Protein

Protein accession
A0A9E6YZE7 [UniProt]
Representative
4kiLL
Source
UniProt (cluster: phalp2_21830)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MGFELIKKITSPGQGAINPSYFVIHSTANVGATALNHVTYWTNNPNNSVHYVTDWTGKVYNCVEWNRKCWHVGGGNSYTTGIELCETNDPKQFAKEWETATEFTAWFLKQRGWGIDRLISHDDARKKWGGTDHTDPNAYFAKFGKTMNDFRNDVKKIMNGSPAKVGWINDKGRWWYRHQDGSYTKNGWELINGKWYAFDKDGWMLTGWYHPKQGEWYYLDPKNGDMSVGWRQIGNDWFYFEPSAKGKLKQGQMYADAWAKDSKGLDYWLGGDGRMAKSTWVDKARYYVNAQGVWDKSKS
Physico‐chemical
properties
protein length:299 AA
molecular weight:34608,2 Da
isoelectric point:9,03
hydropathy:-0,87
Representative Protein Details
Accession
4kiLL
Protein name
4kiLL
Sequence length
309 AA
Molecular weight
34922,25980 Da
Isoelectric point
4,92535
Sequence
MIRIRGVMNIIEDIVSWGHGELYPSYVVIHETANPGATARNHRDYWASNDTYAVHYCGDWTGDVYHCVPDDRICWQVGNGNPYVVGIELCHATNQEDFESVWRVGIEWSAMMLNRYGWGIDRLISHNDCTNWWGGSDHTDPISYFENHGKSWEQFKQEVADYMASGGFEEEAMYDVNVPAAGTPVHRLFNAGSGEHFYTCAEDEKDGLVANGWAYEGVAWRCPDPEVAVFRMWMPGGKHFFTASFDEAQGLTKNRWKCEGVPFFAKREGTPVRRAFNKYTGDHLLTTSDTDMANAIAAGYADEGVAFHV
Other Proteins in cluster: phalp2_21830
Total (incl. this protein): 4 Avg length: 313,3 Avg pI: 6,88
Protein ID Length (AA) pI
4kiLL 309 4,92535
3VGNP 329 8,82071
3yQN2 316 4,74739
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_14323
3WMg9
 12 23,5% 285 9.491E-18
2 phalp2_25741
4Lci3
 6 20,1% 218 8.079E-16
3 phalp2_32244
7rT4s
 46 26,9% 208 3.972E-12
4 phalp2_30009
2eOPi
 1 25,4% 212 5.411E-11
5 phalp2_39033
8owcJ
 65 23,3% 248 2.289E-08

Domains

Domains [InterPro]
Ami2
DUF5648
Representative sequence (used for alignment): 4kiLL (309 AA)
Member sequence: A0A9E6YZE7 (299 AA)
1 309 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF18885

Taxonomy

  Name Taxonomy ID Lineage
Phage Chaetfec virus UA24_144
[NCBI] 		2914463 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
OM154937 [NCBI]
CDS location
range 49098 -> 49997
strand +
CDS
ATGGGTTTTGAACTTATTAAGAAAATTACGTCTCCGGGTCAGGGAGCCATCAATCCGAGTTATTTTGTCATCCACTCTACGGCGAATGTCGGGGCGACCGCTCTTAACCACGTGACTTATTGGACTAACAATCCCAATAATTCAGTGCATTATGTGACTGACTGGACGGGGAAGGTCTATAATTGCGTCGAGTGGAATCGCAAGTGCTGGCATGTCGGTGGCGGCAACAGTTACACCACCGGCATCGAGCTTTGCGAGACGAACGACCCCAAGCAGTTCGCCAAGGAATGGGAAACCGCCACTGAGTTTACGGCCTGGTTCCTTAAGCAGCGCGGTTGGGGCATTGATCGTCTTATCTCTCACGATGACGCTCGTAAGAAGTGGGGCGGCACTGATCATACCGACCCCAACGCTTACTTTGCAAAGTTCGGTAAAACGATGAACGACTTCCGAAACGATGTTAAGAAGATTATGAACGGCTCGCCTGCTAAGGTCGGCTGGATCAACGACAAGGGGCGTTGGTGGTATCGCCATCAGGACGGCTCTTACACAAAGAACGGCTGGGAGCTGATCAACGGCAAGTGGTACGCGTTCGATAAGGATGGCTGGATGCTGACCGGCTGGTATCATCCTAAGCAAGGCGAATGGTACTATCTCGACCCCAAGAACGGCGACATGTCTGTTGGTTGGCGACAGATTGGCAATGACTGGTTCTACTTTGAGCCCTCTGCCAAGGGCAAGCTTAAGCAGGGCCAAATGTACGCAGACGCGTGGGCTAAGGACAGCAAGGGTCTTGATTATTGGCTCGGCGGCGATGGCCGAATGGCTAAGTCGACCTGGGTCGATAAGGCGCGCTACTACGTTAACGCTCAGGGTGTCTGGGACAAGTCTAAGAGCTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (4kiLL) rather than this protein.
PDB ID
4kiLL
Method AlphaFoldv2
Resolution 89.61
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50