Protein

Protein accession
A0AA96UXU9 [UniProt]
Representative
8LlFs
Source
UniProt (cluster: phalp2_27885)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MADPTWLPDELKRWGLKVEYIGDPMNDGHGDFRQCLGVIGHHTAGGGSNDWRIVKYGRPDLPGPLAQLVLEKDGSVKFIAIGVCWHAGRGRWKSKRPNPGIRDNDANWYTIGVEAVSRGTPDAQGRFDWTDAQIRSYKLIVAAICSKIGKHVDEGFLGHKEYSSEGKIDPAGIDLDAFRRDVQALIDNPPWKAGTPVANENKEIGEIHFQIAQPHKVREAFQKVFYNIKDGNFWLRAGLADIWNEVVWDGYVNPVDLLDGKPDPDKAPKDTPLEKLARRGSLVSYVLATYREATLARREAEAARKDIVALTEKVNAIAAKV
Physico‐chemical
properties
protein length:321 AA
molecular weight:35726,0 Da
isoelectric point:7,10
hydropathy:-0,48
Representative Protein Details
Accession
8LlFs
Protein name
8LlFs
Sequence length
463 AA
Molecular weight
51702,06720 Da
Isoelectric point
7,13872
Sequence
MIRIADYTRYPEAFKKFGINFTEEPGWRERGHGDIVKVQFIVIHHTAGGNDAGDIRIVTYGRSDLPGPLSQLVLKRNGDPHIVAQGVAWHAGYGPAMWGAPAGNGNYYSIGIEGVSNGYNDWTEAQRANYPKVVAALLKDMGLPSDRWIFHRDYNKRDGKIDPAGFDAGWFGREVNRWYNDGGQPVESAIQAKRRENAWLGNKTIPEDERKTLDGVGRYASYDNGYVYWHPSIGARTIKNGPIWDYYAAQKWEQGFLGYPINDTHDLGDGRSAQAFQNGSVYSGPKGTYLVMGQIGAEWARLNWEKGPLGMPISEELKSPAGIIQKYENGTIFYTVAGGARATYGLIEQTRSRLDQEFSFGYPENSESPCFNNTGRFNHFVKASIYWKRGTETAFAVADPAREIWGKMGWEQGRLGYPISTTSPKFGSLEIRRTDFEGGSIEYNTKTGEITLIISGKVVDLPK
Other Proteins in cluster: phalp2_27885
Total (incl. this protein): 25 Avg length: 352,0 Avg pI: 6,46
Protein ID Length (AA) pI
8LlFs 463 7,13872
14i3O 384 8,20123
1hMCQ 465 6,19849
6OdML 257 4,77330
7cMxA 255 4,63195
7dhMs 285 10,75773
7dhR2 366 7,28974
7jGk8 366 6,76346
7jGxG 366 6,59755
7jNtw 357 7,25495
7lGqW 258 4,66764
7lGuK 258 4,65764
7qJuc 270 6,30665
7tGqP 366 7,30213
7ukgS 255 5,60918
7zGK5 255 4,72670
7zST3 381 6,11942
8HMRE 366 6,95672
8HMRP 366 6,56680
8HMRS 366 6,97906
8LhQw 466 5,44315
8N1kz 381 6,01427
A0A2D1A400 463 7,13872
A0A7D5KAL8 465 6,19854
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_6589
1gMiR
 2 48,6% 473 5.890E-142
2 phalp2_9416
8Lrse
 65 34,7% 351 4.050E-127
3 phalp2_7159
2QxCN
 1 25,0% 512 5.429E-52

Domains

Domains [InterPro]
Ami2
LGFP
Representative sequence (used for alignment): 8LlFs (463 AA)
Member sequence: A0AA96UXU9 (321 AA)
1 463 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08310

Taxonomy

  Name Taxonomy ID Lineage
Phage Rhodococcus phage MacGully
[NCBI] 		3069508 Zierdtviridae >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OR283216 [NCBI]
CDS location
range 38437 -> 39402
strand +
CDS
ATGGCTGACCCGACTTGGCTCCCTGACGAGCTCAAGCGCTGGGGTCTCAAGGTCGAGTACATCGGTGACCCGATGAACGACGGCCACGGGGACTTCCGGCAGTGCCTCGGGGTGATCGGTCACCACACGGCGGGTGGCGGGTCGAACGACTGGCGCATCGTGAAGTACGGCCGGCCGGACCTCCCCGGCCCGCTGGCGCAGCTCGTCCTGGAGAAGGACGGGTCGGTCAAGTTCATCGCGATCGGCGTCTGCTGGCACGCGGGACGCGGCCGGTGGAAGTCCAAGCGGCCGAACCCCGGCATCCGCGACAACGACGCGAACTGGTACACGATCGGTGTCGAGGCCGTCTCGCGCGGTACCCCGGACGCCCAGGGCCGGTTCGACTGGACCGACGCCCAGATCCGGAGCTACAAGCTCATCGTCGCGGCGATCTGCTCGAAGATCGGCAAGCACGTGGACGAGGGCTTCCTCGGCCACAAGGAGTACAGCTCCGAAGGCAAGATCGACCCCGCTGGTATCGACCTCGATGCGTTCCGGCGGGATGTCCAGGCCCTCATCGACAACCCTCCGTGGAAGGCAGGCACTCCAGTGGCCAACGAGAACAAGGAAATCGGCGAGATCCACTTCCAGATCGCGCAGCCGCACAAGGTGCGGGAGGCCTTTCAGAAGGTCTTCTACAACATCAAGGACGGCAACTTCTGGCTCCGCGCGGGCCTGGCCGACATCTGGAACGAGGTCGTCTGGGACGGCTACGTGAACCCGGTGGATCTGCTCGACGGGAAGCCGGACCCGGACAAGGCTCCGAAGGACACCCCGCTGGAGAAGCTGGCTCGTCGGGGCTCGCTCGTCTCCTACGTGCTCGCGACGTACCGGGAGGCCACCCTGGCGCGCCGCGAGGCCGAGGCCGCGCGCAAGGACATCGTCGCGTTGACGGAGAAGGTCAACGCGATTGCCGCTAAGGTGTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8LlFs) rather than this protein.
PDB ID
8LlFs
Method AlphaFoldv2
Resolution 93.08
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50