Protein

Protein accession
A0AAE9CEC5 [UniProt]
Representative
7z7dc
Source
UniProt (cluster: phalp2_13879)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAISVRQKLVDSSKYSLKCPYSMTAEYITIHNTYNDASANNEVQYMITNSNATSFHYAIDDFEVVQGIPTNRNAWHCGDGNGNGNRKSIGVEICYSMSGGDRYRKAEALAIKFVAQLLRERGWGIDRVKKHQDWSGKYCPHRILDEGRWQSVLNAIKAELNGGGSTGGGTTQPPVDNSTGVVRVTADVLNLRDQPSTNGSIVGKIYKGEDYKFWGVSNGWYALGGNQWASGQYLQVISGGTSQPKPVTGTAYITGYNVNMRTGAGTGYSVIRQLNAPEAYKVWGMKDGWLNLGGDQWIKNDSSFVRFVQD
Physico‐chemical
properties
protein length:310 AA
molecular weight:34125,7 Da
isoelectric point:8,86
hydropathy:-0,52
Representative Protein Details
Accession
7z7dc
Protein name
7z7dc
Sequence length
233 AA
Molecular weight
25786,89950 Da
Isoelectric point
9,16252
Sequence
MAYELVNRLIPPSLYSLKAPYAMTPEYITIHNTYNDATAANEIAYMTRNTAATSYHVAIDDKQAIQAVPFGRNAWHAGDGQGAGNRKSIGIEICYSKSGGKRYYDAEDNAVIYVAHVLKQYGWGVDRIRWHRDWSGKLCPHRMIAEGRLDSFKERVQAELSGKPGSNPVAKSEGGARMFKPTSATLNDAYAKFLQDAIAGGHLNAKWLADFKAGKLSLDDALALFVIESQRKK
Other Proteins in cluster: phalp2_13879
Total (incl. this protein): 255 Avg length: 257,3 Avg pI: 7,95
Protein ID Length (AA) pI
7z7dc 233 9,16252
13bOR 249 5,39404
1FkkM 297 9,41221
1GzVP 210 9,22132
1NS8t 236 6,66218
1PViP 260 8,76688
1abEb 244 8,93585
1bIG1 202 6,09504
1bQIl 236 7,67414
1bZ9q 235 8,56122
1bt8x 255 6,06594
1byEq 238 7,67846
1bzFH 236 9,10753
1c0dI 227 9,25806
1c0tv 238 8,90484
1c5ll 215 7,75923
1cgkG 235 8,73832
1cnLs 227 7,69693
1dMdM 237 9,14125
1dMwg 226 8,93701
1iZuS 285 9,85781
1jKFb 227 9,19056
1jQ7z 227 9,16581
1knAU 220 9,26367
1lYHa 201 8,36904
1mHj7 238 8,78312
1mI1q 236 8,78280
1mIAa 232 9,27096
1mIwn 236 8,27911
1mt42 238 8,77307
1mxLk 237 8,79286
1nWom 324 8,17686
1qvg1 273 9,43993
1rrBg 249 8,82638
2Ckla 227 8,20768
2G8eJ 309 9,68194
2UYUe 234 6,95393
2XKyd 237 5,87683
2XKzR 236 5,47555
2e4Cl 239 8,76385
2e4sc 236 8,58147
2ejxT 238 8,30258
2g1ED 236 8,94668
2g2Xe 238 8,59836
2kCuu 227 8,60829
2mpoX 211 8,92534
2ocxP 241 8,57199
37MYw 218 9,08812
39BGb 227 9,10959
3ATFk 366 9,93182
3X679 256 6,55151
3k76a 321 8,39980
3kSnf 266 5,21369
3kgI5 321 8,41192
3lyyH 290 8,53370
3lzsm 325 7,08034
3sy7i 324 7,66840
3tX47 237 4,99174
3uGjI 290 8,79898
3vUIx 266 5,20886
3vtGj 266 5,64175
3wQvw 236 9,54056
3wiL9 267 5,12070
3yIdX 266 5,13088
4GM9n 302 9,51922
4LkmV 195 9,23440
4SvdH 224 8,94894
4g23E 275 5,65590
5N6xN 266 5,21369
5NGmZ 260 5,01612
5NpdV 269 8,61086
5PA5T 236 5,07330
5QBWI 260 5,01612
5QqnC 264 5,26428
5RQQP 235 9,46630
5Vut8 260 5,26422
5W566 324 6,71129
5XiQa 260 5,00867
5Xu8H 266 5,06046
5YCXj 266 5,28133
5Z9XL 255 8,51358
5ZQHg 269 8,11775
