Protein

Protein accession
A0AA49X3Y8 [UniProt]
Representative
5Zqsx
Source
UniProt (cluster: phalp2_30585)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MDWMNVEPDVYRLLNKHYTPGRGGQRIKYIVRHHNAGILTIDGCWDVWQTRQASAHYQVENSGRIGQLVNDSDTAWHAADLLRNQQSIGIEHANSGGANQDWPISDTVIKQGARLAAALCRYYNLGRPEYGKNIKDHKETGQTSCPYHLAAGGKYHQAWMNEAQRFYDELTHGGGQKPAAAKPVKKASKPVLTPKYFTDFLTGYLGPQFDAIQEIWRQLRGPSGNGWEQLGQNSKGQNLTLVDAVAALRSDVARIEKKLDEKGKK
Physico‐chemical
properties
protein length:265 AA
molecular weight:29681,0 Da
isoelectric point:9,19
hydropathy:-0,75
Representative Protein Details
Accession
5Zqsx
Protein name
5Zqsx
Sequence length
269 AA
Molecular weight
29808,47960 Da
Isoelectric point
5,77100
Sequence
MDFNKLQADVNMLLTRHFTKGRSGHKLKFTVVHYNAGDLTIEQCYNIWQSYEASAHYQVESGGRIGQLVNDSDTAWHARDWDANCESIGIEHANQGDSITDACLESGAHLLAAIHKFYGLGRPEWGVNVFPHSHFAATQCPGPLREGTTYHNRYMARAQQWYDAMEAGTEPGDAPTGETTQPSGEHSGQGFGGRYRCTVDYLRVRDAPGLSGTEVAHYSAGETVTLDDWYKIADGYVWGRYTGYSGKVRYVAVGKATGKPEADDYLVRV
Other Proteins in cluster: phalp2_30585
Total (incl. this protein): 178 Avg length: 320,8 Avg pI: 7,16
Protein ID Length (AA) pI
5Zqsx 269 5,77100
13Dpe 347 5,37551
1FRPV 357 5,08518
1HtjX 356 5,10939
1lWIH 267 6,17564
1ohGI 267 6,14756
2lVSh 273 6,24293
2mbUq 362 4,71669
2ttt 352 8,05412
3GNsn 279 6,18314
3KMG3 279 6,12056
3e0Le 258 8,88060
3jRRc 267 6,17569
3jTPX 263 6,23378
3m157 354 8,85017
3mtO1 364 7,50476
3muZU 354 5,09797
3p73E 267 6,34189
3pHwY 354 9,09618
3pQGy 364 7,04357
3pT8z 367 7,04357
3rnpg 244 7,21482
3rpii 353 5,70711
3sH06 345 9,14544
3sMT0 347 7,90629
3sp3n 267 6,43704
3syHB 265 6,17882
3tQfR 365 7,50948
3tkOX 381 9,23298
3to6Z 345 9,28862
3vSMx 267 6,02609
3vXaT 267 6,23606
3wAtE 353 6,38060
3wsUV 338 9,31196
3xABO 355 9,05950
3xBGz 353 6,38225
3xJ27 338 9,21706
3yH8t 354 8,94481
3yPjq 267 6,28522
4VPAe 267 6,61955
4z8Kx 267 5,88434
5HMZ7 312 4,92819
5JTZh 381 9,42265
5KtfZ 353 8,05798
5L9Mq 336 8,86261
5LbSU 267 5,95618
5MAM3 261 6,23378
5Mmaz 361 7,50476
5OKG1 267 6,23236
5OX6B 267 6,02609
5P3xS 354 8,77732
5PIR4 353 5,88473
5PiRh 364 7,03862
5Pxti 364 7,03487
5QwvN 364 7,91241
5Rqiw 365 7,50948
5Ru5x 352 8,92199
5Sc6z 354 8,85333
5SzlT 355 8,87499
5TJKa 354 9,02649
5TzgH 355 8,67914
5UMI9 344 9,22744
5UPmL 326 5,98693
5UeHd 335 8,85733
5VO42 267 6,34189
5VQn1 365 7,92041
5VeDR 274 6,70293
5W7BV 286 5,03795
5WQLl 361 7,50050
5Wo0E 336 8,98246
5X39o 267 6,14892
5YQip 267 6,17564
5Zppd 269 5,17283
5sS2 267 6,39197
5zml 266 6,07367
604rs 365 7,49174
63CvB 274 6,23657
64dj5 361 7,04357
65Z8D 274 6,30080
65ivh 354 8,79479
65l32 301 8,57212
66NwM 345 4,89022
66sIq 326 5,78117
67VIg 354 9,11133
693Wg 267 6,39197
69One 267 6,39157
6aKIP 311 5,85972
6aQm8 364 6,69571
6b4gI 345 9,38133
6bW24 362 4,64172
6chsw 326 5,98693
6cktT 267 6,53509
6dE2r 355 8,86900
6dIqN 320 8,36176
6dP06 266 6,02456
6dWvt 365 7,50948
6dvhX 320 8,75830
6eRXd 267 6,18052
6eUck 279 6,29380
6epN0 263 6,08714
6eqm1 364 7,50362
6fIPK 354 8,77732
6fsoC 331 6,07179
6hFkU 347 7,90874
6hxcR 354 8,88731
6iAG9 333 8,90013
6iCnc 355 8,28259
6iIvA 355 8,95764
6iPbV 269 5,67460
6iQ6f 365 8,13219
6iRht 265 6,23509
6iaET 358 6,64717
6ihhm 267 5,88121
6ikN8 285 7,92408
6iuQQ 326 5,98693
6iz2k 274 6,44375
6jDbE 364 6,44130
6jt2p 354 8,95119
6kbZw 354 8,95770
6kgbC 354 9,03339
6kqL5 336 5,74190
6kuHI 326 5,78117
6kybu 366 7,85136
6l2n3 336 8,89749
6lr1Z 354 9,21410
6maUh 358 6,38787
6mgEJ 358 7,46105
6nCno 266 6,17564
6nYJg 382 9,14363
6nkfw 362 7,49464
6pOPD 266 5,96061
6qEPo 383 9,33085
6s3xB 358 6,64717
6sdV7 355 8,87499
6sjnk 312 5,86086
6tqGm 335 8,90342
6tyzi 283 6,23856
6u13a 356 9,00303
6u62U 354 8,94481
6uPYA 265 6,09049
6vMIk 285 7,48748
6wb6Q 264 6,09026
78hWy 329 6,00052
7UOd9 364 7,04357
7ViZE 354 8,84450
7WOSt 359 5,96653
7Y7NB 358 7,49027
7Y8gP 364 7,52022
7bNcK 267 6,02035
7c3i1 267 6,39276
7o1Rt 267 6,08862
7rS5a 354 8,87499
7s5Vj 268 5,27519
7tZ2f 300 9,53231
7ubin 265 5,61964
7vbur 267 6,09112
81MES 317 7,66965
82Td3 354 9,08883
82txH 267 6,23486
84rT6 294 6,23026
887cP 340 4,67145
887vG 348 4,57431
88cVk 366 6,59471
8K39K 290 6,12090
8dnoB 274 7,91835
8id9k 381 9,51800
8ie7D 364 7,03908
8loNk 359 8,13225
8neB6 267 6,08862
8pGEo 301 4,42193
8q4KT 267 6,17677
8rjpy 350 4,65650
8tOYb 267 6,74562
8vSmd 354 9,08883
8vtoj 365 7,89855
ogpU 349 8,96667
ynDF 267 5,95811
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_6225
6oM64
 106 54,0% 187 6.216E-84
2 phalp2_4921
6qvcZ
 45 60,6% 188 1.453E-79
3 phalp2_29674
8k36v
 96 30,1% 196 8.653E-49
4 phalp2_39194
3fUf1
 5 28,1% 174 6.094E-46
5 phalp2_5901
5Lu6c
 99 31,6% 265 1.224E-43
6 phalp2_12977
3h4lU
 700 25,5% 223 7.941E-43
7 phalp2_29922
8b3eG
 10 31,3% 239 2.421E-36
8 phalp2_37526
3iAYu
 8 25,0% 196 8.762E-34
9 phalp2_26095
7w4x3
 24 23,5% 242 3.024E-33
10 phalp2_29855
40doy
 8 25,3% 245 7.654E-33

