Protein

Protein accession
A0AAF0FL87 [UniProt]
Representative
7A7gP
Source
UniProt (cluster: phalp2_29175)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MERLEHNTKGQCFDLANFYYNYVVGSGSLAGQGAKDIPFQNNFKGIAKVHKNTPAFKAQAGDIVVFSGKYGGGYGHVAIVTNGNYDGNYMKFQSLDQNWYGGGLARTEVAQKIVHNYDFPMWFIRFDFKPETAKSTTKKKATKKKATVKKETAKKVKKRKIMLVAGHGYNDPGAVANGENERDFIRKNITKRVKSYLEKAGHEVALYGGSKQDQNMFQDTAYGQNIGNRKDYGLYWVKNQGYEVVVEFHLDAASPSASGGHTIIASGVKADSIDTGIQNAIKKHVGTIRGITGRANLLNCNVSKEVNINYRLVELGFITNKSDMNNIKKNLDAYCKDIAEAISGGAIKGGVPKSTATKSTAKPKQAKTSSSTSGWNYNKTVGSYWKSEKATFTCTTPKGIVTRYNSPTLRSQKAGLLRKGQSVVYDEIAIKDGYVWIHWIAYGGSHIWMPVRPCVGTPPNHKVKGAWGTFK
Physico‐chemical
properties
protein length:471 AA
molecular weight:51883,4 Da
isoelectric point:9,76
hydropathy:-0,56
Representative Protein Details
Accession
7A7gP
Protein name
7A7gP
Sequence length
356 AA
Molecular weight
39228,40210 Da
Isoelectric point
9,86542
Sequence
MWFIRFDFKPETAKSTTKKKATKKKTTAKKATVKKETAKKVKKRKIMLVAGHGYNDPGAVANGENERDFIRKNITKRVKSYLEKAGHEVALYGGSKQDQNMFQDTAYGQNIGNRKDYGLYWVKNQGYEVVVEFHLDAASPSASGGHTIIASGVKADSIDTGIQNAIKKHVGTIRGITGRANLLNCNVSKEVNINYRLVELGFITNKSDMNSIKKNLDAYCKDIAEAISGGAIKGGVSKSSATKSTAKPKQAKTSSSTSGWNYNKTVGSYWKPENLTFTCTTPKGIVTRYNSPTLRSQIAGLLRKGQSVVYDEIAIKDGYVWIHWIAYGGSHIWMPVRPCVGTPPNQIVKDAWGTFK
Other Proteins in cluster: phalp2_29175
Total (incl. this protein): 25 Avg length: 349,6 Avg pI: 9,65
Protein ID Length (AA) pI
7A7gP 356 9,86542
4160a 472 9,86961
7quCn 472 9,78645
7sQaM 260 9,58750
7vBUM 210 9,50395
7vBUT 194 9,31770
7vBVS 242 9,42304
7vypc 263 9,25445
7ycDS 472 9,78632
A0A2R3ZXN5 356 9,86542
H9A0X4 483 9,54714
A0A1X9I9Y6 293 9,82448
A0A0H4TFZ2 171 9,63520
A0A0E3T6Y3 171 9,63520
A0A0N9SGX6 500 9,38668
A0A4Y6EB79 290 9,60155
A0A7U0G8N8 297 9,85188
A0AA49X2T7 487 9,63198
A0AA50AFH3 483 9,55275
A0AAF0D5P0 487 9,64700
A0A898KB35 461 9,72501
A0AA48XBU3 297 9,78226
A0AAU6MXP0 256 9,56197
A0AB39C8B8 297 9,90475
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_22245
7e47k
 317 58,7% 332 2.293E-143
2 phalp2_4322
8ftop
 34 44,1% 265 1.402E-68
3 phalp2_16525
72rfr
 1 27,4% 372 4.025E-39
4 phalp2_2541
6fZPr
 29 29,6% 219 7.232E-24
5 phalp2_5157
238De
 36 29,5% 230 1.489E-13
6 phalp2_37478
366XE
 2 22,8% 389 3.507E-12

Domains

Domains [InterPro]
Disordered region
Ami3
SH3_5
Representative sequence (used for alignment): 7A7gP (356 AA)
Member sequence: A0AAF0FL87 (471 AA)
1 356 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520, PF08460

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage vB_SauM_JDF86
[NCBI] 		3038179 Herelleviridae > Baoshanvirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OQ129426 [NCBI]
CDS location
range 103402 -> 104817
strand -
CDS
ATGGAAAGGTTAGAACATAACACAAAAGGGCAATGTTTTGATTTAGCAAACTTCTATTATAACTATGTAGTAGGTAGTGGAAGTTTAGCAGGACAAGGAGCAAAAGACATTCCTTTCCAAAATAATTTCAAAGGTATAGCTAAAGTACACAAAAATACTCCTGCATTTAAAGCACAAGCAGGGGATATTGTGGTATTTTCTGGAAAATACGGTGGCGGATATGGTCACGTAGCTATTGTAACAAATGGAAATTATGATGGAAACTATATGAAGTTCCAATCACTAGACCAAAATTGGTATGGTGGAGGATTAGCACGAACAGAAGTTGCACAAAAAATTGTACACAACTATGATTTTCCTATGTGGTTTATTCGTTTTGACTTTAAACCAGAAACAGCTAAGAGCACAACTAAGAAAAAAGCTACTAAGAAGAAAGCGACAGTTAAAAAAGAAACAGCTAAAAAAGTTAAAAAACGTAAAATTATGTTGGTAGCAGGTCATGGGTATAACGACCCTGGAGCTGTAGCTAACGGGGAAAATGAAAGAGACTTCATTAGAAAAAATATTACTAAACGTGTTAAATCATATTTAGAAAAAGCAGGACATGAAGTTGCTTTATATGGTGGTTCTAAACAAGACCAAAATATGTTCCAAGATACAGCATATGGACAAAATATTGGAAATAGAAAAGACTATGGATTATATTGGGTTAAAAACCAAGGTTATGAAGTCGTAGTAGAATTCCATTTAGATGCAGCAAGCCCTTCAGCAAGCGGTGGACATACAATTATTGCTTCTGGAGTTAAAGCAGACAGTATTGATACTGGAATACAAAATGCTATTAAGAAACATGTTGGAACTATTCGAGGAATTACTGGACGAGCAAACTTATTAAACTGTAACGTTTCTAAAGAAGTTAATATTAACTATCGTTTAGTAGAATTAGGATTTATTACTAATAAATCTGATATGAATAATATTAAGAAAAATTTAGATGCTTATTGTAAAGATATTGCAGAAGCTATTAGTGGTGGAGCTATTAAAGGCGGAGTTCCAAAATCAACAGCTACTAAATCAACAGCTAAACCAAAACAAGCTAAAACATCTTCTAGTACTTCAGGATGGAACTATAATAAAACAGTTGGAAGTTACTGGAAATCAGAAAAAGCAACATTTACTTGTACAACACCTAAAGGTATTGTTACAAGATATAATTCACCTACTTTAAGAAGTCAAAAAGCAGGGTTGTTAAGAAAAGGTCAATCAGTTGTATATGATGAGATTGCAATTAAAGATGGATACGTATGGATTCATTGGATTGCTTATGGTGGAAGTCATATATGGATGCCTGTAAGACCTTGTGTTGGTACGCCTCCTAATCATAAAGTAAAAGGTGCATGGGGAACATTTAAATAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7A7gP) rather than this protein.
PDB ID
7A7gP
Method AlphaFoldv2
Resolution 83.66
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50