Protein

Protein accession
A0AAD1VFB9 [UniProt]
Representative
6Vxjq
Source
UniProt (cluster: phalp2_28020)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAYALASRLERAVKRAGVKYKKVSGWASRGHGTMGSIRGVVAHHTAGPASGNAPSLNVVAHGRPGLSGPLSQLFLARDGTVYLVAAGVSWHAGRVNNSNFTNSHAIGIEAEATGTSSWPAHQVDAYAKLCKALCDEFNLSTSRVRGHKEVCSPRGRKIDPNFSMNDFRAKVGGAKGGVSSGGGSTGGGSSKSYKTVSYGTTLGLYDKGDPVKEWQIEALGYKGKEADGYFGPDTVKDTKTVQKKYGLKADGLVGPKTWPLRLKEAKGKFTRPKHEKAKDAPKFPLKRGWYFGPKSGPASSVSGYYSHRSDLKRWQQRMKNRGWTIAADGLYGPATAKVARQFQSEKGLSVDGLVGRKTWDAAWEAPVA
Physico‐chemical
properties
protein length:368 AA
molecular weight:39402,1 Da
isoelectric point:10,04
hydropathy:-0,57
Representative Protein Details
Accession
6Vxjq
Protein name
6Vxjq
Sequence length
303 AA
Molecular weight
31447,15030 Da
Isoelectric point
5,39706
Sequence
MLTNLASVLHAAGLNVVEEPGWRTRGHGEMTSVQTVVCHHTAGPATGELPSLGTVRDGRPGLSGPLAQLMLGRSGTWYVIAAGLCYHAGVVLRPSYANENAIGVEAEATGVDSWPTLQYDSYARGCAALTKAYGLRVSDVLGHKEVASPLGRKSDPNFSMSDFRSRVTDYLTVEADVPIDDADVKKILTSPIDAAYTDEKGDSITVERSLGLAQRWALFGAQGAGRVEKAVAVLNAQLAKQGGEVVSVGNAVTALTAKVDALLARPAADVDEAALVAALTQAGFSAEAIAQANLAAVRAALAQ
Other Proteins in cluster: phalp2_28020
Total (incl. this protein): 39 Avg length: 274,1 Avg pI: 7,38
Protein ID Length (AA) pI
6Vxjq 303 5,39706
11ID4 247 6,58670
11JTD 220 9,97431
1Ncop 261 6,40629
1bcmV 232 8,33971
1fviI 225 6,70316
2cN8H 275 5,52074
2etaT 259 5,91736
2heWj 286 8,85475
3c0h7 334 7,80507
3ca1j 300 7,07375
4EE70 277 6,16683
4KCvG 220 6,49842
4TsDc 218 9,34058
4ggeA 340 7,93066
4nQb4 234 5,83227
4vFar 292 5,62134
5H2CJ 242 9,03449
5qRk5 305 6,62313
6H4Y4 312 5,08325
6SNam 297 9,21732
6T9Ny 232 7,18629
6T9QJ 263 8,95996
6VaVB 263 9,17793
6VbK8 264 8,96441
6W2hl 288 5,73445
6W6Bt 324 5,78078
7G97R 294 5,73525
7KWQt 256 6,59318
7iIy2 285 7,24915
7lauf 274 5,94231
7u9LF 272 6,21707
7uBx1 266 8,93882
7uPLl 325 6,18263
7wkCW 265 9,20294
7wkpF 266 8,76991
7wkqi 265 8,80330
7zhM3 242 8,26151
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_22551
1qQEX
 38 52,1% 232 2.036E-78
2 phalp2_36860
7gDKs
 246 46,7% 216 7.794E-73
3 phalp2_30398
4MRRT
 4 38,8% 219 9.181E-45
4 phalp2_12497
QM59
 22 38,8% 229 3.980E-41
5 phalp2_29173
7xGLk
 1 36,8% 201 3.129E-37
6 phalp2_16904
23UNd
 10 32,4% 228 7.901E-37
7 phalp2_10382
1L8PE
 1 37,1% 199 2.715E-36
8 phalp2_7272
3Xi6B
 1 29,2% 301 5.032E-36
9 phalp2_32798
2yYOK
 118 29,3% 259 1.212E-25
10 phalp2_2173
3X5ax
 1 29,2% 212 6.497E-21

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 6Vxjq (303 AA)
Member sequence: A0AAD1VFB9 (368 AA)
1 303 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Brevibacterium phage Rousseau
[NCBI] 		2891957 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OV696622 [NCBI]
CDS location
range 21361 -> 22467
strand +
CDS
ATGGCATACGCACTAGCGTCACGACTTGAGCGAGCGGTTAAGCGCGCAGGGGTCAAGTACAAGAAGGTTAGCGGTTGGGCGTCTCGCGGGCACGGCACCATGGGATCAATCCGTGGCGTCGTTGCACACCACACCGCCGGACCCGCATCTGGTAACGCGCCGTCACTGAACGTCGTTGCTCATGGCCGTCCTGGTCTTAGCGGGCCACTGTCCCAGCTATTCCTCGCACGCGACGGCACGGTCTATCTCGTTGCCGCTGGTGTGTCCTGGCATGCAGGCCGCGTCAACAACTCCAACTTCACCAACTCCCACGCCATCGGGATCGAAGCAGAAGCGACGGGCACTAGCTCATGGCCCGCTCATCAGGTGGATGCTTACGCGAAACTGTGTAAGGCACTGTGCGATGAGTTCAACCTGTCAACTTCCCGCGTCCGTGGGCACAAAGAGGTCTGCTCGCCTCGCGGTCGTAAGATTGACCCCAACTTCAGCATGAACGATTTCCGTGCGAAGGTTGGCGGCGCAAAGGGTGGCGTGTCCAGTGGCGGCGGTTCGACGGGTGGCGGTTCGTCTAAGTCCTACAAGACTGTCAGCTACGGCACCACGCTTGGCCTGTATGACAAGGGCGACCCCGTAAAAGAGTGGCAGATCGAAGCACTCGGATACAAGGGCAAAGAAGCTGATGGCTACTTTGGTCCCGATACAGTCAAGGACACGAAGACTGTTCAGAAGAAGTATGGTCTGAAGGCTGACGGTCTGGTTGGTCCGAAGACGTGGCCGCTGCGACTCAAGGAAGCTAAGGGCAAGTTCACTCGCCCGAAGCATGAGAAGGCTAAGGACGCACCGAAGTTCCCACTGAAGCGCGGCTGGTACTTTGGGCCTAAGTCGGGTCCAGCATCTAGCGTGTCGGGTTATTATTCGCACCGTTCGGACCTGAAGCGTTGGCAGCAGCGCATGAAGAACCGTGGCTGGACGATCGCGGCTGACGGTCTGTATGGACCGGCTACGGCTAAGGTTGCGCGTCAGTTCCAGTCGGAGAAGGGTCTTTCAGTCGATGGCCTGGTTGGTCGTAAGACTTGGGATGCCGCATGGGAAGCTCCTGTTGCGTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (6Vxjq) rather than this protein.
PDB ID
6Vxjq
Method AlphaFoldv2
Resolution 76.21
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50