKSYTANNLYVIGEIAEKKLIEKNLPDRYTTFAGIDRFETLDKALAFINLKRK
Other Proteins in cluster: phalp2_10022
Total (incl. this protein): 6 Avg length: 265,0 Avg pI: 8,24
Protein ID Length (AA) pI
75Uho 282 8,23263
74pI4 282 8,93121
74sNp 282 8,74315
7Pxlc 282 8,74315
8IF9b 282 8,84611
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_19905
69yEp
 107 44,2% 201 7.678E-52
2 phalp2_28871
5hUC8
 21 34,0% 197 3.359E-31
3 phalp2_22023
5iret
 1 36,8% 179 1.162E-28
4 phalp2_23331
5tGuH
 43 36,3% 190 7.331E-28
5 phalp2_27800
7z5TK
 19 28,9% 287 4.545E-25
6 phalp2_35186
67E6C
 93 33,5% 182 1.542E-24
7 phalp2_36719
6eCmw
 993 33,5% 182 2.396E-23
8 phalp2_7005
82zuE
 2 31,4% 191 2.015E-22
9 phalp2_2703
7cyBj
 192 32,6% 196 2.015E-22
10 phalp2_19930
6wdij
 24 31,2% 176 2.375E-18

Domains

Domains [InterPro]
Ami3
Unannotated
Representative sequence (used for alignment): 75Uho (282 AA)
Member sequence: A0A125V787 (180 AA)
1 282 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520

Taxonomy

  Name Taxonomy ID Lineage
Phage Peptoclostridium phage phiCDIF1296T
[NCBI] 		1677909 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
CP011968 [NCBI]
CDS location
range 2383016 -> 2383558
strand -
CDS
ATGAAAATATGCATAACAGTAGGTCATAGTATACTAAAAGGTGGTAAAAGTACAGGTGTAAATGGTATAGTTGATGAATACAATTATAATAAAAAATTAGCTCCTATGTTAGCTGAGATGCTTATAAGTCAAGGTAATACAGTAGATGTAATAATTTGTCCAGAAAGGCACTTTATGTCAGAAAGAGAAGAATTTTTCTATAGAGTTCCAAAAGTAAACAGTGGTAAATACGATTTATTAGTAGAGCTACATTTAAATAAAGCTGATGGAACACAATGTGGTACAGAAGTGCTTTACTATGGCACTGAAGGATTAGAATATGCTAAAAGAGTATCTAATAGACTTGGAGAATTATTTGAAAATAGAGGAGCTAAAAAAAGAGAAAATTTATATATATTGAGAAATACAAATCCAGTTGCAATACAGATTGAAAGTTTTTTCTGTGATAATGTGAATGATTGTAACAAAGCAAATGAATCAGGTTATGATTATATAGCACGATTGATTACAGAAGGTATATTGAATAAAGATATAAATATGTGA

Gene Ontology

Description Category Evidence (source)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)

Enzymatic activity

No enzymatic activity data available.

Tertiary structure

PDB ID
A0A125V787
Method AlphaFoldDB
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi00006dca45_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (75Uho) rather than this protein.
PDB ID
75Uho
Method AlphaFoldv2
Resolution 92.47
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50