Protein

Protein accession
A0AAD1VD97 [UniProt]
Representative
4KsAl
Source
UniProt (cluster: phalp2_21916)
Protein name
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
Lysin probability
100%
PhaLP type
endolysin
Probability: 81% (predicted by ML model)
Protein sequence
MKQITPYEVIDGQLKGARQVPSPNFNQRPAGTEIQMIVVHNISLPPSQFGGGYIEQFFQNKLDWSVHPYFQTIEGMQVSTHLLILRTGEVLQFVNFNDRAWHAGRSSYLAKVECNDYSIGIELEGSDDLPFEDVQYEVLTDVVTAIRQAYPEIKNHIAGHSDIAPGRKTDPGPYFKWQHFRQLLAQKKT
Physico‐chemical
properties
protein length:189 AA
molecular weight:21547,1 Da
isoelectric point:5,95
hydropathy:-0,43
Representative Protein Details
Accession
4KsAl
Protein name
4KsAl
Sequence length
183 AA
Molecular weight
19964,38490 Da
Isoelectric point
5,52466
Sequence
MPRPIRPIPSPNCDDRPPGEPVRLIVVHAISLPPDEFGGPGVEQLFTNTLDPAAHPYFASIALLRVSAHYFIRRDGEIIQFVDPDRRAWHAGVSTWRGREGCNDFSIGIELEGCDSQPFEAVQYRRLAELIRELAARYPVEAVVGHSDVAPGRKTDPGPCFEWGRLRSLLADCGGLAVGAGGG
Other Proteins in cluster: phalp2_21916
Total (incl. this protein): 20 Avg length: 191,9 Avg pI: 7,19
Protein ID Length (AA) pI
4KsAl 183 5,52466
1lqw9 189 6,70015
3S0N4 164 7,12945
3j9Pt 230 6,45955
4HbmY 221 8,87074
4UOpd 175 8,42494
4WmxG 171 9,26438
4e0TB 179 6,13846
4hsdP 180 9,53670
4hwN8 185 9,40634
5IYmg 186 7,95948
5hY0V 187 5,25303
6Lz4u 190 6,70589
6PPUL 202 6,70646
6SDko 198 7,75786
6VOYS 196 8,50539
DFpu 195 5,20431
kZQL 242 5,61645
qDH9 176 6,74937
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_35735
47Q1t
 51 41,3% 174 1.079E-43
2 phalp2_11760
45aqa
 242 43,9% 166 2.050E-40
3 phalp2_6728
24ioh
 33 42,0% 164 2.833E-37
4 phalp2_31152
3LHkO
 133 34,1% 202 8.981E-36
5 phalp2_15960
4SRAA
 2 42,7% 117 1.871E-33
6 phalp2_31626
4C5ft
 15 39,2% 168 4.773E-30
7 phalp2_21805
4f0s7
 9 32,9% 161 1.345E-27
8 phalp2_39806
zxOV
 15 33,5% 170 5.146E-25
9 phalp2_24979
ze0C
 8 28,3% 173 5.146E-25
10 phalp2_25092
1jplP
 8 32,7% 171 1.798E-24

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 4KsAl (183 AA)
Member sequence: A0AAD1VD97 (189 AA)
1 183 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Acinetobacter phage MD-2021a
[NCBI] 		2899278 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
CAKLQH020000009 [NCBI]
CDS location
range ->
strand
CDS

  




        

        
          

        
      
        

          
CDS Source ID

          

             
            [NCBI]
          


          
CDS Source

          

            CAKLQF020000013
            [NCBI]
          


          
CDS location

          

            range  -> 

            strand 
          



CDS



  

    

  




        

        
      
    
  






  

  
  

    
Gene Ontology


  





  


    
    

      

        
          

            
            Description
            Category
            Evidence (source)
          

        
        
          
          

            
              
                GO:0001897
              
            
            symbiont-mediated cytolysis of host cell
            biological process
            None (UniProt)
          

          
          

            
              
                GO:0008745
              
            
            N-acetylmuramoyl-L-alanine amidase activity
            molecular function
            None (UniProt)
          

          
          

            
              
                GO:0009253
              
            
            peptidoglycan catabolic process
            biological process
            None (UniProt)
          

          
          

            
              
                GO:0009254
              
            
            peptidoglycan turnover
            biological process
            None (UniProt)
          

          
          

            
              
                GO:0042742
              
            
            defense response to bacterium
            biological process
            None (UniProt)
          

          
          

            
              
                GO:0071555
              
            
            cell wall organization
            biological process
            None (UniProt)
          

          
        
      

    

    

  





  

  
  

    
Enzymatic activity


  





  

    
      

        

          
            

              EC Number
              Entry Name
              Reaction Catalyzed
              Classification
              Evidence
              Source
            

          
          
            
              

                
                  
                    3.5.1.28
                  
                
                
                  None
                  
                
                Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
                
                  
                  
                  
                
                
                  match to sequence model evidence used in automatic assertion
                  
ECO:ECO:0000256
                		
                

  
    ARBA:ARBA00001561
  


                
              

            
          
        

      

    
  




  

  
  

    
Tertiary structure


  





  

    
      
        
        
          

            

              

                

                  
PDB ID

                  
upi000165e95c_model

                

                

                

                  

                    Method
                    AlphaFold3 (non-commercial)
                  

                  

                    Resolution
                    -
                  

                  

                    Chain position
                    -
                  

                

                
              

            

            
            
            

              

                
Model Confidence

                

                  

                    

                    Very high
pLDDT > 90
                  

                  

                    

                    High
90 > pLDDT > 70
                  

                  

                    

                    Low
70 > pLDDT > 50
                  

                  

                    

                    Very low
pLDDT < 50
                  

                

              

            

          


          
        
        
        
      

      
        
        

        
        

          The structures below correspond to the cluster representative
          
            (4KsAl)
          
          rather than this protein.
        

        
        
          

            

              

                

                  
PDB ID

                  
4KsAl

                

                

                

                  

                    Method
                    AlphaFoldv2
                  

                  

                    Resolution
                    95.11
                  

                  

                    Chain position
                    -
                  

                

                
              

            

            
            
            

              

                
Model Confidence

                

                  

                    

                    Very high
pLDDT > 90
                  

                  

                    

                    High
90 > pLDDT > 70
                  

                  

                    

                    Low
70 > pLDDT > 50
                  

                  

                    

                    Very low
pLDDT < 50