Protein

Protein accession
A0AAE9YG27 [UniProt]
Representative
8Jtqs
Source
UniProt (cluster: phalp2_6450)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVKIKQDLVSAAIIERESNGYGNPKTYIVIHETANTNVGADAQSHANLQKNGNSRDASWHYTVDDKEAVQSFPDSVKCWHSGSSYNSNSIGIEICVNPDGNFKQAVKNAAELTKSLMNKYGIPKSHVITHREASGWKDCPHNLRSGAKGVTWDDFLKMLDGKASIAPAPAKPAPSKSSGSPDFNTNSVVDFLSSVGVDSSLANRKKLASKYGISGYEGTAAQNSKLLDKLKADYKKSGSSKPSTKGDQKTNSIVEYLDSIGVDSSFANRKKLADKYGISGYTGTASQNTKLLDKMRSGAAPKPKGTKGDQKTTSLVDYLKSIGEDSSFANRSKLAAKYGVKNYEGTSSQNTQLLKKIRGR
Physico‐chemical
properties
protein length:360 AA
molecular weight:38762,9 Da
isoelectric point:9,54
hydropathy:-0,71
Representative Protein Details
Accession
8Jtqs
Protein name
8Jtqs
Sequence length
288 AA
Molecular weight
31868,80840 Da
Isoelectric point
9,79708
Sequence
MVQIKKQLVSSRKNTYTGINGRKYITIHETDNTNKGANAQAHANLQSRGNSRSASWHWTVDDKEAIQSFPHTVRCWAAGDGEGNGNFNSIHIEICVNSDGDFNKAVENAAALTRMIMEQENIPLSNVVQHNHWSGKNCPRNLRSGSKGMTWNDFLNMVVGKKVDTPKKEVKPAQTKNKTNIIVDGKWGSETTKALQKALGTVVDGVISSQPRNHVTAAIYDGITWGTKGSMMVRALQKKVGAKVNGKLDSETIRKLQKYLGTPIDGKISRPESLMVKELQRRLNEGTF
Other Proteins in cluster: phalp2_6450
Total (incl. this protein): 137 Avg length: 296,4 Avg pI: 8,76
Protein ID Length (AA) pI
8Jtqs 288 9,79708
13D1N 301 5,36034
1HKgp 222 9,51684
1abKI 314 9,48022
1bEUQ 317 9,25897
1bSoI 317 9,18463
1bUEL 310 9,51665
1bXZM 288 9,33007
1bt46 314 9,54159
1bxc2 316 9,53856
1c1Fc 260 9,39970
1c82q 311 9,50601
1c9dg 314 9,46894
1ccl6 262 9,01605
1cgJD 322 9,47326
1cjbK 314 9,42929
1jKUi 314 9,49421
1jKXj 314 9,27121
1m0Sz 272 9,27495
1m1bj 313 9,57028
1mJuP 262 9,01605
1mYYX 305 4,77518
1msRF 314 9,49434
1mssI 321 9,51645
1o4li 295 4,75523
1oj1t 253 4,76523
2G569 288 9,78960
2G7og 304 9,61296
2G8Rm 304 9,66454
2Yv0L 293 9,68929
2Yv9U 295 9,83763
2dhDg 306 9,09676
2jYtB 259 9,11855
3BTCw 322 9,35599
3BTEB 312 9,70354
3BTEe 314 9,37636
3O4Y 317 6,27550
3Va2P 318 5,80630
3e4v5 298 7,05221
3jHPe 321 5,13901
3jTeL 310 9,62302
3swgp 438 5,00651
44XZ9 318 5,83085
4LA4i 268 9,00490
4LDUj 279 9,33691
4LhnR 270 9,16871
4Lo6e 245 9,15665
4Lvw6 255 9,34864
4LwGH 270 9,11591
4Lza4 272 9,47493
4MKGt 256 9,43664
4MPUJ 274 9,40054
4lyka 312 6,10044
5NQOk 261 9,42097
5O2IY 279 6,83048
5ObGY 313 9,50485
5Snjr 316 9,55907
5hPUR 283 8,74567
5hS73 300 9,61689
5hSas 300 9,68691
5imGi 259 6,66457
5tDlr 291 9,38706
69MGK 285 9,45263
69Vgq 262 9,01605
6UKKT 288 9,39938
6bsyw 280 6,15461
6dEYk 196 4,68015
72xff 249 9,22699
752ah 259 9,01618
752bH 259 9,02662
758Vj 245 9,12713
75CZc 232 9,51871
75OSk 359 9,44534
76gOX 284 9,56461
76hD7 280 9,59620
77iWW 325 9,83983
79xRV 316 9,70283
7Xvjf 314 9,46894
7ZGGm 283 4,72198
7aZRU 222 9,63817
7cKxe 222 9,65010
7coba 291 6,93699
7cype 293 9,44289
7drhg 226 9,65055
7fJ3n 273 5,86001
7fLPR 309 5,57672
7lBrW 250 9,07233
7lDUy 259 9,01605
7lE4p 314 9,41872
7mxKf 289 7,65135
7ouii 314 9,44747
7pYRe 258 8,85630
7qIpX 223 9,58260
7rMwZ 222 9,56590
7rVNF 296 6,14216
