Protein

Protein accession
A0AAX4QWU6 [UniProt]
Representative
68WMu
Source
UniProt (cluster: phalp2_11198)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 98% (predicted by ML model)
Protein sequence
MSKITNGVIKNGIKIGYATVISTDLLKAGHAIPYVSYKPTSITIHETDCPDVSAKTIYKSLSNANVDTTRAKASFQITVDADNIRQCVNLLRTCWHAGDATGNKTSIGIEICQYSKDKEKQKKAYQNTAELVKILKSELGIKKVYRHYDWTKKNCPSYMLNKKYSGLTWDWFTNLLTTKQYKQLLNGDYNKKYKVISNTLNVREARPDKNGNLAKVKFVLKEGAIVEVGYVYNGWASIWVEGDMGYINTSNKYITLL
Physico‐chemical
properties
protein length:257 AA
molecular weight:29049,0 Da
isoelectric point:9,47
hydropathy:-0,44
Representative Protein Details
Accession
68WMu
Protein name
68WMu
Sequence length
255 AA
Molecular weight
28768,22830 Da
Isoelectric point
9,32156
Sequence
MKNNRLDETKIGNATVIVDIVPKGHCIPNVKITPTTITIHNTGNNNASAKNNHNYMKNINKSGERKASWHFTVDDIEIYQAQSTNFKCYHAGTTSGNNTSIGIEICQFTDANKQLKAYENTIELVKILLKYHNFTIDKVVQHNHWSGKDCPRLLRTNKFGYNWDWFIKQCKEITSQGSSTIAFKKNGDYTGKKAKVTATALNVRYDRGTQYNVIGKLKKGDVVNLSYCLNGWISIEGYNGNKGLGYVSTDYLELI
Other Proteins in cluster: phalp2_11198
Total (incl. this protein): 78 Avg length: 260,3 Avg pI: 9,29
Protein ID Length (AA) pI
68WMu 255 9,32156
1gIOo 256 9,75860
2EgL 257 9,27096
2d8NF 245 9,32066
2fZno 247 9,40602
3PPaQ 247 9,49344
3PZ3O 326 9,24820
3jKsR 257 9,59491
3nEHu 247 9,38268
3nM4i 245 9,63378
3nRfm 260 9,81810
3s5AQ 268 9,50446
4ErgL 241 8,95738
4VBbf 255 9,32866
5JTFR 257 9,29043
5KjAx 264 9,26696
5LU6X 253 9,39235
5LrZb 254 9,39261
5Otie 254 9,24807
5PNYI 257 9,29043
5PYqJ 257 9,28050
5Qyuc 257 9,29043
5R62v 291 10,02118
5V55n 240 9,56442
5VwkD 258 9,16594
5Zb2g 254 9,29049
5ZblA 259 9,21603
636yh 256 9,31957
63v0v 259 9,27760
66GdL 254 9,31118
6814J 257 9,27083
68Zpy 259 9,24324
6Z3RC 257 9,32924
6Z4B7 254 9,18953
6aX39 257 9,38384
6c7Wv 257 9,32924
6eU4Z 267 9,41285
6fgxV 257 9,31957
6fp1g 254 9,32169
6gYWa 260 9,18476
6hdTR 257 9,28063
6hpGz 258 9,20546
6iC9L 254 9,34864
6iQwv 259 9,27760
6k2Ji 256 9,31957
6k2N3 254 9,50433
6kQ8z 257 9,18515
6kmcz 257 9,22647
6l8Dx 254 9,44386
6l8ls 254 9,40331
6mEsA 254 9,35947
6nuGe 256 9,16594
6oa6q 258 9,10688
6p3VC 256 9,28050
6pueo 256 9,33929
6sHGR 254 9,42555
6vhbh 253 9,34335
6w6n9 252 9,32156
727w8 257 9,58627
7aQNJ 257 9,57164
7aQOO 257 9,58034
7eySM 292 7,14474
7f8Rg 257 9,60458
7fE1Z 288 7,70995
7i9V9 292 7,14133
7su7t 314 8,70305
85lPu 254 9,34851
8bBI5 257 9,28050
8heZU 242 9,11617
8kiY9 255 9,46043
8kiZD 261 8,99303
8lrJT 267 9,42284
8sXOm 254 9,37249
8tZ8P 329 9,47938
8tZed 256 9,65732
aHlr 291 10,04722
q3g2 257 9,61283
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_20199
83ZcC
 8 32,0% 262 2.531E-51
2 phalp2_9318
7vPuw
 15 38,9% 167 1.481E-49
3 phalp2_39055
7ordh
 68 38,1% 186 4.627E-48
4 phalp2_22264
7pxLN
 64 31,7% 236 3.686E-46
5 phalp2_29544
3KzTO
 225 44,2% 174 1.021E-43
6 phalp2_33479
7swgL
 97 46,6% 163 1.696E-42
7 phalp2_3987
7ylun
 65 37,6% 223 2.318E-42
8 phalp2_15067
7vQ2g
 6 34,3% 227 3.167E-42
9 phalp2_22227
73VY4
 57 34,8% 172 2.497E-40
10 phalp2_11225
6wIAx
 104 33,0% 239 3.410E-40

Domains

Domains [InterPro]
Ami2
SH3_3
Representative sequence (used for alignment): 68WMu (255 AA)
Member sequence: A0AAX4QWU6 (257 AA)
1 255 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08239

Taxonomy

  Name Taxonomy ID Lineage
Phage Terrisporobacter phage TVCOM_ph1
[NCBI] 		3141809 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
PP197245 [NCBI]
CDS location
range 21758 -> 22531
strand +
CDS
ATGAGTAAAATAACTAATGGAGTAATAAAAAATGGAATAAAAATAGGATATGCTACAGTAATAAGCACAGATTTATTAAAGGCTGGTCATGCAATACCATATGTGAGTTATAAACCTACATCAATTACAATACACGAAACAGATTGTCCTGATGTAAGTGCAAAGACTATATATAAGTCATTATCAAATGCAAATGTTGATACAACAAGAGCAAAGGCAAGTTTTCAAATAACTGTAGATGCAGATAATATAAGACAATGTGTTAATTTATTAAGAACATGTTGGCATGCTGGAGATGCAACAGGTAACAAGACCTCTATAGGAATAGAAATCTGCCAATATTCGAAAGATAAAGAAAAACAAAAGAAAGCTTACCAAAATACTGCAGAGTTAGTAAAAATATTAAAATCAGAACTTGGTATAAAAAAAGTATATAGACATTATGACTGGACAAAAAAGAACTGTCCATCTTATATGTTAAATAAGAAATACTCAGGGCTTACTTGGGATTGGTTTACTAATCTATTAACTACTAAACAATATAAACAATTGCTAAACGGCGATTATAACAAAAAATATAAAGTTATAAGTAATACTTTAAATGTAAGAGAAGCTAGACCAGATAAAAATGGTAATCTAGCTAAAGTTAAATTTGTACTTAAAGAAGGAGCTATAGTAGAGGTAGGATATGTTTATAATGGCTGGGCTAGCATATGGGTAGAAGGTGATATGGGTTACATAAATACTAGCAATAAGTATATAACATTATTATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (68WMu) rather than this protein.
PDB ID
68WMu
Method AlphaFoldv2
Resolution 94.07
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50