Protein

Protein accession
A0AAX4QWP0 [UniProt]
Representative
7skBJ
Source
UniProt (cluster: phalp2_27786)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSISKPKVIEKWQKRNKYGRPGTYLKPTKIAIHYTGEADVPGKNTVSYFNNVVANGYKVNGKYVYASAHYVIDLDGTIYQLIPTTERCYATNSANSYAIGIEVATTGKDNHYTDETYKAMAHLCAWLCQYKGLDCKKDIITHTDVVGKSYKECPKYMVRNPKKMEQFKLDAYNLKAEKIKVPDIKNCTNGKGEVTKVPGTSATTPPKDTWKNGDYNCKVKATANLNARKGRGTNNPVIYTIPKGTEFTVGYVYNNWGSTWDFKGQVLYICCDYIEKA
Physico‐chemical
properties
protein length:277 AA
molecular weight:31182,3 Da
isoelectric point:9,27
hydropathy:-0,62
Representative Protein Details
Accession
7skBJ
Protein name
7skBJ
Sequence length
242 AA
Molecular weight
27608,76980 Da
Isoelectric point
9,11597
Sequence
MEIKLLTINQYSRPGKKMGPIKNIVIHWVGNAGSSAMANRNYFESLKEKKFFASSHYIVGLDGEIIQCIPEDEIAYHANNANSYSLGIEVCHPDWIGKFSDVTYKALIGLLADLCKRYNLEPTTAIIRHYDVTKKLCPKYYVENNGAWLQLKQDVKVAIGTEDTELKKAAELLYKRNIISVLSAWADMSKFKLEYVPGLLKNMGGIDRLVKDKIISDRLLWEYKQYNANHVRALIIKYSKLG
Other Proteins in cluster: phalp2_27786
Total (incl. this protein): 83 Avg length: 243,5 Avg pI: 8,24
Protein ID Length (AA) pI
7skBJ 242 9,11597
1GKPA 235 5,91099
1GoHR 235 6,65973
1HrGT 210 8,93527
1NfEH 212 9,08613
2bUM3 235 6,60426
2mvG8 257 9,19895
2nSWk 228 7,00315
3Asg2 245 8,93624
3B6m1 245 8,78828
3JrqA 242 8,36537
3wW9p 242 8,83431
4TuYD 268 9,15008
4ZlHX 250 8,48335
4ucno 250 7,05147
4xssU 227 9,18960
4ykXe 222 6,52542
5KPpa 245 8,97659
5LA8D 243 8,10401
5LtwA 243 8,11156
5Mi5p 245 9,02946
5PhE3 248 8,87635
5Q44U 242 7,06869
5RtLr 245 8,88170
5TnTW 245 9,01689
5Vgso 245 8,89034
5W5rG 244 8,87319
5WHke 242 8,36440
5Ycfj 245 8,93656
5YcrA 245 8,83947
5YoU2 245 9,13931
5hSUK 277 6,25942
5i1uq 249 6,32779
5i2ZD 233 5,24791
5i3QU 247 5,46430
5ipSZ 223 7,68096
60Mne 243 8,75579
60mA0 245 9,03952
61ae0 267 7,56649
62gpX 244 8,39902
64V7U 245 9,14060
66Itp 245 8,74380
66XoQ 245 8,75888
677ln 245 7,61457
6bTRO 245 8,75882
6cnbV 245 8,89943
6gaM5 245 8,75888
6hFpW 242 7,07381
6ifLa 244 8,75141
6l6fO 245 8,98594
6mK2S 245 8,39825
6n8aW 243 8,75579
6nHik 242 8,56728
6oRKh 195 7,69693
6pbrz 245 9,03474
6przz 245 8,65509
6roTJ 245 8,82413
6wmcG 243 8,61615
758qd 315 8,24030
7C1fv 254 8,84675
7C1gv 244 9,11604
7JCcT 270 8,84663
7XORi 266 7,59883
7XqA8 267 8,04864
7rTS7 245 8,85726
7rf7c 240 5,54433
7rgyo 214 8,23256
7skAB 230 6,71225
7skCI 230 7,08335
7x2BV 252 6,09970
7xzIo 217 9,12584
7xzJA 214 9,13602
859oR 226 8,91728
8jHfY 263 7,10097
8kQ9p 244 8,96338
8kmX2 266 8,04477
8lEU0 245 8,93540
8ofh5 243 8,75173
8s5W3 245 8,60248
8tqoK 280 8,06308
NiNY 194 8,98207
R9b4 227 9,32272
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_3939
7bfSL
 153 54,7% 201 2.545E-74
2 phalp2_25288
86peP
 187 56,4% 163 2.130E-63
3 phalp2_7626
5U1PP
 11 51,8% 162 4.929E-62
4 phalp2_29669
8sfID
 1005 30,3% 211 1.541E-51
5 phalp2_23587
3s1m
 2 37,8% 267 5.400E-51
6 phalp2_37528
3iFdV
 13 36,4% 173 1.240E-49
7 phalp2_26646
2dQ36
 4 36,1% 202 1.492E-45
8 phalp2_29527
23SbO
 38 34,9% 166 2.951E-40
9 phalp2_5052
1q1Q3
 64 41,4% 181 6.699E-39
10 phalp2_23523
75EkF
 74 43,0% 172 3.190E-38

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 7skBJ (242 AA)
Member sequence: A0AAX4QWP0 (277 AA)
1 242 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Terrisporobacter phage TPELB_ph1
[NCBI] 		3141808 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
PP197244 [NCBI]
CDS location
range 19269 -> 20102
strand +
CDS
ATGAGTATATCAAAACCAAAAGTAATAGAAAAATGGCAAAAGAGAAATAAATACGGAAGACCAGGAACTTATTTAAAGCCTACAAAAATAGCAATACATTATACTGGTGAAGCAGATGTTCCTGGTAAAAATACAGTAAGTTATTTCAATAATGTTGTTGCAAATGGATATAAAGTAAATGGAAAATATGTATATGCTTCAGCTCATTATGTAATTGATTTAGATGGGACTATATATCAATTAATTCCAACAACAGAAAGATGTTATGCAACAAATAGTGCAAATAGTTATGCAATAGGTATAGAGGTAGCAACAACAGGAAAGGATAATCACTACACAGACGAAACATATAAGGCTATGGCCCATTTATGTGCTTGGTTATGTCAATATAAAGGATTAGATTGTAAGAAAGACATAATAACTCACACAGATGTTGTAGGTAAGAGCTACAAGGAGTGTCCAAAGTATATGGTCCGTAATCCAAAGAAGATGGAACAATTCAAGCTAGATGCTTATAATCTTAAAGCAGAAAAAATAAAAGTACCAGATATAAAAAACTGTACAAATGGAAAAGGTGAAGTAACAAAAGTTCCTGGTACTTCAGCTACAACTCCACCAAAAGACACTTGGAAAAATGGGGATTATAACTGTAAAGTAAAAGCTACAGCTAATTTAAATGCTAGGAAAGGAAGAGGAACTAATAATCCTGTTATTTATACCATTCCAAAAGGAACAGAATTTACAGTAGGTTATGTATATAATAACTGGGGCAGTACTTGGGATTTTAAAGGACAAGTATTATATATTTGTTGTGATTATATAGAAAAAGCATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7skBJ) rather than this protein.
PDB ID
7skBJ
Method AlphaFoldv2
Resolution 95.85
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50