7s43h 226 9,66795
7sbAF 263 6,11721
7sxY9 226 9,60490
7sy0c 226 9,61038
7wkwS 381 9,32820
7x4ai 259 9,20791
7x4b4 259 9,11855
7xE51 293 9,50672
7zjgr 292 9,23917
8138 234 9,50092
86j9q 314 9,40847
87Ws4 314 9,53889
89VWK 310 9,86858
8czdA 327 4,79581
8obEn 314 9,43683
8owng 285 6,04263
8tTNL 269 9,37056
9wCP 278 6,79143
OGJx 276 4,82162
aJoB 289 9,32285
aQSb 289 9,68671
cFyJ 253 9,36418
A0A1L2JY70 288 9,79708
R4JGH8 364 9,75447
A0A217ER07 360 9,55855
A0A2P1JU23 330 9,64146
A0A7T8C4M4 364 9,76491
A0A9X9E1J8 360 9,50014
A0AAE7S5U2 364 9,75447
A0AAE7SCR8 364 9,75447
A0AAE9YE35 360 9,53876
A0AAE9YEG1 361 9,46894
A0AAE9YKD0 360 9,50949
A0AAE9YM67 360 9,53876
A0AAN0N6V3 364 9,75447
A0AAX4NUD1 360 9,60194
A0AAX4Q5N7 364 9,75447
A0AAX4Q5P0 364 9,75447
A0AAX4Q7D7 364 9,75447
A0AAX4Q8J7 364 9,75447
D6QWL8 329 9,68716
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_24835
7sl6i
 39 44,5% 321 1.693E-79
2 phalp2_7826
aMK
 29 64,6% 181 3.844E-69
3 phalp2_29544
3KzTO
 225 43,7% 231 3.734E-63
4 phalp2_4901
5Zr0m
 117 31,2% 358 4.090E-61
5 phalp2_17898
EGF4
 130 33,7% 305 1.351E-54
6 phalp2_15067
7vQ2g
 6 38,7% 209 2.054E-47
7 phalp2_416
7vQHy
 10 34,4% 308 6.317E-46
8 phalp2_33474
7qPh3
 3 41,2% 177 2.995E-45
9 phalp2_14020
81gAb
 48 27,1% 195 2.995E-45
10 phalp2_27725
6UNCq
 90 34,7% 242 4.089E-45

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 8Jtqs (288 AA)
Member sequence: A0AAE9YG27 (360 AA)
1 288 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage vB_BsuM-Goe20
[NCBI] 		3026982 Herelleviridae > Grisebachstrassevirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OM728301 [NCBI]
CDS location
range 20855 -> 21937
strand -
CDS
ATGGTTAAGATTAAGCAGGACCTCGTTTCCGCAGCTATTATCGAAAGAGAGTCTAATGGGTATGGAAACCCTAAAACTTACATCGTAATCCACGAGACAGCTAACACTAACGTAGGCGCAGACGCCCAAAGCCACGCTAACCTTCAAAAGAACGGGAATTCTCGTGACGCTTCTTGGCACTACACTGTAGATGATAAAGAAGCAGTACAGTCTTTCCCAGATAGTGTTAAATGTTGGCATAGTGGAAGTTCTTACAATAGTAACTCTATTGGTATTGAAATCTGCGTTAACCCTGATGGTAACTTCAAACAAGCTGTTAAAAATGCTGCTGAGCTTACAAAATCCCTGATGAATAAGTACGGTATTCCTAAATCTCATGTTATCACTCACCGGGAAGCTAGCGGGTGGAAAGACTGCCCACATAATTTGCGTAGTGGTGCAAAAGGGGTTACTTGGGATGACTTCTTAAAAATGCTTGATGGTAAAGCTTCTATTGCTCCAGCACCTGCTAAACCTGCACCATCCAAGTCTTCAGGCTCTCCAGACTTCAACACTAACAGCGTTGTTGATTTCCTAAGCTCAGTGGGTGTCGACTCTAGCCTTGCTAACCGCAAAAAACTTGCTAGCAAGTATGGAATCTCAGGTTACGAAGGCACTGCTGCACAGAACTCTAAGTTGCTGGATAAGCTCAAAGCAGATTATAAGAAATCAGGAAGTTCCAAGCCTTCAACGAAAGGGGACCAAAAAACTAACAGTATTGTTGAGTACTTGGACTCTATTGGTGTGGACTCCAGCTTTGCGAACCGTAAGAAACTTGCGGATAAGTATGGTATTTCCGGGTATACAGGTACTGCTTCGCAGAATACAAAACTTCTCGATAAGATGCGTAGTGGCGCAGCTCCGAAGCCCAAAGGCACTAAAGGAGATCAGAAGACCACTAGTCTTGTAGATTACCTTAAGTCTATTGGAGAAGACTCTAGCTTCGCCAACCGTAGTAAACTAGCTGCTAAGTACGGAGTTAAGAATTATGAAGGAACGTCTTCACAGAATACACAGCTCCTCAAGAAAATTCGAGGACGTTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8Jtqs) rather than this protein.
PDB ID
8Jtqs
Method AlphaFoldv2
Resolution 87.06
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50