60efu 260 4,90324
62LGD 266 5,11644
649B2 266 5,08012
67KL1 316 8,12767
68IbX 325 7,08029
68giU 260 4,91085
69dC8 266 5,30827
6Rgo7 247 6,66474
6Ri5l 242 6,01450
6TeLR 258 9,38017
6dXxt 266 5,26184
6eI3v 266 5,18965
6fIgf 266 5,12338
6h5Rv 266 5,14463
6h8ew 269 7,58359
6iQRv 235 9,41401
6mYr4 266 5,20858
6o7eD 266 5,51880
6oTMD 260 5,10325
6omG4 260 5,17322
6pb2K 269 7,58359
6q6FN 260 4,94661
6qGjK 266 5,55359
6qT1R 260 4,91841
6qsNt 220 5,87661
6r4Dn 255 5,34567
6sOBl 260 5,00993
6ssPf 321 8,60648
6tAYt 260 4,94825
6tOwC 266 5,62066
6uNSK 260 5,12519
6vhrG 266 5,06756
6vnd1 215 9,28798
6wLtd 266 5,73155
71ETD 258 9,35535
72mbl 352 9,47829
72nvz 238 8,90465
72rgz 260 7,66647
72xec 224 8,55091
72y6h 233 9,15337
72yIn 289 10,02098
74cph 251 8,54981
75uUt 278 9,29945
75vij 279 9,39177
75vzL 279 9,40653
75xPG 281 9,40673
78QUq 238 8,95816
78QVy 236 8,84688
79KPB 236 8,91477
7BBhX 214 5,95493
7BF1E 204 8,94919
7BI7u 255 8,51345
7QFYi 242 7,01515
7RlxA 291 9,21977
7Rprz 201 9,40873
7WECc 266 5,80584
7XCMs 266 5,07484
7c8RM 236 9,11842
7caR4 234 7,60929
7cszO 302 9,13822
7ctRr 228 9,03365
7dG0Z 235 8,22057
7dTgT 235 8,84727
7dTi7 238 9,11855
7dWpp 236 9,71095
7dewu 231 9,37307
7dizX 237 9,09689
7e9ds 242 7,01515
7ff9r 289 10,09989
7g4U8 279 9,32743
7gmjL 279 9,35747
7hwW4 242 7,01924
7iCa0 279 9,25432
7jTAW 237 8,19936
7jioi 251 8,48947
7jmCZ 278 9,26883
7jmSm 279 9,35773
7kQH1 282 9,54824
7lty5 251 8,54981
7luCL 250 8,54962
7mxGu 229 8,25983
7nIaY 237 9,02662
7ofVU 289 10,05857
7oqY3 244 9,18450
7or1Y 237 8,26873
7ou8K 290 8,48767
7ovqe 242 7,72944
7p77E 240 7,69398
7pZlY 252 9,35309
7pZmi 234 9,21487
7pln5 238 9,01598
7ploW 236 9,02630
7pqAm 232 9,43000
7pqCP 227 9,25774
7pqFa 227 9,38732
7pqvP 227 9,16574
7pqyw 231 8,74754
7qvw8 226 9,20533
7r79Y 238 7,63924
7rkop 234 7,65271
7rlAQ 234 6,59249
7rmEn 205 7,63873
7s3Yu 239 8,76372
7tHAz 238 9,03739
7tHJD 227 9,24485
7tddT 216 9,46204
7vFlo 247 8,82168
7wEwi 251 9,36431
7wEyW 235 8,90471
7wEzL 205 9,33136
7wt3m 228 9,24923
7x5CM 236 8,76378
7xQFY 255 8,50830
7xRsK 211 9,62850
7ycF6 282 9,55829
7ycOT 282 9,57944
7z5MR 365 10,16217
7zHyC 227 9,27134
80cRP 209 8,70357
83V6b 266 5,37205
83wYJ 246 9,30016
84pEe 266 5,14719
85VSh 219 8,73813
87Rf9 214 9,20797
87pP6 260 7,67181
87zLY 272 5,12753
88CMB 246 9,23453
88omP 266 5,07358
8K7vn 233 8,72955
8KbsQ 212 9,58356
8Quf 217 9,48506
8a4ub 259 8,24069
8eB4s 316 8,10814
8fsGX 214 8,91516
8nEmi 239 9,04822
8q0HW 262 8,55007
8rx7z 319 8,58766
8tFWX 325 8,17680
8tFdE 325 7,08097
8vsPl 266 5,14463
IoWr 257 7,64879
VUtV 251 6,37474
ZjUT 214 8,76340
