Protein

Protein accession
A0AAE9K5G9 [UniProt]
Representative
1rrTr
Source
UniProt (cluster: phalp2_23855)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVKITKDFIPVGHNNRPGYVMEPAYITVHNTANTQKGANAASHARYAKNPGTPTSWHFTVDEKEIYQHLPLNENGWHAGDGNRGTGNRKSIGIEICENSDGDFEKAVANAQWLIKKLMKEQGISLANVVPHQHWSGKYCPRKLLDRWDSFKAGISGASSTPSKTVTKPGATYTVKKGDTLSEIAQEIGVSMANLQKWNNIADPNKIKVGQVLKLTGSSSSSKPSSSGKNYVYLPASADSWRIYPTNKAPVKGNEIDFLRPKKFGGLKYKILANPQKDVYTIKTDQFGKVNIFAAKSTGATVR
Physico‐chemical
properties
protein length:302 AA
molecular weight:33120,2 Da
isoelectric point:9,73
hydropathy:-0,61
Representative Protein Details
Accession
1rrTr
Protein name
1rrTr
Sequence length
465 AA
Molecular weight
50542,89470 Da
Isoelectric point
5,17112
Sequence
MSYTRNSPIQGTGVASAEAIQAWFAANAARGASWVGLPVAPIPADLGVSIVRSAGRYGFNSDEGAAQIAHETAFGQSRIFRDKLNPAGIGAENDDPYRKALTYTTVGDGCDAWAAHLAGYVLGDGPWNALSPRYAIVKANGWAGIVRVLADLEQRWAWTPQAKYDATPLDQRYGAKVAALANDLVDFANNQTWEPPMADIPIIQMLLPVDASNTPRRSMTPLYITIHETANPAKGANAVMHGRWLLNLANDGADEPSWHYTVDDHQIVQHMPINRAGWHAGDGGNGTGNMESIGIELCVNSDGDIQKTRDNAAWLVRKLRSETGIPADRVVQHNHWSGKDCPRLLRQSGWAAFIASIQDAPEPTPEQPVDKWVIPDGPNKGAGFQQGFRAWLETQARARFPDDLNAGALAITGYPEADEYGADDGCSYQVCQRMTLRWMPNTAPPWDIVPLLRDEPIPPPKGSES
Other Proteins in cluster: phalp2_23855
Total (incl. this protein): 15 Avg length: 434,5 Avg pI: 6,44
Protein ID Length (AA) pI
1rrTr 465 5,17112
1kVl0 461 5,53898
1rpiQ 456 5,47589
1zFqB 453 5,32334
3OP9d 448 6,36218
4fLR9 457 6,92898
5FnWN 466 5,87774
5uopP 364 8,92476
6Rep1 457 6,46540
6Vw9q 485 5,77310
7CA1D 481 4,94195
7CCLc 475 4,99697
iM3V 447 5,47913
Q9ZXD7 300 9,65538
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_39653
6POYF
 2 30,3% 352 3.102E-52
2 phalp2_30194
3UcKC
 10 28,1% 419 4.746E-41
3 phalp2_1662
8jjNM
 6 24,5% 457 5.247E-40
4 phalp2_20140
3tObF
 100 27,9% 361 2.276E-36
5 phalp2_19797
4X6CD
 7 20,8% 441 5.385E-22
6 phalp2_3886
6Rlv5
 7 21,2% 513 2.073E-17

Domains

Domains [InterPro]
GLUCO
Ami2
Unannotated
Representative sequence (used for alignment): 1rrTr (465 AA)
Member sequence: A0AAE9K5G9 (302 AA)
1 465 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF01832

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage rho14
[NCBI] 		2917162 Spizizenvirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OM236514 [NCBI]
CDS location
range 20614 -> 21522
strand +
CDS
ATGGTGAAAATCACAAAAGACTTTATTCCAGTAGGACACAATAACAGACCGGGATACGTAATGGAACCGGCATACATCACAGTTCACAACACGGCGAACACGCAAAAGGGGGCAAACGCTGCCAGTCATGCTCGCTATGCTAAAAACCCGGGAACACCTACCAGTTGGCATTTTACAGTTGACGAAAAAGAGATTTATCAACACCTACCCTTGAATGAAAACGGTTGGCACGCGGGCGACGGAAACCGCGGAACCGGGAACCGGAAATCTATCGGCATTGAAATTTGTGAGAATAGCGATGGGGATTTTGAAAAAGCCGTGGCGAATGCTCAATGGCTGATCAAAAAGCTCATGAAGGAGCAGGGCATTTCCCTTGCAAACGTGGTCCCTCACCAGCACTGGTCCGGCAAGTATTGTCCGCGCAAGCTGCTTGATCGATGGGACTCCTTTAAAGCAGGTATCAGCGGGGCATCGTCAACGCCGTCGAAAACAGTAACAAAGCCCGGGGCGACCTACACCGTTAAGAAAGGTGATACTTTGTCCGAAATTGCACAGGAGATAGGCGTAAGCATGGCAAACCTCCAGAAATGGAATAACATCGCTGACCCGAATAAGATTAAAGTGGGCCAAGTACTGAAGCTCACGGGATCATCCAGCTCTTCTAAGCCGTCATCCAGTGGCAAGAATTACGTTTATCTTCCGGCTTCTGCCGACTCATGGCGTATCTATCCGACAAACAAAGCGCCGGTTAAGGGGAACGAGATCGACTTCCTCCGTCCGAAAAAATTCGGCGGCCTTAAATACAAAATCCTTGCAAACCCGCAAAAGGACGTTTACACGATCAAGACGGATCAGTTCGGAAAGGTAAATATTTTTGCGGCTAAGTCCACAGGCGCAACAGTAAGGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (1rrTr) rather than this protein.
PDB ID
1rrTr
Method AlphaFoldv2
Resolution 87.39
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50