Protein

Protein accession
A0AAX4QXQ3 [UniProt]
Representative
2aQ1n
Source
UniProt (cluster: phalp2_20292)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MSYINNGVIKNGSKIGTATVDSSGLLKKGWMIPYVSFSPSSVTIHETDMPNVSSEQIYKSLKNGNSDTSRKQASFQICVSATKIMQCVNLYRTCWHAGNRTGSSTSIGIEICQYDDKALQEKAYKNAAELVKIILAEIKTVKKVVQHNYWSGKNCPSKLRARWKGYTWDWFTNLVYSKKKYTKLKNGDYNKKATVLTPTLNVRKSRPDKNGKLGKYDFKLKEGDIIEVGYVLNGWASIWVEGDMGYINTSSKYIKLL
Physico‐chemical
properties
protein length:257 AA
molecular weight:29003,0 Da
isoelectric point:9,65
hydropathy:-0,49
Representative Protein Details
Accession
2aQ1n
Protein name
2aQ1n
Sequence length
262 AA
Molecular weight
30161,53190 Da
Isoelectric point
9,15988
Sequence
MVKVVNNLSYNNNYYNILNTRQYITIHETSNTSVGANADAHANYINNGGSQETWHYTVDDTKAVKHYKHTTSCWHAGTYAGNTQSIGIEMCVNQDGNYQKTLQNTIELVKEIMRIEHIPASNVVQHNYWSGKNCPMLLRSGQYGMTWTGFKSQLKQKSKVGIWTVNQHDTYYRKRHGVFKVGNEPIITRIGSPYANKNVKKGQHGKVGEEIEFHTACIQDGYYWLEFTNENRQEEFIPVCKTKGVPLHQTYGQLWGQYIKKK
Other Proteins in cluster: phalp2_20292
Total (incl. this protein): 8 Avg length: 242,6 Avg pI: 9,28
Protein ID Length (AA) pI
2aQ1n 262 9,15988
1oy3G 254 9,27631
2ZJF 200 9,17264
4SCrO 199 8,96235
5PMOi 252 9,45437
5RaY9 252 9,45437
6wvW1 265 9,13641
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_18764
13cSu
 4 54,9% 273 1.593E-121
2 phalp2_7826
aMK
 29 48,4% 190 7.199E-61
3 phalp2_38352
A4V0
 20 36,2% 182 8.508E-43
4 phalp2_3173
8mQDL
 1 33,3% 258 6.689E-41
5 phalp2_33474
7qPh3
 3 31,8% 261 3.835E-39
6 phalp2_24299
3nIle
 36 36,5% 167 1.191E-30
7 phalp2_38485
1mrCF
 6 36,2% 174 5.587E-30
8 phalp2_39055
7ordh
 68 29,6% 192 1.966E-27
9 phalp2_3370
3jRfu
 70 33,5% 185 7.901E-24
10 phalp2_5052
1q1Q3
 64 31,6% 180 1.073E-23

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 2aQ1n (262 AA)
Member sequence: A0AAX4QXQ3 (257 AA)
1 262 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Terrisporobacter phage TPDSL_ph2
[NCBI] 		3141807 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
PP197247 [NCBI]
CDS location
range 20077 -> 20850
strand +
CDS
ATGAGTTATATTAATAATGGAGTAATAAAGAATGGATCTAAAATAGGAACTGCTACTGTTGATAGTTCTGGATTATTAAAAAAAGGATGGATGATACCTTATGTATCATTTTCACCATCATCTGTTACAATACATGAGACAGATATGCCTAATGTATCTTCAGAACAAATATACAAATCATTAAAGAATGGAAACAGTGATACAAGTAGAAAACAAGCATCTTTTCAGATTTGCGTATCTGCAACAAAAATAATGCAGTGTGTTAATTTATATAGAACTTGTTGGCATGCAGGAAATAGAACAGGAAGTTCAACTAGTATAGGTATAGAAATATGCCAATATGATGATAAAGCATTACAAGAAAAAGCTTATAAGAATGCAGCTGAATTAGTAAAAATTATATTAGCAGAAATTAAAACAGTTAAAAAAGTAGTACAACACAACTATTGGAGCGGAAAGAACTGTCCAAGTAAATTAAGAGCTAGATGGAAAGGTTATACTTGGGATTGGTTTACTAATTTAGTATATAGTAAAAAGAAATATACTAAGCTTAAAAATGGAGACTACAACAAGAAAGCAACTGTATTAACTCCTACACTTAATGTAAGAAAATCAAGACCAGATAAGAATGGAAAACTTGGTAAATATGATTTCAAATTAAAAGAAGGAGATATTATAGAAGTAGGATATGTTCTTAATGGGTGGGCTTCTATTTGGGTAGAAGGTGACATGGGATATATCAATACTAGTAGTAAGTATATAAAATTATTATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (2aQ1n) rather than this protein.
PDB ID
2aQ1n
Method AlphaFoldv2
Resolution 94.01
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50