Protein

Protein accession
A0AA50ACT4 [UniProt]
Representative
6jLqY
Source
UniProt (cluster: phalp2_12158)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MRFLFRPIKRNYRSRGKVGIRYLVIHDTGNLNKGAGALNHRNYVENNERGASAHYFVDDKVIVQYVGDSLAAGSVGDGKGRFGITNSNSLSIEMCINSDGNYLKMYKNTVELTKNLMKKFNIPWERVVRHYDASRKNCPGHMSKNNWEKWKKFKEDIKKPIEWKIDLSKDSEFGVNKVMEKDYKGHWAEKAIDEVKEAGIMKGYEDGSFKPDEKITRAEVAVIVSKLLKKN
Physico‐chemical
properties
protein length:231 AA
molecular weight:26570,1 Da
isoelectric point:9,58
hydropathy:-0,76
Representative Protein Details
Accession
6jLqY
Protein name
6jLqY
Sequence length
386 AA
Molecular weight
43388,05730 Da
Isoelectric point
9,66969
Sequence
MKFLFRPIKRNYRSRGKVGIRYIVIHDTGNPNKGAGALNHRNYVENNKRGASAHYFVDDKVIVQYVGDSLSAGSVGDGRGRYGITNANSLSIEMCINSDADYVKTYKNTVELTKNLMRKFNIPIDRVVRHYDASRKNCPGHMRQNNWSKWLKFKDDIKKPIEWQIDLSKDSNFGSEVKVEEKDYFTTNGLKIIKTTADKIYIQQLGGKTLRQVGAYGINGTFFDTAKPGLKESTWGIAVNNGKPIGPNADKNHWKKNIKRATMIYGDGKVTVEYANNLKDIKRDKIDWAIGGVGLYPSYNPNYEQVPKDILRCTEHTGIAFKGHVIFLVVTDKPCTMGNFRAKICNLDIDGAIALDGGGSTQMFYKNNFGVHTTRKLNNIIGVKGV
Other Proteins in cluster: phalp2_12158
Total (incl. this protein): 12 Avg length: 382,8 Avg pI: 9,03
Protein ID Length (AA) pI
6jLqY 386 9,66969
5O6Kn 446 7,98533
60iIp 399 9,55359
67awF 408 8,88576
6cZ0d 383 9,61219
6gJW8 351 6,39697
6omTK 386 9,60916
7pnWR 426 8,54175
7vCJW 391 9,37733
7z6bF 395 9,72030
A0AA49X4T4 391 9,38094
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_3173
8mQDL
 1 27,7% 361 6.642E-31
2 phalp2_13250
366VA
 3 26,3% 383 1.802E-29
3 phalp2_28578
8ccIC
 2 27,3% 399 1.596E-27
4 phalp2_31516
3ZAQL
 7 24,5% 415 4.350E-20

Domains

Domains [InterPro]
Ami2
NAGPA
Representative sequence (used for alignment): 6jLqY (386 AA)
Member sequence: A0AA50ACT4 (231 AA)
1 386 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF09992

Taxonomy

  Name Taxonomy ID Lineage
Phage Firmicutes phage HS16
[NCBI] 		3056394 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OQ890324 [NCBI]
CDS location
range 18003 -> 18698
strand +
CDS
ATGAGATTTTTATTTAGACCGATAAAAAGAAATTATCGATCACGTGGAAAAGTGGGAATAAGATATCTAGTTATCCATGATACAGGAAATCTCAACAAAGGAGCAGGAGCTTTAAATCACAGAAATTATGTGGAAAATAACGAAAGAGGAGCAAGTGCCCACTATTTTGTTGATGATAAAGTTATAGTTCAGTATGTAGGAGATTCTTTAGCAGCGGGTTCAGTGGGAGATGGTAAGGGAAGATTTGGAATTACAAATAGCAATTCACTATCTATTGAAATGTGCATAAATTCAGATGGAAATTATTTAAAGATGTACAAAAACACAGTTGAATTAACAAAAAATCTTATGAAAAAATTTAACATACCATGGGAAAGAGTAGTAAGACATTACGATGCAAGCAGGAAAAACTGTCCTGGACACATGAGTAAAAACAACTGGGAAAAATGGAAAAAATTTAAAGAGGACATCAAAAAGCCGATTGAGTGGAAGATAGATTTATCAAAAGATTCAGAATTTGGAGTGAATAAAGTTATGGAAAAAGATTATAAAGGTCATTGGGCAGAAAAGGCAATTGATGAAGTAAAAGAAGCTGGAATCATGAAAGGTTATGAAGATGGAAGTTTTAAACCTGATGAAAAAATTACCAGGGCGGAAGTTGCCGTTATTGTTTCAAAATTACTAAAAAAAAATTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (6jLqY) rather than this protein.
PDB ID
6jLqY
Method AlphaFoldv2
Resolution 93.95
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50