Protein

Protein accession
A0A9Y1YVQ3 [UniProt]
Representative
7cEL4
Source
UniProt (cluster: phalp2_29138)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MAIKARQMLVSPEKYSIKCPYALSASFITFHNTYNDAPAQNEVSYMIGNNNEVSFHFAVDDKEVVQGIPTNRNAWHTGDGSGVNSGNRTSIGVEVCYSKSGGERYRKAEALAIKFIAQLLKERGWGVDRVKKHQDWSGKYCPHRVLDEGRWNAVKSAIAAELKALGGNTSSSSSKPTKVVKTNGSYVKNTVIADSLNVRTQRNANSSIVLTLPKGSTVQYQKGSTQNGWGYIKYTNSKGATYSGYVNVKYIKSDGELGQSTPKAKPTSKPNSSGIKSVGEIKIVGVKSAAIVMDRPDKNKAKNLGTVTLGDTLSISGSVKGKNNSNGYWEVIYKGKRGYISGQFGSTI
Physico‐chemical
properties
protein length:348 AA
molecular weight:37688,1 Da
isoelectric point:9,79
hydropathy:-0,54
Representative Protein Details
Accession
7cEL4
Protein name
7cEL4
Sequence length
378 AA
Molecular weight
42303,62920 Da
Isoelectric point
9,31686
Sequence
MTIPVRQKLVPSSKYKIKCPHVMNPSYITFHNTANDASAENEVTYMNSNNNSVSYHFAVDDKEVVQAVSLNRNAWHCGDGSGAKSGNRTSIGVEICYSKSGGERYRKAEELGIKFIAQLLHERGWGISRVKRHQQWSGKYCPHLVLQEGRWNEVLQRIQNELDALQGKVVETPVTQSGPSSSTAPTLLKRGDAGTYVKGLQGKLQSLGFNIGKYGIDGQYGPSTESAVKAFQKKYGLAADGIAGTLTLNKLEQVYKVAQQPKEKPVTPPKKEEPKVETSKLPVSKQFEEEVKEAVKRKITNGDRPQDLAKREEVMVMVKRAGEFQSYQTNELAKTFVEVKDKLPLQKPDQWEEKLKGGDITQQEVIYLMAQLTLRSFK
Other Proteins in cluster: phalp2_29138
Total (incl. this protein): 14 Avg length: 321,4 Avg pI: 9,60
Protein ID Length (AA) pI
7cEL4 378 9,31686
1d982 298 9,47867
7jTCZ 378 9,28172
A0A4D6BC49 279 9,25432
A0A4D6ALV3 279 9,35773
M1PRV7 278 9,31318
A0A9E7LK47 311 9,79857
A0A9E7RZ88 311 9,79857
A0A9Y1YVD4 349 9,83370
A0A9Y1YWK3 349 9,83370
A0AAE9K5E3 279 9,82577
A0AAE9K8C0 348 9,79425
A0AAX4ANS2 314 9,69709
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_3982
7wtdu
 333 46,7% 327 1.032E-111
2 phalp2_29544
3KzTO
 225 39,6% 250 1.799E-50
3 phalp2_8205
7kqpf
 6 37,5% 258 2.166E-45
4 phalp2_6450
8Jtqs
 137 30,3% 270 4.567E-27
5 phalp2_10688
2YkiQ
 5 31,3% 300 6.683E-26
6 phalp2_38227
7x2xX
 21 31,2% 253 9.700E-25
7 phalp2_36521
1qwup
 277 28,5% 245 1.815E-14

Domains

Domains [InterPro]
Ami2
PG_1
Unannotated
Representative sequence (used for alignment): 7cEL4 (378 AA)
Member sequence: A0A9Y1YVQ3 (348 AA)
1 378 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01471, PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Bacillus phage SPbetaL2
[NCBI] 		3053436 Spbetavirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OQ921342 [NCBI]
CDS location
range 39375 -> 40421
strand -
CDS
ATGGCTATTAAAGCGAGACAAATGTTAGTATCCCCAGAGAAATATTCAATCAAATGTCCATATGCATTATCTGCTTCATTCATCACTTTTCACAATACATACAACGACGCACCAGCGCAAAATGAAGTTAGTTATATGATCGGCAACAATAATGAAGTTTCTTTTCACTTTGCTGTAGATGATAAAGAGGTTGTTCAAGGGATTCCTACAAATCGTAATGCATGGCATACAGGTGATGGATCAGGTGTGAATTCAGGAAACCGGACTTCTATCGGTGTTGAAGTTTGCTACTCTAAATCTGGTGGAGAGCGCTATAGAAAAGCTGAAGCGTTGGCTATTAAATTTATTGCACAACTCCTTAAAGAACGTGGCTGGGGCGTGGATCGAGTTAAAAAGCATCAAGACTGGTCTGGCAAATACTGTCCGCATCGAGTTCTTGATGAAGGACGTTGGAATGCTGTTAAATCTGCTATTGCTGCTGAATTGAAAGCACTCGGTGGAAATACTTCTTCCTCATCTTCAAAGCCAACAAAAGTCGTTAAAACAAATGGGTCTTATGTTAAGAATACAGTTATCGCAGACAGTCTTAATGTGAGAACCCAGCGCAATGCAAACTCCTCTATTGTACTTACCCTCCCTAAAGGCTCCACTGTCCAATATCAAAAAGGATCAACTCAAAACGGTTGGGGATATATCAAATATACAAACTCTAAAGGTGCTACATACAGTGGTTACGTCAATGTGAAATACATTAAAAGTGATGGTGAACTTGGGCAATCAACCCCAAAGGCCAAACCCACTTCTAAGCCTAATAGCAGCGGAATCAAATCTGTAGGCGAAATTAAGATTGTCGGTGTAAAGAGCGCTGCAATCGTAATGGACAGACCGGATAAAAATAAAGCGAAGAACCTTGGCACAGTAACTCTGGGTGATACGCTCAGCATTTCTGGTTCAGTTAAAGGTAAAAACAATTCCAATGGCTACTGGGAAGTTATTTATAAAGGTAAACGTGGATATATCTCGGGACAGTTTGGATCAACAATCTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0002ece5bc_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7cEL4) rather than this protein.
PDB ID
7cEL4
Method AlphaFoldv2
Resolution 78.62
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50