Protein

Protein accession
A0AA50Q8W4 [UniProt]
Representative
7wFIi
Source
UniProt (cluster: phalp2_38224)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MDFSNLIADKQYFINRSFTRGREGKDIEFIVLHHNAGVRQSTETVGNFWEGAGTSAHYQVEDDGTIGQTVHDYDTAYHAGSWEANTKSIGIEHANITGPNDGTPWDISEATIKYGGYLTGALCYGYGLGSPTWGWNVFPHSDFTSTACPFQLRDKYRDAYMGYAVEMYNRLSGGEVTPAYAPPAVTTVDRREQLAKALHCSADASDLNLRALVLVSASDWGGNNFPCGIEFAQEVVGTEVDGVWGELSEEAHDETVAEVQRILGVTTDGIVGPITATELTRVINN
Physico‐chemical
properties
protein length:285 AA
molecular weight:31087,8 Da
isoelectric point:4,61
hydropathy:-0,33
Representative Protein Details
Accession
7wFIi
Protein name
7wFIi
Sequence length
289 AA
Molecular weight
32445,38390 Da
Isoelectric point
5,43105
Sequence
MDYKNLKADVELYIPKHFTSGRRGRRIEFVGIHHTAAVGLTVRGCYNVWQTRPASAHYLVQRDGVIGQLVHDRDTAWALGDFDANCRSVNIEHANNGSDPWTVSEACLDSGAHLVAALCIAYGLGRPMWGGNVRPHSMYMATACPGELALSQRDAYIARAQRYYDMMAAGAADEEEEVKDEDIEKIAQRVWTIEKGRETHDRCYRMTSMLKAMCGIGAEDTTDPKSIAGEIYNRTIDRWARCTAMLKAICAIPKESMAIEDVPEELDFRLSDWDVERIAERVAELVADR
Other Proteins in cluster: phalp2_38224
Total (incl. this protein): 29 Avg length: 304,8 Avg pI: 7,54
Protein ID Length (AA) pI
7wFIi 289 5,43105
1mswt 306 9,60967
4f33m 339 4,89647
4f5fV 326 5,30344
4f87y 342 5,20534
4j7Wf 306 9,31183
5tXeq 298 6,99025
6YYfJ 283 9,55384
6f9ah 279 9,28359
6kZOo 283 9,39590
76gi3 306 9,36624
76jVJ 304 9,34638
79L2k 302 9,32111
7BMJV 285 9,04815
7BY9T 283 9,13093
7BfGQ 311 9,55597
7bbTK 315 5,44264
7bjh5 306 9,38668
7gMAX 303 5,29895
7gSfv 304 9,25407
7rhFE 323 4,74977
7rhG0 323 4,65957
7uG4v 303 9,36624
7vOUc 306 9,00071
7wXYL 328 9,45669
8icmx 289 5,82710
8tPlD 266 6,02984
A0A8S5UDH9 346 4,78973
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_17249
3TCUc
 1 32,0% 181 1.195E-31
2 phalp2_1605
81mMl
 270 27,9% 197 2.985E-17
3 phalp2_14594
5ioYm
 94 27,2% 191 1.291E-12
4 phalp2_15294
1dl82
 2 25,9% 204 4.530E-09
5 phalp2_12419
gjha
 11 22,5% 195 1.429E-06

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 7wFIi (289 AA)
Member sequence: A0AA50Q8W4 (285 AA)
1 289 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Caudovirus D_HF4_2
[NCBI] 		3071195 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OR148985 [NCBI]
CDS location
range 6514 -> 7371
strand +
CDS
GTGGATTTTTCCAATCTCATTGCTGATAAGCAGTACTTCATCAACCGATCGTTTACCCGAGGTCGCGAGGGTAAGGACATCGAGTTCATCGTTCTGCACCACAACGCCGGAGTCCGTCAGTCTACAGAGACTGTAGGTAACTTCTGGGAAGGCGCCGGAACTTCGGCGCACTACCAGGTCGAGGACGATGGCACCATCGGACAGACTGTCCATGATTACGATACTGCGTACCATGCCGGTAGCTGGGAAGCTAACACCAAGTCCATCGGTATCGAGCACGCCAACATCACGGGTCCCAACGACGGAACACCTTGGGATATCTCCGAAGCCACGATCAAGTACGGTGGTTACCTTACCGGTGCTCTCTGCTACGGATACGGTCTTGGTTCGCCCACGTGGGGATGGAACGTGTTCCCGCATTCGGACTTCACTTCGACAGCTTGCCCGTTCCAGCTCCGAGACAAGTACCGCGACGCCTACATGGGCTACGCAGTTGAGATGTACAACCGTCTGTCCGGCGGAGAAGTCACCCCTGCGTACGCACCTCCCGCGGTCACTACGGTTGATCGCCGTGAGCAGCTTGCCAAGGCTCTGCACTGCTCCGCAGATGCTTCTGACCTGAACCTTCGGGCGCTCGTCCTTGTCTCTGCATCTGACTGGGGCGGCAATAACTTCCCGTGCGGAATTGAATTCGCTCAGGAAGTCGTCGGTACCGAGGTCGATGGTGTCTGGGGTGAGCTTTCGGAAGAGGCTCACGACGAAACCGTCGCTGAGGTTCAGCGAATCCTCGGCGTGACGACCGACGGTATCGTAGGCCCGATCACTGCGACTGAACTCACTCGCGTGATCAATAACTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7wFIi) rather than this protein.
PDB ID
7wFIi
Method AlphaFoldv2
Resolution 73.97
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50