b88e 226 8,72014
bS6 237 7,62151
gpqS 235 6,41084
i2fH 225 6,36133
oaJu 236 5,59582
ohX4 236 6,20332
A0A143FL92 275 8,63704
A0A143FL68 275 8,83509
A0A345AXR0 276 8,63704
A0A2K9V6C1 277 8,63704
U5J9E2 313 8,74683
A0A218KC88 311 8,72846
G9B1A2 276 8,83509
A0A1I9S548 276 8,63704
A0A3Q9R7E9 307 8,88382
A0A6H0X6E1 313 8,73767
A0A7U3NKG8 311 8,23779
A9QTG2 311 9,18966
A0A9E7PHJ2 311 8,73742
A0A9E7PHL7 311 8,73742
A0A9E7PI28 311 8,86455
A0A9E7TI68 316 8,52480
A0A9E7TJ28 313 8,74683
A0AAE8YRL6 313 8,74683
A0AAE8YYL2 313 8,74683
A0AAE9CAX9 313 8,73761
A0AAE9CEL3 312 8,90407
A0AB39C715 313 8,73761
D6QWL7 307 9,14982
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_5182
3wjGA
 273 56,4% 170 5.158E-85
2 phalp2_16558
7rWDg
 20 68,2% 167 1.679E-80
3 phalp2_24718
6w5Af
 15 49,1% 187 3.910E-79
4 phalp2_37301
7Ya12
 3 50,8% 169 7.684E-70
5 phalp2_38408
13xIA
 10 46,8% 173 1.303E-68
6 phalp2_25038
13C0m
 57 46,7% 152 1.200E-61
7 phalp2_13869
7vyfo
 1 37,8% 201 7.158E-57
8 phalp2_18793
1bS71
 24 42,8% 161 5.756E-49
9 phalp2_39055
7ordh
 68 49,0% 153 1.474E-48
10 phalp2_29544
3KzTO
 225 42,1% 173 2.760E-48

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 7z7dc (233 AA)
Member sequence: A0AAE9CEC5 (310 AA)
1 233 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage vB_BanS_Sophrita
[NCBI] 		2894790 Sophritavirus > Sophritavirus sophrita
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OK499991 [NCBI]
CDS location
range 103493 -> 104425
strand -
CDS
ATGGCTATTTCAGTAAGACAAAAATTGGTGGATTCAAGTAAGTATTCTTTAAAGTGTCCTTACTCTATGACAGCAGAGTACATTACAATCCACAACACGTACAATGATGCAAGTGCAAATAATGAGGTTCAATACATGATTACTAATAGTAATGCTACTTCATTCCACTATGCAATTGATGATTTCGAAGTTGTACAAGGTATCCCAACAAACCGTAATGCTTGGCATTGTGGAGATGGAAACGGTAATGGTAACCGTAAATCTATCGGTGTTGAAATCTGTTACTCTATGAGTGGTGGCGACAGATATCGTAAAGCAGAAGCTTTAGCTATCAAATTCGTAGCACAACTTTTAAGAGAACGTGGATGGGGCATTGATAGAGTAAAAAAACACCAAGACTGGTCTGGTAAATATTGTCCACACCGTATCCTTGATGAAGGACGTTGGCAATCAGTTCTTAATGCTATCAAAGCAGAACTTAATGGTGGAGGTTCTACAGGTGGAGGAACAACTCAACCACCAGTTGATAATTCTACAGGTGTAGTTCGAGTAACAGCAGACGTTCTTAACTTGCGTGACCAACCATCAACTAATGGAAGCATTGTTGGTAAAATCTACAAAGGTGAAGATTACAAGTTCTGGGGTGTAAGTAATGGATGGTATGCACTTGGTGGAAATCAATGGGCTTCTGGTCAATACTTACAAGTAATCAGTGGAGGAACATCACAACCAAAACCAGTAACAGGTACAGCATATATCACTGGATATAATGTTAATATGCGTACAGGTGCAGGAACAGGATACTCTGTAATCCGTCAATTAAATGCACCAGAAGCGTACAAAGTTTGGGGAATGAAAGACGGTTGGCTTAACCTTGGTGGAGACCAATGGATTAAGAACGATTCTTCATTCGTTAGATTTGTACAAGACTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7z7dc) rather than this protein.
PDB ID
7z7dc
Method AlphaFoldv2
Resolution 92.10
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50