Protein

Protein accession
A0AAX4QB24 [UniProt]
Representative
8diNj
Source
UniProt (cluster: phalp2_15488)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTRFYKGIAGRRGANPSGVVIHNDAGSVYANAAFYRGWLPSHDAENGFAHYYVASDGTFQAEDEMNMAWHTANSTGNAYYIGIEACQSMGPENIFRQNEENSIKLAAQILKRYGLQPNRNTVKLHKQFSPTSCPHRSVALHGDGYVMQDYFIARIKSYMGSNPTPAPTPAPSTGTRKYNIDLVNINSKAFQIKGWFTPAKATKGQDIWVYIMKKGGPEIARFKAKKIQRPDVQKAVSNPNGADVGFQVDAPTPEGVLGKDFDLLLRYNNDNKEEIRTKEHWKAPALINEGYLDKVAGDAKGVTFAGWHLNTRQKDGFRHYLFVTDAKTGKEYVRFDITGGSYLASPDIAKKYGPEIAQRGNCRFHNWCALPANHPARGKTVNIISRYAPAKEIDTKTSTDKLWGTYRL
Physico‐chemical
properties
protein length:408 AA
molecular weight:45525,8 Da
isoelectric point:9,46
hydropathy:-0,61
Representative Protein Details
Accession
8diNj
Protein name
8diNj
Sequence length
182 AA
Molecular weight
19578,78070 Da
Isoelectric point
9,49447
Sequence
MITKGIAGRRNGKVKGVVIHNDAGSNAANVSFYKGYVTNNNAANGFAHYYIASDGMVQMEDEANMAWHTANSDGNANYIGIEACQSLGDKKQFLANEAKAIKLAAEILKRHGLQPNKATVRLHNSFSATACPHRSQEIHGPGAKTQDYFINEIKKHMGGSAPAGKPHQSPAGSIDKFEKKGR
Other Proteins in cluster: phalp2_15488
Total (incl. this protein): 63 Avg length: 254,2 Avg pI: 6,76
Protein ID Length (AA) pI
8diNj 182 9,49447
1mZgT 245 6,38333
1nQE8 199 5,28127
1orzn 146 6,95075
22db1 250 8,10479
2HwS 245 5,90702
3IeDB 245 5,98841
3KN46 246 6,04263
3evwG 164 6,64194
3kFLs 246 5,83665
3kHYd 247 6,77961
3mbrc 247 6,35229
3opxA 251 5,90889
3sD6O 246 5,90827
3wTRY 245 5,77509
3wb0N 153 5,61140
3wxac 246 5,91713
3yWCF 240 5,79351
3yWPP 244 6,39793
3yX5f 246 4,91097
5Kr42 251 5,75815
5LzRd 242 6,54566
5NQJ4 246 5,80380
5QDlt 324 7,09620
5UMl0 248 6,24839
5WibC 181 5,19550
5Wlfi 235 7,63697
5XQqC 208 6,69605
5XTN7 173 6,63791
60PCd 179 5,75798
63yMP 251 5,90906
67Tg3 245 5,29418
6Yy75 251 6,69804
6c1w1 233 5,51619
6fyMR 233 6,19019
6lJP4 247 6,24276
6rpq3 233 5,63800
6w240 251 5,91418
6wmY8 251 6,24504
79LnA 240 5,98335
7BfFA 245 5,61822
7WYgQ 251 6,06935
7WZuN 247 6,24680
7h7cg 247 7,12002
7mr7F 242 6,74027
7t7tZ 246 5,65118
7tQRj 247 6,49285
7uCTA 246 5,90554
7v6mn 145 6,35258
80P60 246 6,35081
8MoP0 233 6,65280
8r8jq 174 5,94868
Oxd7 334 6,81365
A0A0S2MYH4 304 9,67189
A0A411B6T3 304 9,60091
A0A249XZR2 408 9,48744
A0A9E7J2Z5 304 9,60091
A0AAE9FFY1 304 9,54585
A0AAE9P442 406 9,42078
A0AAX4Q8Y2 406 9,45527
A0AAX4Q9D3 406 9,45527
A0AAX4Q9G1 406 9,45527
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_32258
7xeAb
 253 54,9% 171 9.770E-76
2 phalp2_36839
6Z1Os
 11 39,2% 163 8.634E-36
3 phalp2_36896
7vCZc
 30 35,5% 169 7.017E-34
4 phalp2_15359
1El58
 3 32,6% 187 8.650E-33
5 phalp2_20237
8tSRS
 1 40,8% 147 3.036E-32
6 phalp2_24730
6BIOG
 3 26,6% 184 1.884E-22
7 phalp2_8333
8JaN1
 18 40,9% 122 1.675E-21
8 phalp2_25038
13C0m
 57 32,8% 125 1.744E-14
9 phalp2_13879
7z7dc
 255 27,0% 155 4.486E-12
10 phalp2_6225
6oM64
 106 26,5% 177 8.295E-12

