Protein

Protein accession
A0A8S5USZ0 [UniProt]
Representative
8bEwv
Source
UniProt (cluster: phalp2_866)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTRIEPNWAVTDVHQSANYDEGRPAGPPTGIVIHWWGLPEWNQTHDGVVDFLCNGNRSARTSAHYVVSAGRITQIVSDNDRAWHCSGNNLRTIGIECHPACTDDDLRTVAKLVSAIRFEHGYLPLSRHCDHFPTACPGNYSDKLAEIDRIANEGEFDEMQLTDRITRPDGHNASVADILAYLDLRVERIERALLGGVEKKGSDGQPTGSMTNPLDETAWNATNFSRVYEGISALNTRLDDLAKLIEAGAK
Physico‐chemical
properties
protein length:250 AA
molecular weight:27634,4 Da
isoelectric point:5,22
hydropathy:-0,49
Representative Protein Details
Accession
8bEwv
Protein name
8bEwv
Sequence length
253 AA
Molecular weight
27800,71320 Da
Isoelectric point
5,19317
Sequence
MTIMIRPNYAVTDVHPSPNFDPGRPGGAPTGIVIHWWGLPEWGQTHDQVVAFLSDGNRANPTSAHYVVSEGRITQLVSDADLSWHCAGNNMRTIGSECHPAATDGDMKTIARLIAAIRSEWGNLPLSRHCDHYATACPGNYIDRLDALDYLSTHPEESENEMQLSDRVVRPDGHNASVNDVLAYIDMRVERLESALLGGVEKKDQDGKPTGAMTNAVDETAWNATNFARVYQRLDALTKRVNDLVSLIEVGAK
Other Proteins in cluster: phalp2_866
Total (incl. this protein): 56 Avg length: 253,8 Avg pI: 5,49
Protein ID Length (AA) pI
8bEwv 253 5,19317
11tqb 241 5,13781
11vnd 240 5,22120
1cx09 203 4,80258
3c4xU 267 6,11146
5N0A7 267 5,64169
5RVEk 250 5,52233
5U4ay 250 5,24444
5ZCMI 267 5,62526
63kOj 249 5,31339
6YdVZ 267 6,01461
6Z2wQ 250 5,14350
6Z6cm 253 5,16055
6ZfML 254 5,09371
6nTme 267 5,91634
6pqW2 250 5,44935
6uwwB 253 4,94433
70tS7 242 5,23876
7ZBjp 250 5,33266
7Zyen 240 5,17908
8bq2B 267 5,90133
8bqgy 267 5,99949
8hiRL 253 4,99560
8hlaf 242 5,43718
8kSGP 267 5,79067
8mKS9 267 5,80380
8outV 267 5,99836
8ozVr 241 5,24819
8qVt3 267 5,79067
8qVut 267 6,32438
8siFH 267 6,09424
BF5m 242 5,43718
BuSz 267 6,01097
CM5U 240 5,18181
CUr6 241 5,06466
CXvx 250 5,08257
DDlp 242 5,43718
F2an 240 5,14350
HD9e 253 5,01317
HDFN 241 5,14179
K5FK 241 5,22347
K6cM 267 6,26459
K9zX 250 5,21671
MYeQ 267 5,80766
NJQo 253 5,09382
Nrsb 256 5,79658
OIxt 242 5,43718
oDcx 267 6,02405
oLCf 250 5,22301
oNVL 267 6,01461
oNVM 267 5,59821
pFCj 267 5,91622
q1bE 250 5,31839
wHUX 267 5,80277
z6Aa 250 5,14662
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_5947
6lvSt
 2 70,9% 162 2.267E-87
2 phalp2_16559
7s5Uq
 12 38,1% 202 1.502E-47
3 phalp2_31556
4fe7g
 22 36,5% 167 4.639E-33
4 phalp2_15294
1dl82
 2 33,5% 197 2.001E-29
5 phalp2_10059
7uj9v
 1 30,0% 203 4.437E-12

Domains

Domains [InterPro]
Ami2
Unannotated
Disordered region
Representative sequence (used for alignment): 8bEwv (253 AA)
Member sequence: A0A8S5USZ0 (250 AA)
1 253 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctEZK6
[NCBI] 		2825397 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK016134 [NCBI]
CDS location
range 13281 -> 14033
strand +
CDS
ATGACACGAATCGAGCCTAATTGGGCTGTAACCGACGTACACCAATCCGCTAATTACGACGAAGGCCGTCCCGCTGGACCACCTACCGGAATCGTGATCCATTGGTGGGGACTCCCGGAATGGAATCAGACGCATGATGGAGTCGTAGATTTCCTATGCAACGGCAATCGCAGCGCGCGCACATCAGCGCACTATGTTGTCTCAGCAGGCCGTATCACTCAGATTGTCTCCGATAACGATCGTGCATGGCACTGCTCAGGTAATAACCTGCGCACAATCGGTATCGAGTGCCATCCCGCATGCACAGACGATGACCTGCGCACAGTGGCAAAGCTCGTCTCAGCGATCCGCTTTGAGCACGGCTATCTGCCCCTCTCTCGTCACTGCGATCACTTTCCGACCGCCTGTCCCGGAAACTACTCAGACAAACTTGCAGAAATCGACCGAATTGCAAATGAAGGAGAATTTGACGAAATGCAACTCACAGACCGCATCACTCGTCCCGATGGTCATAACGCTAGTGTCGCAGACATTCTTGCCTATCTTGATCTGCGCGTGGAGCGCATCGAGCGCGCACTACTCGGCGGTGTCGAAAAAAAGGGCTCAGATGGTCAGCCCACAGGATCGATGACTAACCCCCTCGATGAAACCGCGTGGAATGCAACGAATTTCAGCCGCGTTTACGAAGGCATTTCAGCTCTCAATACGCGTCTCGACGATCTTGCAAAGCTGATAGAGGCAGGTGCGAAGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8bEwv) rather than this protein.
PDB ID
8bEwv
Method AlphaFoldv2
Resolution 87.58
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50