Protein

Protein accession
D6QWN2 [UniProt]
Representative
7cMO8
Source
UniProt (cluster: phalp2_13827)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MLTVQQIIRQGGKRLISHPSDYQVAFLMEIFMAYELRDNRRSTYLAKGRANTAIDVIVIHHWGVDGQNWENLTTYTANNRNMSTHYVAMAGKVERQVDEEDTAYHGGNPPINQRSIGIECRPEATDGDYDTVAELVADIWNRHGKLPLVGHKQVPSVRPGQQYVATSCPGRYDVERIRKEAEGWYTKKYAKKDSDARPKTHTVVSGESYWSIAAKYLGDGMRYTEILDLNKVKDPSALLVGQVLELPEKYAKH
Physico‐chemical
properties
protein length:253 AA
molecular weight:28709,0 Da
isoelectric point:7,77
hydropathy:-0,63
Representative Protein Details
Accession
7cMO8
Protein name
7cMO8
Sequence length
238 AA
Molecular weight
26167,19570 Da
Isoelectric point
8,69480
Sequence
MYTYETQHTARSFSYGREGNRPRYIVIHHWGADGQTHSGVVDFFENRAETSAHYVASAGRVTCLVACSNTAYHAGTWQANLESIGIECHPEMSAGDLETVAELIADIRRAYGYLPLRGHRDIVPTACPGRYYTRLAWLDQRARQIQSGAILPRPNPATKPQQGKGTRGMFMLALKEGNGLRFAIVAPNFWLEFTGQSAANNLNRQLCGGAALICTRAFWDYCKAEALAGRAPQAGDAA
Other Proteins in cluster: phalp2_13827
Total (incl. this protein): 12 Avg length: 244,8 Avg pI: 7,44
Protein ID Length (AA) pI
7cMO8 238 8,69480
1RhFd 267 6,50513
3A4HY 269 5,31413
3jTvW 240 9,07439
69UXi 196 4,92308
75Zn4 264 6,34053
7ubku 243 5,83318
7wgmY 252 9,58795
7wgnY 257 9,02933
7wgnt 191 9,66989
uh1o 267 6,48802
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_16559
7s5Uq
 12 58,0% 155 4.203E-59
2 phalp2_28305
13CeQ
 161 46,3% 207 3.763E-52
3 phalp2_7211
3lM9H
 3 48,3% 178 1.171E-47
4 phalp2_13682
65xN2
 64 46,2% 158 5.023E-46
5 phalp2_31988
59Np4
 130 50,3% 153 5.357E-41
6 phalp2_7901
8LrgT
 13 47,9% 167 1.368E-40
7 phalp2_25234
40uRC
 5 41,4% 169 2.557E-40
8 phalp2_6225
6oM64
 106 31,3% 172 3.495E-40
9 phalp2_30585
5Zqsx
 178 34,0% 176 1.220E-39
10 phalp2_36900
7w7ps
 19 40,5% 202 1.667E-39

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 7cMO8 (238 AA)
Member sequence: D6QWN2 (253 AA)
1 238 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage uncultured phage
[NCBI] 		278008 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
HM011606 [NCBI]
CDS location
range 1 -> 762
strand +
CDS
ATGTTGACGGTCCAGCAGATCATCCGGCAGGGTGGCAAGAGATTGATTAGCCACCCCAGCGACTATCAGGTCGCTTTTTTAATGGAGATTTTTATGGCATACGAATTGAGAGACAATAGACGCTCTACCTACCTGGCTAAAGGCAGAGCCAATACAGCGATTGACGTCATTGTTATCCATCACTGGGGTGTTGACGGTCAAAATTGGGAGAACTTAACCACCTACACGGCCAATAACCGCAATATGAGTACCCATTACGTTGCCATGGCTGGCAAGGTCGAGCGACAGGTGGACGAGGAGGATACGGCTTACCATGGTGGCAATCCACCGATCAATCAACGCTCTATCGGCATTGAGTGCCGTCCAGAAGCCACAGACGGCGATTACGACACAGTGGCTGAGCTGGTGGCTGATATCTGGAACAGGCACGGCAAGCTACCCTTGGTCGGCCATAAGCAAGTGCCATCCGTCAGGCCGGGTCAGCAATACGTGGCGACAAGCTGCCCGGGCAGATACGATGTGGAGCGGATCCGTAAAGAAGCGGAGGGTTGGTACACGAAGAAGTACGCCAAGAAGGACAGCGACGCACGACCTAAGACGCACACGGTCGTGAGTGGCGAATCCTACTGGAGTATTGCAGCCAAGTACCTAGGCGATGGCATGAGGTACACAGAAATATTAGACCTGAACAAGGTCAAAGATCCGAGTGCCTTATTAGTCGGACAGGTCTTGGAACTGCCTGAAAAGTACGCTAAGCACTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0001d1d76b_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7cMO8) rather than this protein.
PDB ID
7cMO8
Method AlphaFoldv2
Resolution 84.02
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50