Protein

Protein accession
A0A9E8K514 [UniProt]
Representative
6w5Af
Source
UniProt (cluster: phalp2_24718)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MKSKGWSKMATVNGVLFRQNLVSSSKYGIKAPNTMKPKKITVHNTYNDATSQNETDYCKNNNNEVSFHVAVDDKEAIQVVPFNRNAWHCGDGGNGYGNRNTIGVEICYSKSGGSKYIKAEQNAIKYIAGLCEQQGIAASKDTIKKHQDWSGKYCPHRILAEKRWPAMQQAIIDEYNRIKSSKSHTPASSSKNTYYTENPRKVKTLVQCDLYNSVDFTTKNKTGGTYPPGTIFTITGMAKTKGGTPRLKTKSGYYLTANTKFVKKI
Physico‐chemical
properties
protein length:265 AA
molecular weight:29537,2 Da
isoelectric point:9,70
hydropathy:-0,77
Representative Protein Details
Accession
6w5Af
Protein name
6w5Af
Sequence length
286 AA
Molecular weight
32902,60700 Da
Isoelectric point
6,53514
Sequence
MTYLFKQNLVSVSKHSIKCPNSMTPAFVVIHNTYNDASAANEVKYMISNNNQVSYHVAVDDKEVIQALPLNRNAWACGDGSNGPGNRKGISIEICYSKSGGSRYAVAEENAVYVAARLLQKYNLGIDELKQHADFANKNCPHRIRDEKRWDEFKGRVQWVLDEIKKGNIESALESGTTEIKKTAPSTVGKDQWKKNDKGWWYEYTDGSYPTNEWLKIDNEWYYFGNDGYAYCNKWVKWNDVWYWLDKDCKMVKDCILLINGKYYAFDKDGKMKTDVEVSDNGDLIL
Other Proteins in cluster: phalp2_24718
Total (incl. this protein): 15 Avg length: 267,9 Avg pI: 8,26
Protein ID Length (AA) pI
6w5Af 286 6,53514
13t9d 263 8,34203
5Orfp 263 8,33713
5SUd7 263 7,57154
5hVEd 242 9,25394
5i2SE 258 9,13802
5i4xc 258 9,16761
7dnjd 314 9,41446
7nGkl 263 5,06358
7qBiZ 319 6,46489
7qsJI 239 8,73793
8eyZS 263 8,35183
8lSG9 260 9,08916
8onZi 263 8,77339
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_16558
7rWDg
 20 46,5% 217 5.282E-64
2 phalp2_29138
7cEL4
 14 42,3% 241 9.697E-61
3 phalp2_38352
A4V0
 20 34,7% 259 5.154E-40
4 phalp2_10049
7q2PM
 57 28,9% 311 6.000E-30
5 phalp2_20231
8lqkG
 12 34,8% 218 1.294E-28
6 phalp2_38686
KaEr
 718 30,7% 263 1.505E-27
7 phalp2_5248
854PA
 247 29,1% 278 9.236E-25
8 phalp2_1064
7bl2o
 2 29,8% 275 2.305E-24
9 phalp2_30595
6aPsK
 7 28,6% 272 7.794E-24
10 phalp2_17898
EGF4
 130 31,6% 202 1.630E-22

Domains

Domains [InterPro]
Ami2
Choline_bind_3
Representative sequence (used for alignment): 6w5Af (286 AA)
Member sequence: A0A9E8K514 (265 AA)
1 286 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF19127

Taxonomy

  Name Taxonomy ID Lineage
Phage Listeria phage LP-P111
[NCBI] 		2992846 Slepowronvirus >
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OP779214 [NCBI]
CDS location
range 39507 -> 40304
strand +
CDS
ATGAAATCGAAGGGGTGGAGTAAGATGGCTACAGTTAACGGAGTACTCTTTAGACAAAATCTAGTTTCTAGTTCAAAATATGGAATCAAAGCACCTAACACGATGAAACCAAAGAAAATCACTGTCCACAACACTTACAATGATGCTACATCACAAAACGAGACAGATTACTGTAAGAACAATAACAATGAAGTTAGTTTCCACGTTGCAGTCGATGATAAAGAAGCTATCCAAGTTGTTCCTTTTAATAGAAATGCGTGGCATTGTGGCGATGGCGGAAATGGATATGGAAACAGGAATACTATCGGTGTAGAAATTTGTTACAGCAAATCTGGAGGTTCCAAATATATAAAAGCAGAGCAAAATGCAATCAAGTATATTGCAGGGCTATGCGAACAGCAAGGAATTGCGGCTTCGAAAGATACGATTAAAAAGCATCAAGACTGGAGCGGTAAATATTGCCCACATCGTATCTTAGCGGAAAAGCGTTGGCCAGCAATGCAACAAGCGATTATAGATGAATACAATCGTATTAAATCTTCTAAATCACACACGCCAGCAAGTTCAAGCAAAAACACATACTACACAGAAAACCCACGAAAAGTTAAAACACTAGTACAATGTGATCTATACAATTCAGTAGACTTTACAACAAAAAACAAAACGGGTGGCACATATCCGCCAGGCACTATCTTCACGATTACAGGAATGGCTAAAACAAAAGGCGGAACACCTCGCTTGAAGACGAAGAGCGGTTACTATCTCACTGCTAACACGAAGTTTGTTAAAAAGATTTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (6w5Af) rather than this protein.
PDB ID
6w5Af
Method AlphaFoldv2
Resolution 93.69
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50