Protein

Protein accession
A0A8S5UPZ1 [UniProt]
Representative
7gerf
Source
UniProt (cluster: phalp2_36859)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MQMRTKKNKLLKRGRAVALAAVLFCTLILGTMAVVPPIRAEAAPINGLPINQKLQTINASSRYGNGIKYIVVHYTGAPGSAANNATYFSTGYRGASAHYFVGYSGEVWQSVSDSLAAWSVGGNKYPGTKGGSVYGKCTNYNSINIEMCVRTSGSRSDTSKDWYFENATINSTVKLVQGLMKKYNVPLSRVVRHYDVVGKYCPNPFVLNDDPVTWSSFKSMVAGDKDLPPDTGSSSTSTKPSKPSTGGSVSSSGINVRYQAYVNGQWLPWVTNYNNVSSDGYAGIPCRAVTGLRAYTVGSQSAVGNLQYRVHLRGGQWLPWVTDASGKAPNDYAGIYGHVIDGIQVKLVNKPGYHAEVRVQLTDRTGWLSWSSQYSTGADAYAGIYGIGIDRVQIRIVKN
Physico‐chemical
properties
protein length:399 AA
molecular weight:43183,3 Da
isoelectric point:9,65
hydropathy:-0,27
Representative Protein Details
Accession
7gerf
Protein name
7gerf
Sequence length
514 AA
Molecular weight
56133,86470 Da
Isoelectric point
9,56145
Sequence
MVEAMQLDIFKMVMENLNLLIFVAGVICFLTVVITQVTKGLPGLRSIPTDLQVLVLSVVFTLVAYFAYVDMTGFPVTWYFVIAMIVIGFVLALVATKGWTYVADLFWRFQHKGMMQMRTKKNKLLKRGRAVALAAVLFCTLIFGTMAVVPPIRAEAASINGLPINQKLQTINASSRYGNGIKYIVVHYTGAPGSAANNATYFSTGYRGASAHYFVGYSGEVWQSVSDSLAAWSVGGNKYPGTKGGSVYGKCTNYNSINIEMCVRTSGSRSDTSKDWYFENATINSTVKLVQGLMKKYNVPLSRVVRHYDVVGKYCPNPFVLNDDPVTWSSFKSMVAGDKDLPADTGGSVNTSTKPSKPSTGGSVSSSGINVRYQAYVNGQWLPWVTNYNNVSSDGYAGIPCRAITGLKAYTVGSQSAVGNLQYRVHLRGGRWLPWVTDASGKAPNDYAGIYGHVIDGIQVKLVNKPGYHAEVRVQLTDRTGWLSWSSQYSTGADAYAGIYGIGIDRVQIRIVKN
Other Proteins in cluster: phalp2_36859
Total (incl. this protein): 18 Avg length: 401,7 Avg pI: 9,61
Protein ID Length (AA) pI
7gerf 514 9,56145
2lsFM 397 9,65048
3jLC9 396 9,61606
3kgAd 397 9,65048
3qQ4i 396 9,71411
3qUgo 397 9,63875
3uOe0 397 9,62263
3wtkg 397 9,61090
5XF7E 368 9,41479
5YD8v 400 9,61090
66gAT 400 9,65048
6w9jJ 400 9,67685
79Fmy 398 9,65048
7gerj 397 9,63856
7muxk 383 9,47287
8lqc2 397 9,62263
OGDl 397 9,61070
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_20231
8lqkG
 12 44,0% 347 2.359E-71
2 phalp2_36721
6gIHr
 182 37,7% 352 1.244E-43
3 phalp2_28981
6d4eV
 350 30,9% 372 6.349E-28
4 phalp2_8364
oq39
 14 27,6% 365 2.680E-26
5 phalp2_5505
3kRYJ
 67 28,7% 351 5.890E-22
6 phalp2_1330
13aRO
 64 27,0% 347 1.771E-20

Domains

Domains [InterPro]
Unannotated
Ami2
Unannotated
Representative sequence (used for alignment): 7gerf (514 AA)
Member sequence: A0A8S5UPZ1 (399 AA)
1 514 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ct5FX1
[NCBI] 		2825335 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK016115 [NCBI]
CDS location
range 1719 -> 2918
strand +
CDS
ATGCAGATGCGGACGAAGAAGAATAAATTACTGAAACGAGGAAGGGCGGTTGCGTTGGCAGCCGTCCTTTTCTGTACCCTGATTTTGGGGACAATGGCCGTGGTGCCGCCGATCCGGGCGGAGGCGGCGCCCATCAACGGATTGCCGATCAACCAGAAGCTTCAGACCATCAATGCGTCCAGCCGGTACGGGAACGGGATCAAGTACATCGTAGTGCATTATACCGGGGCTCCGGGAAGTGCCGCAAACAATGCGACCTATTTTTCAACTGGATACCGTGGGGCGTCCGCCCACTATTTTGTCGGGTATTCCGGAGAGGTCTGGCAGAGTGTGTCTGACAGTCTGGCGGCCTGGTCGGTAGGCGGCAACAAATACCCAGGCACAAAGGGCGGAAGCGTCTATGGGAAATGCACCAACTACAACAGCATCAATATTGAGATGTGTGTAAGGACGTCCGGGAGCCGGAGTGATACGTCCAAGGACTGGTACTTTGAAAACGCCACGATCAACAGCACAGTGAAGCTGGTGCAGGGGCTGATGAAGAAGTACAATGTTCCCCTGTCCCGTGTGGTTCGGCACTATGATGTGGTCGGGAAATACTGTCCGAATCCGTTTGTACTGAACGACGACCCGGTAACATGGAGCTCTTTCAAGAGCATGGTAGCCGGGGACAAAGATCTGCCGCCGGATACGGGAAGTTCCAGTACATCCACAAAACCGTCCAAACCGTCCACTGGCGGGTCCGTATCCTCATCCGGAATCAATGTCCGGTATCAGGCATATGTGAATGGCCAGTGGCTTCCGTGGGTGACAAACTATAATAATGTCAGTTCCGACGGCTACGCAGGGATTCCGTGCAGGGCGGTCACCGGACTGAGAGCGTATACAGTTGGCTCCCAGTCCGCAGTTGGAAATTTACAGTACCGGGTACACTTGAGGGGAGGCCAGTGGCTGCCTTGGGTGACGGACGCATCCGGAAAGGCTCCGAATGATTACGCCGGGATCTATGGACACGTCATCGACGGAATCCAGGTAAAGCTTGTCAATAAACCGGGATATCATGCGGAGGTACGAGTACAGCTCACCGACCGAACCGGATGGCTTTCCTGGTCCAGCCAGTACAGCACGGGCGCGGATGCCTACGCCGGGATCTATGGGATCGGGATTGACCGGGTACAGATACGGATTGTAAAAAATTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7gerf) rather than this protein.
PDB ID
7gerf
Method AlphaFoldv2
Resolution 78.70
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50