Protein

Protein accession
A0AAE7UXS5 [UniProt]
Representative
7f6JX
Source
UniProt (cluster: phalp2_17726)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MKGGQKVMEIRKVLVDPSKYCIKCPNKMTPKYITFHNTSNDAPAENEIRYMIGNNNEVSFHVAVDDKEAVQGIPFDRNAWHCGDGNRTGNRQSIGVEICYSKSGGSRYYKAEDNAAIIIAQLMKQFCIPIGNVVPHQHWSGKYCPHRMLDEGRIPSFIERIKQAYEGEEDDMNRILQLEDWQWKQLFDNMGKAWNAGRFRDWNWMVKIENHTLTVDELVWLNNHILASGLA
Physico‐chemical
properties
protein length:231 AA
molecular weight:26662,0 Da
isoelectric point:6,71
hydropathy:-0,59
Representative Protein Details
Accession
7f6JX
Protein name
7f6JX
Sequence length
139 AA
Molecular weight
15761,59530 Da
Isoelectric point
7,69057
Sequence
MEIRKKLVVPSKYGTKCPYTMKPKYITVHNTYNDAPAENEVNYMITNNNEVSFHVAVDDKQAIQGIPWERNAWACGDGNGPGNRESISVEICYSKSGGDRYYKAENNAVDVVRQLMSMYNIPIENVRTHQSWSGKILPT
Other Proteins in cluster: phalp2_17726
Total (incl. this protein): 68 Avg length: 207,8 Avg pI: 6,48
Protein ID Length (AA) pI
7f6JX 139 7,69057
1nWyh 163 7,63572
2dgKy 124 6,80803
31EGE 216 9,10792
3GNrH 209 5,96761
41pyr 102 5,83386
65Pmn 155 7,00560
6oW8g 127 6,89425
6wgFb 120 6,40072
7Xgkt 91 6,03922
7cWWC 224 5,66414
7e37V 196 8,59391
7r0Ku 223 5,51699
7r0LJ 238 8,63910
7r0Nc 172 8,55317
7r1g8 142 6,50581
7vJMi 114 9,51922
7vyfr 161 7,11530
8c9XI 164 6,88754
8s9qN 163 5,94089
Hobu 194 6,21883
A0A2I7SC18 224 5,98022
Q2I8E6 233 8,50926
A0A0C5AMZ6 223 5,51517
A0A2I7SD00 223 5,81801
A0A2I7SCT0 223 5,98824
A0A0K2CZI0 223 5,90343
W8CZ46 233 9,42239
A0A0B5A2B2 224 5,48140
A0A2I7SDE9 224 5,66414
A0A0S2MV84 318 8,58914
A0A0K2CXY9 223 5,65891
A0A0K2CYU5 225 5,74292
B5LPS9 250 8,54962
A0A2I7SCE7 224 5,48135
A0A2I7SC76 223 5,61321
A0A345AT03 224 5,50369
A0A345AS42 225 5,35505
A0A345ARI4 225 5,97812
A0A0C5AJ66 224 5,64431
A0A345ATT8 225 6,16722
A0A3G1LEF9 233 8,71150
A0A5B7LZ61 223 5,90008
A0A5P1MAE5 233 8,93695
A0A0C5AC42 223 5,51517
A0A0C5AFD4 223 5,51517
A0A0C5AN35 223 5,48140
A0A9X9K6B1 223 5,83040
A0AA96HC34 236 8,71150
A0AAE7RE99 223 5,48140
A0AAE7REE5 224 5,50369
A0AAE7REH7 224 5,50369
A0AAE7REK8 223 5,48140
A0AAE7RG03 222 6,06565
A0AAE7RH29 223 5,50369
A0AAE7RME8 222 6,06565
A0AAE7UWC9 223 5,51517
A0AAE7UWK8 223 5,51517
A0AAE7UX66 222 6,06565
A0AAE7UX89 223 5,51517
A0AAF0BYN7 234 6,95393
A0AAX3DMQ4 223 5,83040
A0AAX3DPG9 223 5,83040
A0AAX3DPM4 224 5,83028
A0AAX3DR08 225 5,97687
A0AAX3DRA6 225 5,97687
R9VY83 224 5,74292
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_13869
7vyfo
 1 56,3% 133 1.055E-53
2 phalp2_25038
13C0m
 57 50,7% 140 2.481E-52
3 phalp2_12757
86tLk
 1 54,2% 118 4.851E-49
4 phalp2_19086
1mxL7
 3 54,3% 103 3.172E-41
5 phalp2_29544
3KzTO
 225 44,0% 143 4.350E-41
6 phalp2_33479
7swgL
 97 45,4% 143 2.634E-39
7 phalp2_18793
1bS71
 24 40,7% 140 1.277E-38
8 phalp2_32247
7tHu8
 1 56,2% 96 1.652E-34
9 phalp2_8874
2Yvfy
 25 39,8% 143 1.369E-32
10 phalp2_29669
8sfID
 1005 38,8% 152 6.843E-29

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 7f6JX (139 AA)
Member sequence: A0AAE7UXS5 (231 AA)
1 139 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Paenibacillus phage SV21
[NCBI] 		2834877 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MZ218124 [NCBI]
CDS location
range 1404 -> 2099
strand -
CDS
GTGAAGGGAGGACAGAAAGTAATGGAAATCAGAAAAGTGCTTGTAGACCCAAGCAAATACTGCATCAAATGCCCGAACAAGATGACACCGAAATATATTACGTTTCACAACACGTCCAATGATGCTCCTGCAGAAAATGAGATTCGCTATATGATTGGAAACAATAACGAAGTTTCGTTCCACGTTGCTGTGGACGATAAAGAAGCTGTTCAGGGTATTCCTTTTGATCGAAATGCCTGGCATTGCGGAGACGGGAACAGAACAGGAAACCGTCAGTCCATTGGAGTAGAAATTTGTTATTCCAAGTCTGGTGGCAGCCGCTATTACAAAGCAGAAGACAATGCGGCTATTATCATTGCTCAGCTTATGAAACAGTTTTGTATTCCTATTGGAAATGTGGTTCCGCACCAGCATTGGAGTGGTAAATACTGCCCGCACAGAATGTTAGATGAGGGAAGAATACCAAGCTTTATAGAGCGAATTAAACAAGCATATGAAGGAGAGGAAGACGACATGAATAGAATCTTACAACTGGAAGATTGGCAATGGAAACAGCTTTTTGACAACATGGGGAAAGCCTGGAATGCAGGGAGGTTTAGAGACTGGAATTGGATGGTCAAGATCGAAAACCATACCCTTACCGTTGACGAATTGGTATGGCTCAATAACCATATTTTGGCGAGTGGGCTAGCATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7f6JX) rather than this protein.
PDB ID
7f6JX
Method AlphaFoldv2
Resolution 93.43
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50