Protein

Protein accession
A0AAF0A642 [UniProt]
Representative
74eYR
Source
UniProt (cluster: phalp2_1053)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MINNSNRVDAIIIHCSATREGQDIGAKEIDAMHKQRGFNGIGYHYVIRLDGTIEKGRNETAVGAHCNTKGFSRESYNRHSIGICYVGGLDKTGRAKDTRTPEQKEALIDLINDICKRYPIVELLGHRDTSPDKNGSGEVEPFEFIKMCPCYDVRAEYGTFMKNVVITP
Physico‐chemical
properties
protein length:168 AA
molecular weight:18795,1 Da
isoelectric point:7,00
hydropathy:-0,54
Representative Protein Details
Accession
74eYR
Protein name
74eYR
Sequence length
268 AA
Molecular weight
28686,27770 Da
Isoelectric point
7,18476
Sequence
MNVAEIQRALLARGYDLGQSGQARDGADGEFGPKTRAAVLAELARHPGYLAPGAAPSAWSDSRLIVAAEQLILCLAGIPTGTVDGIAGALTRIAREAWAKRVAGGTPVLPARPVLRPVHTLVWHCTATPEGKEFPVAQIDAMHRARGFAQIGYHKLVHLDGTVSEGRPEHLAGAHVEGHNTGTLGYAYVGGVDANGRPKDTRTAEQMQAMIRLTRETIARYRLRAVMGHRDFSPDRDGDGIVEPHEWVKVCPCFNAIAEYGALLNEAA
Other Proteins in cluster: phalp2_1053
Total (incl. this protein): 26 Avg length: 180,2 Avg pI: 7,52
Protein ID Length (AA) pI
74eYR 268 7,18476
23b92 245 9,51665
6x2N6 248 10,09029
7ylIh 232 9,41962
A0A7U3MWA9 171 6,19922
A0AAE9UWA4 168 7,63350
A0AAE9UYL3 168 6,99542
A0AAE9V1E3 168 7,63350
A0AAE9V1K7 168 7,63350
A0AAF0A756 168 6,99542
A0AAF0A8M9 168 6,99628
A0A8S5MLH8 173 7,64197
A0A8S5MTF5 164 5,69944
A0A8S5PXC2 165 6,99332
A0A8S5QEP7 164 6,19508
A0A8S5TX74 163 5,94407
A0A9Y1HT69 171 5,94873
A0A9Y1MRT0 168 8,18563
A0AAE9UWK9 168 8,18557
A0AAE9UX12 168 7,63049
A0AAE9UYA9 168 8,18563
A0AAE9V0Z0 168 8,18557
A0AAF0A6W0 168 6,99679
A0AAF0A7F2 168 8,18557
A0AAF0A806 168 8,18557
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_23766
13ajC
 287 38,1% 202 7.880E-54
2 phalp2_23565
7vNZv
 154 33,6% 217 1.804E-34
3 phalp2_18379
5Eq5Q
 10 29,9% 204 3.134E-23
4 phalp2_27876
8EUCV
 18 25,0% 212 1.092E-17

Domains

Domains [InterPro]
Unannotated
Ami2
Representative sequence (used for alignment): 74eYR (268 AA)
Member sequence: A0AAF0A642 (168 AA)
1 268 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacteroides phage BT638P6
[NCBI] 		2968599 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OP172724 [NCBI]
CDS location
range 1488 -> 1994
strand -
CDS
ATGATAAACAACAGCAATAGGGTAGACGCTATAATTATCCATTGTAGCGCTACTAGAGAAGGGCAGGACATCGGAGCTAAAGAGATTGACGCGATGCACAAACAGCGCGGATTCAACGGAATAGGCTATCACTATGTCATTCGCCTGGACGGGACGATCGAGAAGGGCAGGAACGAAACGGCGGTAGGCGCACACTGTAATACGAAAGGGTTCAGCAGGGAGTCGTATAACCGTCACTCTATCGGTATCTGCTATGTCGGAGGACTGGATAAGACCGGGAGGGCAAAGGATACCCGCACACCGGAGCAGAAGGAGGCGTTGATAGACTTAATCAACGACATCTGTAAACGCTACCCGATCGTGGAGCTACTGGGTCACAGGGATACGTCACCCGACAAGAACGGAAGCGGCGAAGTCGAACCATTCGAATTCATTAAGATGTGCCCCTGCTATGATGTACGCGCCGAGTACGGCACATTCATGAAGAACGTAGTGATAACACCATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008270 zinc ion binding molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (74eYR) rather than this protein.
PDB ID
74eYR
Method AlphaFoldv2
Resolution 90.72
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50