Protein

Protein accession
A0AAE7WZ60 [UniProt]
Representative
2Yvfy
Source
UniProt (cluster: phalp2_8874)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MVKVRKELVSENVIENASYGYGNPKTYIVIHETANTAKGANAASHAKLQKNGNSRSASWHYTVDEKEAIQSFPDNVKCWHSGGSYNSNSIGIEICVNSDGDFKQAVKNAAELTKSLMNKYGIPKSHVITHREASGWKDCPHNLRSGAKGITWNDFLKMLDGKASVVTEPAKSTPAKSTPSKSSGSPNFDTNSIVDFMNSVGLDSSFNNRKKYADKYGIKGYEGTASQNAKLLEKLKVVYKSIPSSKPKTSKGDQKTNSIVEYLDSIGVDSSFSNRSKLAAANGIKGYKGTESQNLELLKKLRGGGKTSAPKKAKGDQKTTSLVDYLKSIGESSDFKNRAKLAAAKGIKNYEGTASQNTQLLKKLRGH
Physico‐chemical
properties
protein length:367 AA
molecular weight:39774,3 Da
isoelectric point:9,68
hydropathy:-0,73
Representative Protein Details
Accession
2Yvfy
Protein name
2Yvfy
Sequence length
155 AA
Molecular weight
17253,93350 Da
Isoelectric point
9,19669
Sequence
LSITIKRQIVSDSIARQRTYGNGNQKRTITVHQTGNTSRGANAQAHANIQSRLNPRQASWHYSVDDKEIIQSYEDSAQCWHAGDGRGPGNLHSVSIEICINSDGDYVKAVENGAKLVKHLIEKYGLSIGDVKQHYDWSRKNCPAQIRAGKDGINW
Other Proteins in cluster: phalp2_8874
Total (incl. this protein): 25 Avg length: 229,8 Avg pI: 8,09
Protein ID Length (AA) pI
2Yvfy 155 9,19669
11xfX 138 6,48393
13DRe 219 7,15446
2VFUR 179 8,41243
3bHVu 167 5,12417
3eVKp 166 6,04439
4NMCg 127 9,17148
4Z9uO 171 6,59192
4Zlru 238 9,65300
62aOx 229 8,28511
6sl0H 184 9,13106
70wJo 179 6,78614
7Osks 179 6,78614
7Ylqf 248 5,74934
7lBwO 198 9,30029
7pdlc 225 8,12110
7sdMt 218 7,69773
7sdNI 219 6,82474
861wv 225 8,41217
8kVVP 242 9,06363
A0AAE7WZA9 367 9,65951
A0AAE7X063 369 9,66995
A0AAE7X0A4 369 9,70270
A0AAE8BNK5 367 9,65951
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_7826
aMK
 29 59,4% 153 1.667E-67
2 phalp2_5052
1q1Q3
 64 34,3% 134 2.803E-39
3 phalp2_24292
3k9WX
 42 42,8% 147 1.688E-37
4 phalp2_30401
4NIg7
 17 50,9% 104 2.424E-31
5 phalp2_17726
7f6JX
 68 41,1% 119 5.114E-29
6 phalp2_25038
13C0m
 57 35,8% 148 8.685E-28
7 phalp2_3793
5X3JD
 59 38,3% 133 3.057E-27
8 phalp2_29669
8sfID
 1005 32,5% 160 1.473E-26
9 phalp2_25288
86peP
 187 28,3% 127 7.099E-26
10 phalp2_19086
1mxL7
 3 40,5% 106 1.085E-23

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 2Yvfy (155 AA)
Member sequence: A0AAE7WZ60 (367 AA)
1 155 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage 035JT004
[NCBI] 		2869564 Herelleviridae > Nitunavirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MZ501262 [NCBI]
CDS location
range 138017 -> 139120
strand +
CDS
ATGGTAAAAGTTAGAAAAGAACTCGTTTCCGAAAATGTTATCGAGAATGCATCCTATGGCTACGGTAACCCTAAAACTTACATTGTAATCCACGAGACAGCTAATACTGCAAAAGGTGCTAACGCAGCAAGTCACGCTAAATTACAGAAGAACGGCAACTCCCGCAGTGCATCTTGGCACTATACTGTAGACGAGAAAGAAGCCATACAGTCATTTCCAGACAACGTTAAGTGTTGGCACAGCGGAGGCTCTTACAATAGCAACTCTATTGGTATCGAAATCTGCGTAAATTCAGACGGTGATTTTAAGCAAGCTGTTAAAAATGCTGCCGAGCTTACAAAGTCTCTGATGAACAAATATGGCATTCCTAAATCTCATGTCATCACACACCGAGAAGCTAGTGGATGGAAAGACTGCCCGCATAACTTACGTAGCGGCGCCAAAGGCATCACTTGGAACGATTTCTTGAAGATGCTCGATGGCAAAGCTTCTGTTGTTACTGAGCCTGCAAAATCAACTCCAGCGAAGTCTACACCATCTAAGTCTTCAGGATCACCAAACTTTGACACAAACAGCATCGTAGACTTCATGAACTCCGTAGGTCTCGATTCCAGCTTCAATAACCGTAAAAAATATGCTGACAAGTACGGAATAAAGGGATACGAAGGCACAGCGTCTCAGAATGCTAAGCTGCTTGAAAAGCTTAAAGTAGTTTATAAGAGTATTCCTAGCTCTAAACCGAAGACATCTAAAGGAGACCAAAAAACCAACAGTATTGTTGAGTATTTAGATTCTATCGGAGTAGACTCTAGCTTCTCAAACCGATCTAAGTTAGCAGCTGCAAATGGCATTAAAGGGTACAAAGGCACGGAGTCTCAGAACTTAGAACTGCTTAAGAAATTGCGTGGTGGGGGAAAAACTTCGGCTCCGAAGAAAGCCAAAGGCGATCAGAAGACAACAAGCCTAGTAGACTATCTGAAGTCTATCGGTGAATCTTCTGATTTCAAAAACCGAGCTAAACTAGCTGCTGCTAAAGGCATTAAAAACTATGAGGGCACAGCCTCCCAGAATACACAGCTGCTCAAGAAACTTAGAGGACACTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (2Yvfy) rather than this protein.
PDB ID
2Yvfy
Method AlphaFoldv2
Resolution 98.03
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50