Protein

Protein accession
D6QWN1 [UniProt]
Representative
81fVt
Source
UniProt (cluster: phalp2_37291)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MYQITRDYIKFGNSRCGQQIKKVLFIVSHDTGNPGSTAYGNRNYFNNQQPSASAHTFIDDKYILEIIPIYEKAWHVQYQKPKDNQMFGDDANDAAIGVELCWGNGINFNEAYKRFVWYHAHLCKTFKLNPRKHIVSHKTLDPERKVDPIDCFKRHGITWEQFINDVENVFVGKKEENDMLEKAILIGGFPDFAVAEILAARLKAPIYTRDAYPGGKVTKELFVVGGSTAGLQADKVINLSGKDRFAVAAAVKKFIG
Physico‐chemical
properties
protein length:256 AA
molecular weight:28982,7 Da
isoelectric point:8,86
hydropathy:-0,38
Representative Protein Details
Accession
81fVt
Protein name
81fVt
Sequence length
276 AA
Molecular weight
30173,40630 Da
Isoelectric point
9,18573
Sequence
MLKITHACGHALTTSGKQSPDGYKEWSYTNEIVKLIMQELENYEGVSQKRIDDPTGKTDYPLVVRSNMVNSFNPNLHIDYHMNAFGSGWTAPGGTETFVYKKTLKEAVNIATKVQNNLVNSLGFANRGVKEGDLHMIRETNPTAILIEFAFMSNREEAVLMRSAEYQRKAAFAVVDAIVSQYGLKRKSIPVQQKGKIEMLEKAIVIGGFPDFAVAEILAARLKAPVYTRAALPGGKIAKEVYVVGGSVEGIQGDKVISLTGADRFAVAAAVKKFIG
Other Proteins in cluster: phalp2_37291
Total (incl. this protein): 2 Avg length: 266,0 Avg pI: 9,02
Protein ID Length (AA) pI
81fVt 276 9,18573
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_23918
1NTlO
 42 39,7% 191 1.424E-35
2 phalp2_9307
7sfyx
 22 39,5% 187 4.293E-34
3 phalp2_35303
72wI1
 17 36,8% 190 2.016E-33
4 phalp2_32087
63Erl
 43 35,6% 230 2.130E-27
5 phalp2_13925
foQI
 206 33,3% 210 2.261E-20
6 phalp2_12153
6dhq0
 1 37,0% 186 1.881E-19
7 phalp2_31486
3PO9t
 18 29,4% 204 1.733E-17
8 phalp2_24413
4m1OU
 1246 30,0% 233 3.062E-14
9 phalp2_35652
1qFqy
 5 25,7% 221 4.122E-14
10 phalp2_7949
zu48
 832 32,9% 179 4.423E-13

Domains

Domains [InterPro]
Ami3
Unannotated
Representative sequence (used for alignment): 81fVt (276 AA)
Member sequence: D6QWN1 (256 AA)
1 276 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520

Taxonomy

  Name Taxonomy ID Lineage
Phage uncultured phage
[NCBI] 		278008 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
HM011605 [NCBI]
CDS location
range 1 -> 771
strand +
CDS
ATGTACCAAATCACAAGGGATTACATTAAATTCGGTAATTCTCGATGCGGGCAACAAATTAAGAAAGTGTTGTTTATCGTAAGCCATGACACAGGGAATCCTGGTAGTACAGCATACGGGAATCGAAATTACTTTAATAATCAACAACCTAGTGCGTCAGCCCACACGTTCATTGATGATAAATACATTTTGGAGATCATCCCTATCTATGAGAAAGCATGGCACGTACAGTATCAAAAACCAAAAGATAATCAAATGTTTGGTGATGATGCGAATGATGCAGCTATCGGGGTTGAACTTTGTTGGGGGAATGGTATCAACTTCAATGAAGCATACAAGCGTTTTGTGTGGTATCATGCTCATCTTTGTAAGACATTTAAACTCAATCCTAGAAAGCACATCGTATCTCATAAGACATTGGATCCAGAACGTAAAGTAGACCCGATAGACTGCTTTAAACGTCATGGTATTACATGGGAACAATTCATAAATGATGTAGAAAATGTGTTTGTAGGGAAAAAGGAGGAAAATGATATGTTAGAAAAAGCGATTCTAATAGGTGGATTCCCTGATTTTGCAGTAGCGGAGATATTAGCAGCACGATTAAAAGCACCTATCTACACTCGTGATGCTTATCCGGGTGGAAAGGTTACAAAAGAATTATTTGTAGTCGGTGGTTCTACCGCAGGCCTACAAGCAGACAAAGTAATCAATCTATCCGGAAAAGACCGATTTGCTGTTGCTGCAGCAGTTAAAAAGTTTATCGGATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0001d1d76a_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (81fVt) rather than this protein.
PDB ID
81fVt
Method AlphaFoldv2
Resolution 93.76
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50