Domains

Domains [InterPro]
Ami2
Disordered region
Representative sequence (used for alignment): 8diNj (182 AA)
Member sequence: A0AAX4QB24 (408 AA)
1 182 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Enterococcus phage VRE9_4
[NCBI] 		3141818 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
PP680570 [NCBI]
CDS location
range 94884 -> 96110
strand +
CDS
ATGACTCGTTTTTATAAAGGAATCGCTGGTCGTCGAGGAGCTAATCCATCAGGAGTAGTTATTCACAATGATGCAGGCTCTGTTTATGCTAATGCTGCATTTTATCGTGGATGGTTACCTTCTCATGATGCAGAAAATGGTTTTGCTCATTATTATGTAGCTAGCGATGGAACATTCCAAGCAGAAGATGAAATGAATATGGCATGGCATACTGCAAATTCAACTGGTAATGCGTATTATATCGGTATTGAAGCATGTCAGTCAATGGGACCAGAAAATATTTTTCGACAAAATGAAGAAAATTCTATTAAATTAGCAGCCCAAATCTTAAAGAGATATGGTTTACAACCTAATAGAAATACTGTAAAATTGCACAAGCAATTTTCACCAACTTCTTGTCCTCACCGTTCTGTTGCTTTACATGGTGATGGATACGTAATGCAAGATTATTTTATTGCTCGTATTAAATCTTATATGGGTTCTAATCCAACTCCAGCCCCAACCCCAGCACCAAGTACTGGAACTAGAAAATATAATATTGACCTAGTTAATATCAATTCAAAAGCTTTCCAAATCAAAGGTTGGTTTACCCCAGCTAAAGCTACGAAAGGTCAAGATATTTGGGTTTATATTATGAAAAAAGGTGGACCAGAAATTGCCCGCTTTAAAGCGAAAAAAATTCAACGTCCAGACGTTCAAAAAGCAGTTTCAAATCCTAACGGAGCAGATGTTGGTTTCCAAGTAGATGCTCCTACACCAGAAGGCGTTCTAGGGAAAGATTTTGATTTGTTGCTTCGTTACAATAACGATAACAAAGAAGAAATTCGCACTAAAGAGCATTGGAAAGCTCCTGCATTAATTAACGAAGGATATCTAGACAAAGTTGCTGGAGATGCTAAAGGTGTTACATTTGCTGGATGGCATCTAAATACACGTCAAAAAGATGGATTTAGACATTATCTATTTGTAACTGATGCAAAAACTGGAAAAGAATATGTACGATTCGATATTACAGGAGGGTCTTACTTAGCTTCTCCAGATATCGCTAAGAAATATGGTCCAGAAATCGCTCAACGTGGTAACTGCCGCTTCCATAATTGGTGCGCATTACCAGCAAATCATCCAGCTAGAGGTAAAACTGTTAATATTATTAGCCGTTATGCTCCTGCAAAAGAAATTGATACAAAAACTTCTACAGACAAACTGTGGGGAACTTATAGACTATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8diNj) rather than this protein.
PDB ID
8diNj
Method AlphaFoldv2
Resolution 88.29
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50