Protein

Protein accession
A0A9Y1YXN7 [UniProt]
Representative
81gAb
Source
UniProt (cluster: phalp2_14020)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSVFTNSYIPINKYTRPGLKLQGVKKCVLHYTANPGAGADNHRRYFSNAQVYASAHIFVDKTEAICIIPLNEVAYHANDIQQRDSAGNPYRGVAALKPNANFLSIGVEMCLEKDGSFHSDTVERTEDVFVELCNKFGLDPIDDIVRHYDITHKNCPAPWVSNGQKFVDFKNRVKAKMSGKSVSKASPTKPTTSSPSSSSAVSGSLKSKVDGLRFYSKPSWEDKDVVGTVNKGIGFPTVVEKVKVGSAYQYKVKNSKGATYYITASDKYVDVTGSVKTSSSAPKTTSTSSSSSSIKSVGKIKITGVSSAAIVMDKPDRNSSKNIGTVKLGNTVSISGSVKGKNNSKGYWEVIYNGKRGYISGQFGSKI
Physico‐chemical
properties
protein length:367 AA
molecular weight:39654,4 Da
isoelectric point:9,63
hydropathy:-0,43
Representative Protein Details
Accession
81gAb
Protein name
81gAb
Sequence length
293 AA
Molecular weight
32618,73860 Da
Isoelectric point
8,64213
Sequence
MATWKNQFVRLNEYSRPGLKLKGVRKLIVHWTANPGASAANHFKYFDSTIVAAKRYASAHIFVDKTEALCIVPLDEVAYAANDGTYRGIPALSPNANFYSISVELCVEKDGTFHAETLARAAQVFSELCKTYDLDPMSDIVRHYDVTHKNCPAPWVSNARPFEEFKARVKSASLPKVEVNTLAKSELVGRKLTIKTNGLWYYNTPRWDVLGGEGLAGDTFIITEELTVNGARMVKCHDGKYRTADPAHVDITPKPSDWPYRVVIDGITHAQAAEIVNEIQAKYKNAKAEGQAK
Other Proteins in cluster: phalp2_14020
Total (incl. this protein): 48 Avg length: 314,6 Avg pI: 9,41
Protein ID Length (AA) pI
81gAb 293 8,64213
1GA78 249 9,41034
1GVUl 359 9,75537
1HvhT 355 9,74635
1cmvU 260 9,52773
1jLjd 265 9,61206
23uTj 277 7,58934
2X0iv 331 8,67997
3VDzp 248 7,60769
3WCyO 249 8,05334
3n0PR 363 9,77491
4hchr 310 9,67311
5i565 258 9,76420
5nMH2 259 8,22483
750iZ 309 9,79792
75OVP 362 9,79805
75alp 262 9,67363
78uSd 362 9,74409
7bePb 267 9,77484
7cHUU 265 9,81256
7d8ci 362 9,78645
7dJXg 236 9,60967
7enls 274 8,16674
7i7Z9 346 9,80340
7kjBL 364 9,70257
7oI2p 265 9,85001
7oaqc 262 9,70915
7oo0z 363 9,72288
7pZqF 311 9,33362
7qPux 362 9,81662
7qQ53 363 9,75292
7u5Pi 362 9,78625
7uzSV 262 9,57028
7xMDJ 355 9,76672
7yjM9 263 8,80917
cJcz 342 8,67159
A0A7H0X9I6 367 9,60065
S6BFI4 222 9,63817
A0A0U4J8N7 242 9,20430
A0A7G9URF8 367 9,62534
A0A7G5CI43 367 9,66531
A0A7G5CIL8 367 9,66531
A0A9Y1YXH3 367 9,62534
A0A9Y2DZY1 367 9,66531
A0A9Y2E0P8 367 9,66531
A0AAE9BQP8 367 9,65899
D6QWM6 338 9,36386
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_37528
3iFdV
 13 51,1% 211 1.227E-77
2 phalp2_15149
8JpFB
 23 34,2% 350 3.416E-69
3 phalp2_11225
6wIAx
 104 29,5% 261 5.021E-53
4 phalp2_19907
6axIZ
 11 27,0% 255 1.579E-46
5 phalp2_3987
7ylun
 65 27,1% 214 8.213E-35
6 phalp2_3411
3PRty
 2 22,6% 247 6.221E-31
7 phalp2_22264
7pxLN
 64 25,0% 220 9.551E-26
8 phalp2_29527
23SbO
 38 26,0% 238 5.943E-25
9 phalp2_36615
23Gpv
 52 25,5% 247 1.684E-23
10 phalp2_20199
83ZcC
 8 22,5% 293 3.921E-21

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 81gAb (293 AA)
Member sequence: A0A9Y1YXN7 (367 AA)
1 293 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage SPbetaL6
[NCBI] 		3053440 Spbetavirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OQ921346 [NCBI]
CDS location
range 36254 -> 37357
strand -
CDS
ATGTCAGTTTTCACTAATAGCTACATTCCAATCAATAAGTATACTAGACCAGGTTTGAAATTACAGGGGGTGAAAAAATGCGTCCTCCACTATACAGCCAATCCGGGCGCCGGCGCAGACAATCATCGCAGATACTTCAGCAATGCACAAGTTTATGCATCAGCTCACATTTTCGTAGATAAGACTGAAGCAATTTGTATCATTCCGTTAAATGAAGTAGCTTACCATGCTAATGATATTCAGCAAAGAGATAGTGCCGGGAATCCTTATCGAGGAGTTGCTGCGCTGAAACCTAACGCTAACTTTTTATCTATTGGAGTTGAAATGTGCCTTGAGAAAGACGGTTCGTTCCATTCAGATACAGTTGAAAGAACTGAGGATGTATTCGTTGAATTATGTAATAAGTTTGGTTTAGATCCTATTGATGATATTGTTCGTCATTATGACATCACCCATAAGAATTGCCCTGCACCATGGGTATCTAACGGCCAAAAATTTGTAGACTTTAAAAATCGAGTAAAGGCAAAAATGTCAGGCAAATCTGTTTCAAAAGCTTCTCCAACTAAACCAACAACCTCCTCCCCTTCCTCTTCATCAGCAGTAAGTGGTTCACTAAAATCAAAAGTTGACGGTCTTCGCTTCTATTCAAAACCATCTTGGGAAGATAAAGATGTTGTCGGCACAGTAAATAAAGGCATTGGATTCCCTACAGTTGTAGAGAAAGTTAAAGTTGGATCTGCCTATCAATACAAAGTTAAGAACTCAAAAGGCGCTACATATTACATCACTGCTTCTGACAAATATGTTGATGTTACAGGATCAGTAAAAACCTCTTCCTCTGCCCCAAAAACAACATCAACTTCTTCAAGCTCCTCATCTATTAAATCAGTAGGAAAAATCAAAATTACTGGTGTTTCAAGCGCAGCGATCGTAATGGACAAACCTGATCGAAATAGTTCAAAAAATATTGGCACAGTCAAGCTTGGAAACACTGTTTCAATTTCTGGTTCAGTTAAAGGTAAAAACAATTCAAAAGGTTACTGGGAAGTTATTTACAACGGTAAACGTGGATACATCTCAGGACAATTCGGTTCAAAAATTTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (81gAb) rather than this protein.
PDB ID
81gAb
Method AlphaFoldv2
Resolution 92.51
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50