Protein

Protein accession
A0A8S5URH6 [UniProt]
Representative
2TxWe
Source
UniProt (cluster: phalp2_14206)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MNSSLVNYTKLSPNYNVRDRKISKITIHHMAGNLTVEICGNIFASSAREASANYGIGSDGRVGLYVEEKNRSWASANRENDMMAVTIEVANDGGAPDWHVSNTAIAKLIELCTDICKRNGIEKLNYTGDTSGNVTRHNMFVPTNCPGPYLQSKFPYIVSEVNKRLCANEEMYRIRKSWVDAKSQIGAYRNLENAKKVCPSGYYVFNSAGKVVYPVVSNDSIDYAQSFDPSKAGSYTVVPSVGLHLRAGAGTDKRSLQVLEQGAVVQCYGYYTGQWYLVQTSDGKVGYVCSEYLK
Physico‐chemical
properties
protein length:294 AA
molecular weight:32461,2 Da
isoelectric point:8,64
hydropathy:-0,37
Representative Protein Details
Accession
2TxWe
Protein name
2TxWe
Sequence length
346 AA
Molecular weight
38295,78580 Da
Isoelectric point
9,19714
Sequence
MSNSSLVSFTRISPNYSNRTSAIKKITIHHMAGNLSVETCGNVFASSARQASANYGIGSDGRVGMYVEEKFKPWTSSNHNNDNQAVTIEVANSKGAPNWEVSDKALSKLIELCVDICKRNGIAKLNFTGDKSGNLTMHSMFAATACPGPYLKSKFGYIAEQVNANLNQPTSQQVSGKVKIKQGATDLNSGRQFSSFVYSEVYDVIKVDGSKVKFGKGTAVTGVTDISNIIGYVQPAEKPIIPTNTQPIYKLGQYLSNGIDYGYVFDHEYYSNNYPDLKAAFGMDKTKLFNHFLVNGMKEERIANPYFDVKVYRAMYSDLRSVFGNNTIKYYEHFCKYGAKENRAAK
Other Proteins in cluster: phalp2_14206
Total (incl. this protein): 30 Avg length: 302,8 Avg pI: 7,52
Protein ID Length (AA) pI
2TxWe 346 9,19714
14NRz 273 7,63094
21pTH 273 5,97318
232M9 413 8,99033
3GQC7 312 8,65857
3TH5j 273 8,11310
3TPJ1 276 6,52622
3TQ7u 247 6,98747
3VI5v 384 6,00682
3Zycn 302 7,47571
3fWmQ 410 8,39116
3gIaH 273 8,04180
3h3HN 273 6,12778
3wann 316 4,98787
40lMS 288 6,10283
41iA7 273 7,62628
4JZHd 273 8,45111
4L0Pl 273 7,60860
4L1cP 273 8,07758
5RMwe 301 9,67472
5USKE 316 5,15356
5WsGq 302 9,17329
66v1a 317 5,07631
67Bpg 337 9,03661
6wyeA 233 6,30261
7XuOl 312 8,65857
87yzE 316 4,76887
8rye8 294 9,43309
OGze 312 8,65857
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_37526
3iAYu
 8 41,0% 351 1.806E-96
2 phalp2_37208
21ki5
 1 32,7% 370 1.544E-83
3 phalp2_18247
7DM9M
 20 34,8% 327 3.535E-82
4 phalp2_12971
3esnk
 626 50,4% 222 1.177E-75
5 phalp2_32522
1n71i
 16 39,7% 259 3.522E-58
6 phalp2_21398
41bpM
 1 25,8% 298 7.891E-30

Domains

Domains [InterPro]
Ami2
Unannotated
Unannotated
Representative sequence (used for alignment): 2TxWe (346 AA)
Member sequence: A0A8S5URH6 (294 AA)
1 346 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctg6c78
[NCBI] 		2825603 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK016125 [NCBI]
CDS location
range 30699 -> 31583
strand +
CDS
ATGAACAGTTCTTTAGTTAATTACACAAAACTATCTCCAAACTATAATGTTAGAGATAGAAAAATATCAAAAATTACAATTCATCATATGGCTGGAAATTTAACCGTAGAAATATGTGGTAATATTTTTGCTTCAAGTGCAAGAGAAGCCAGTGCTAATTATGGTATTGGGTCAGATGGCAGAGTCGGATTGTATGTAGAAGAAAAGAATCGTTCTTGGGCAAGTGCAAATAGAGAAAATGATATGATGGCCGTGACAATTGAAGTAGCGAATGATGGTGGAGCGCCAGATTGGCACGTGAGTAATACGGCTATTGCAAAATTAATTGAACTTTGTACAGATATATGTAAAAGGAATGGAATTGAGAAGTTAAATTATACTGGTGATACTAGCGGTAATGTTACAAGGCATAATATGTTTGTTCCAACTAATTGCCCAGGGCCTTATTTACAAAGTAAGTTTCCATATATAGTTAGCGAAGTAAATAAAAGACTTTGCGCAAACGAAGAGATGTATAGAATTAGAAAATCTTGGGTAGATGCTAAGTCTCAAATTGGGGCATATAGAAATTTAGAGAATGCTAAAAAGGTTTGCCCTAGTGGATATTATGTTTTTAACAGTGCTGGCAAAGTAGTTTATCCAGTTGTATCAAACGATTCTATTGATTATGCACAAAGTTTTGATCCATCTAAGGCGGGAAGTTATACAGTTGTTCCGTCAGTGGGATTACATTTGAGAGCTGGGGCTGGTACAGATAAAAGAAGCCTTCAGGTGCTTGAACAAGGGGCTGTTGTTCAATGTTATGGCTATTATACAGGCCAATGGTATCTTGTTCAAACATCCGATGGCAAAGTCGGTTATGTTTGTAGTGAATATTTAAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (2TxWe) rather than this protein.
PDB ID
2TxWe
Method AlphaFoldv2
Resolution 87.91
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50