Domains

Domains [InterPro]
Ami2
Unannotated
Representative sequence (used for alignment): 5Zqsx (269 AA)
Member sequence: A0AA49X3Y8 (265 AA)
1 269 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Corynebacterium phage HS03
[NCBI] 		3056390 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OQ890313 [NCBI]
CDS location
range 22072 -> 22869
strand +
CDS
ATGGATTGGATGAACGTGGAGCCGGACGTATATCGGCTCCTGAATAAGCACTACACACCAGGCAGGGGTGGGCAGCGCATTAAGTACATTGTGCGGCACCACAACGCCGGTATTTTGACCATCGACGGGTGCTGGGATGTTTGGCAGACGCGGCAGGCTAGTGCCCACTATCAGGTGGAGAACTCGGGCCGCATCGGCCAGCTCGTCAACGATTCCGACACGGCTTGGCACGCTGCCGACCTGCTCCGTAATCAGCAGTCAATCGGCATTGAGCACGCCAACAGCGGCGGAGCGAACCAAGACTGGCCCATCTCGGACACCGTAATCAAACAGGGCGCACGCCTCGCTGCGGCGCTGTGCCGCTACTACAACCTCGGACGGCCTGAATACGGCAAGAACATCAAAGACCATAAGGAAACCGGCCAAACCTCATGCCCATACCACCTCGCGGCAGGCGGCAAGTACCACCAGGCATGGATGAATGAGGCGCAGCGTTTCTATGACGAACTCACCCACGGCGGAGGGCAAAAGCCTGCCGCTGCTAAGCCGGTGAAGAAAGCGAGCAAGCCAGTGTTAACACCGAAGTATTTTACTGACTTTCTGACGGGGTATTTAGGCCCCCAGTTCGACGCTATCCAGGAAATATGGCGGCAACTACGTGGCCCCTCGGGTAACGGCTGGGAGCAATTAGGGCAGAACAGCAAGGGCCAGAACCTCACCCTTGTCGATGCGGTGGCCGCGCTCCGATCTGACGTGGCCCGCATTGAGAAGAAGCTAGACGAAAAGGGGAAGAAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (5Zqsx) rather than this protein.
PDB ID
5Zqsx
Method AlphaFoldv2
Resolution 93.27
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50