Protein

Protein accession
D6QWP4 [UniProt]
Representative
7kqpf
Source
UniProt (cluster: phalp2_8205)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MRLLKNGTKIGNATVIVDIVDGANREIRPRTKMNPRFLTDHDTGNSGNGADAKSHNRYIHNLGDKLPRDTSHISWHVTVDENFIIQHIPFDECAYHCGDGWATTSGNRTSIGIEKCMHRGADRNKIEANAIALYAYLMKELKIPITSVRPHQHWSGKYCPQLILNRYGSFTPFRNKIEAAFKGGAVTAAKPSTVKRDYLLDGDTGAAVKTLQSELKQAGFLLSVDGIFGKGTETAVKAFQRANGLAVDGVFGTGSQAKLNAILANLNKKPVVKPAAPTKPKEESTVEKTKQPSKWAEVTIKEAVKIGVSDGSRLHDTVTREESIVIAMRAAGLAPRLK
Physico‐chemical
properties
protein length:338 AA
molecular weight:36892,7 Da
isoelectric point:9,61
hydropathy:-0,41
Representative Protein Details
Accession
7kqpf
Protein name
7kqpf
Sequence length
329 AA
Molecular weight
36905,16920 Da
Isoelectric point
9,43903
Sequence
MRFLPHGMKIGHATVIVDIVPKGNPKIRPGIPMKARGITDHDTGNPGRGADAKAHNKLLHNWGKLPVKDTTHISWHGALDEDFIIQHLPFDEPAYHCGDGWGLNSGNRNTIGFEKCMHQGADRQKIEENAIALYAYLMKEMNLPIEWVRPHQYWSGKYCPQLILNKYGTFKPFRDKIEAEFKRTKVKVAQVSKDYWMNGDKGKPIEIIQDMINFLGYNPNLTVDGIFGNGTEAGVRWFQKKFNLVIDGIAGKATLSKLTAEVNAVKKVEVDKKAAEEAKKAAVAKPKAPVYRVSVEGKQVNAYSQLTGMVQELEKAIKSGKKNIEYKQV
Other Proteins in cluster: phalp2_8205
Total (incl. this protein): 6 Avg length: 330,5 Avg pI: 9,43
Protein ID Length (AA) pI
7kqpf 329 9,43903
1muMF 306 9,35986
35n23 342 8,72994
7m67O 356 9,15382
XGrj 312 10,29317
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_29544
3KzTO
 225 36,8% 247 5.925E-62
2 phalp2_29138
7cEL4
 14 31,9% 269 1.239E-47
3 phalp2_17898
EGF4
 130 26,8% 291 3.562E-35
4 phalp2_3982
7wtdu
 333 26,6% 319 2.592E-33
5 phalp2_6450
8Jtqs
 137 32,6% 242 2.202E-32
6 phalp2_17464
4UG80
 8 28,3% 261 1.261E-27
7 phalp2_38227
7x2xX
 21 28,9% 273 1.177E-25
8 phalp2_7264
3WBGP
 11 29,0% 244 5.308E-22
9 phalp2_36672
45lpM
 229 27,8% 298 2.365E-21
10 phalp2_33823
1lgPb
 23 25,3% 241 6.266E-20

Domains

Domains [InterPro]
Ami2
PG_1
Unannotated
Representative sequence (used for alignment): 7kqpf (329 AA)
Member sequence: D6QWP4 (338 AA)
1 329 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01471, PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage uncultured phage
[NCBI] 		278008 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
HM011618 [NCBI]
CDS location
range 1 -> 1017
strand +
CDS
ATGCGCTTACTAAAAAATGGCACAAAAATAGGAAACGCCACAGTAATTGTTGATATTGTGGACGGCGCAAACAGAGAGATTCGACCGCGCACAAAAATGAATCCGCGTTTCTTGACGGACCACGACACGGGTAACTCGGGTAATGGCGCAGACGCGAAAAGTCATAATCGCTACATCCACAACTTGGGCGATAAGCTACCACGCGACACAAGCCATATTTCCTGGCATGTAACTGTGGATGAAAACTTCATCATTCAACATATCCCATTTGACGAGTGTGCTTATCATTGCGGCGACGGTTGGGCGACGACTAGCGGGAATCGCACAAGTATCGGCATAGAGAAGTGCATGCACAGAGGCGCGGATCGAAATAAGATTGAGGCCAACGCGATTGCGCTTTATGCGTATCTAATGAAGGAACTCAAAATACCGATTACATCTGTACGCCCTCATCAGCATTGGAGCGGCAAATACTGTCCACAGTTGATATTGAATCGGTACGGTTCGTTTACGCCATTCCGCAATAAGATTGAAGCGGCGTTTAAAGGTGGCGCAGTAACGGCAGCGAAGCCTTCCACAGTGAAGAGAGACTACCTATTAGATGGCGATACAGGCGCGGCGGTTAAAACGCTACAAAGCGAATTGAAACAAGCAGGTTTCTTACTATCTGTGGATGGCATATTCGGAAAAGGTACAGAGACAGCGGTTAAGGCGTTTCAACGTGCGAATGGATTAGCAGTTGATGGCGTGTTCGGAACGGGCTCACAGGCTAAACTCAACGCAATACTGGCGAATCTGAATAAGAAGCCGGTAGTTAAGCCGGCAGCGCCAACAAAACCAAAGGAGGAATCCACAGTGGAGAAAACAAAACAACCCTCAAAATGGGCAGAAGTGACGATTAAAGAAGCGGTGAAGATTGGTGTATCGGACGGATCTCGCTTGCATGATACAGTGACTCGTGAAGAATCAATTGTAATCGCCATGCGCGCTGCAGGACTTGCGCCGAGACTTAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0001d1d783_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7kqpf) rather than this protein.
PDB ID
7kqpf
Method AlphaFoldv2
Resolution 91.36
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50