Protein

Protein accession
A0AAD1QSB4 [UniProt]
Representative
65xN2
Source
UniProt (cluster: phalp2_13682)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSEYKYDSSHTAKSYTKTKTTKTEIVIHHWGSDGQTHDGVVRWFERAGTSAHYVISAGRVSCLVDPSFIAWHAGNWEVNKRSYGLECRPEMSAGDLETVAQVIADIWKSHKKKLPITYHKKYKATACPGRYMGKLNWLRERATEIYEGKPATKKFYVVKSGDTLSGIAKKYKTTVDKLLKLNPEIKDADKISVKQKIRIK
Physico‐chemical
properties
protein length:200 AA
molecular weight:22783,9 Da
isoelectric point:9,67
hydropathy:-0,66
Representative Protein Details
Accession
65xN2
Protein name
65xN2
Sequence length
208 AA
Molecular weight
23480,92790 Da
Isoelectric point
4,75711
Sequence
MKYIDISDWDATSFTKENRVTITNGVEILDPSVDTIVIHHWGNDGQRFEDVCNFFAGGPGTSAHFVVEAGRCAQLVEIKDIAWHAGNWAANQRSIGIECRPEMSNEDFETVAQVIADLETYYGRSFYINGHLDYYNTECPGRWYSRLEDLIRRVNDIKAGKIERGVENVPAPLPAVDVERIRAEFAELDKKLREAVEAGKQLGETLDV
Other Proteins in cluster: phalp2_13682
Total (incl. this protein): 64 Avg length: 198,3 Avg pI: 5,00
Protein ID Length (AA) pI
65xN2 208 4,75711
11spw 193 4,75358
19ZqX 202 6,58238
1HIEb 193 4,71061
1chuF 197 4,81645
1cy4z 197 4,86703
1kHlK 206 4,83077
1kINp 196 4,79541
1knuv 216 8,46059
2LBPS 202 6,58238
2aK4D 197 4,85623
3ceRy 194 4,69066
5NSKg 194 4,89449
5PL3e 202 4,64974
5TKqM 194 4,79377
5Toys 197 4,86703
5TwOK 194 4,90165
5Uht4 206 5,08024
5XcYT 206 4,96781
5XpYw 203 4,91012
67Q1b 197 4,77109
69rXC 194 4,96627
6YVZl 205 4,59347
6aogC 206 4,72425
6bqVJ 193 4,65997
6cxtn 197 4,86703
6lmMy 194 4,98469
6mU12 196 4,60751
6p4YF 197 4,74267
6uX73 233 5,70194
6wlLc 197 4,93325
70Efg 202 4,61688
7Mk8p 193 4,80280
7Mr8N 193 4,87448
7WT5k 221 4,58585
7rqyW 194 4,63740
87WPs 205 5,18891
88TSz 197 4,73153
8h9GH 194 4,85794
8hdf2 193 4,94592
8hk9H 194 4,77632
8lkG2 197 4,60120
8lpUv 197 5,00771
8oeTk 193 4,67151
8pL7c 193 4,80917
8pL9G 198 4,67889
8pLar 197 4,85623
8qVoV 194 4,80280
ES1E 202 4,66014
F28w 193 4,71385
HrSV 194 4,79121
JZ98 202 4,68094
JZug 194 4,78314
K72s 206 4,71425
KJAt 194 4,71078
N3i6 193 4,75034
Ot4e 193 5,03647
oPcN 193 4,67168
oPvY 193 4,72187
p3Ff 197 4,80582
q1uZ 193 4,89170
q1wF 193 4,86339
tpu3 193 4,80309
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_16559
7s5Uq
 12 50,3% 161 3.754E-64
2 phalp2_7901
8LrgT
 13 46,3% 136 1.415E-42
3 phalp2_25595
3X4EC
 26 43,3% 143 1.272E-41
4 phalp2_25234
40uRC
 5 31,6% 193 1.924E-39
5 phalp2_7211
3lM9H
 3 42,8% 168 6.745E-39
6 phalp2_26039
6YX1D
 5 43,4% 138 8.281E-38
7 phalp2_12749
88ep5
 5 37,4% 139 1.862E-33
8 phalp2_13806
716PV
 1 27,3% 161 1.967E-23
9 phalp2_4921
6qvcZ
 45 28,2% 198 7.525E-17
10 phalp2_24069
8amt4
 8 32,1% 137 3.501E-16

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 65xN2 (208 AA)
Member sequence: A0AAD1QSB4 (200 AA)
1 208 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Glutamicibacter phage Voltaire
[NCBI] 		2891955 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OV696617 [NCBI]
CDS location
range 8134 -> 8736
strand +
CDS
ATGAGTGAATACAAATACGATAGCAGCCACACCGCCAAATCCTACACCAAAACCAAGACCACCAAAACGGAAATCGTCATCCACCACTGGGGTTCCGATGGTCAGACTCATGATGGTGTGGTTCGATGGTTCGAACGCGCTGGCACCAGTGCGCACTACGTTATTTCCGCTGGCCGCGTGTCCTGCCTGGTTGATCCTAGCTTCATTGCGTGGCACGCCGGGAACTGGGAAGTGAACAAGCGTTCCTATGGCCTGGAATGCCGACCTGAAATGAGTGCTGGCGACCTGGAAACCGTGGCACAAGTGATCGCTGACATTTGGAAATCGCACAAGAAGAAGCTGCCGATCACTTACCACAAGAAGTACAAGGCGACCGCGTGCCCTGGCCGGTACATGGGTAAGCTGAACTGGCTGCGTGAGCGTGCCACCGAAATTTACGAGGGTAAGCCAGCGACCAAGAAATTCTATGTGGTCAAGAGCGGCGACACGCTATCAGGCATTGCGAAGAAGTATAAGACTACGGTGGATAAGTTGTTGAAGCTGAATCCGGAAATCAAGGATGCTGATAAGATCAGTGTGAAACAGAAAATTCGTATCAAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (65xN2) rather than this protein.
PDB ID
65xN2
Method AlphaFoldv2
Resolution 91.23
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50