Protein

Protein accession
A0A8S5UL90 [UniProt]
Representative
1njYk
Source
UniProt (cluster: phalp2_9579)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MALKINDTIRATRVGGKRPISAIRAIVFHYTANTGLHATALGNARYFANGSEGRPASAHFVVDEGDTVYQCVPLNVVAWAVGDGRSGKFGKVYSNYNTVSIEMVSHTDASGKYYIPEATMRNAARLYQMLLKRLPGVQAAIRHYDISMKLCPLPLIDEKKWADFKKLLEEVDEVVTKAKMIIDGKEIEVERILKDGTNYIKIRDIAKALDLEISNKGNVPILNHKGG
Physico‐chemical
properties
protein length:227 AA
molecular weight:25118,7 Da
isoelectric point:9,28
hydropathy:-0,20
Representative Protein Details
Accession
1njYk
Protein name
1njYk
Sequence length
240 AA
Molecular weight
N/A Da
Isoelectric point
7,63106
Sequence
MALKINDTIRATRVGGKRPLSAIRAIVFHYTANTGQHATALGNARYFANGSEGRAASAHFVVDEGDTVYQCVPLDVVAWAVGDGRSGKFGKVYGNYNTVSIEMVSHTDASGKYYIPEATMRNAARLYQMLLKRLPGVQAAIRHYDISMKLCPLPLIDEKKWADFKKLLEEVDEVITKSKMIIDGKEIESCGDTCIFMQDGLNPEVDFTEEAIQXXXXLKGRHELHQNSRYCKGARSGCVE
Other Proteins in cluster: phalp2_9579
Total (incl. this protein): 79 Avg length: 227,9 Avg pI: 8,71
Protein ID Length (AA) pI
1njYk 240 7,63106
1jBtS 232 6,32740
1jDex 226 8,54105
1jHJW 226 8,24030
3lXSv 232 9,28282
3shv1 232 9,20778
3tnlk 232 8,32482
3tpzp 231 8,35235
3uPRt 227 9,17709
3ukNk 178 9,34200
3wiej 232 9,29887
5JUsV 227 9,17709
5KlbF 232 9,36534
5LdFI 233 8,88595
5Lx9B 227 9,19050
5MEud 226 9,16259
5Mdt3 226 9,03868
5N8PI 226 8,61770
5NPbD 227 9,10811
5NVPY 227 9,29926
5SCik 227 9,19050
5SDrT 227 8,71775
5Sms0 232 6,61108
5VEb9 232 6,60972
5VP8k 227 9,21074
5Xg3U 229 9,08310
5XsXY 231 7,73013
5YDMQ 227 9,15324
5YoEn 233 8,88595
60JpX 226 8,71730
60a8Q 232 8,32482
61CrR 230 8,91148
61HIm 226 9,05247
62QSP 230 8,91103
62izd 230 8,91148
65Yug 227 9,16542
69VdC 226 9,17728
6YBCt 231 6,66582
6b6zG 226 8,70538
6c0Uh 225 8,73767
6eBtl 227 8,89852
6ed57 232 6,60847
6gUQ7 227 9,03726
6h6ag 226 8,89414
6h6ek 226 8,91129
6hNuI 233 8,41978
6hUM2 233 9,05247
6ianT 233 8,70538
6kZ4t 231 6,66945
6mmh7 226 9,05247
6pRh3 233 8,70538
6pVjH 225 9,17728
6px6v 233 8,71685
6qP8s 232 7,01964
6rV75 213 8,42281
6uEKf 227 9,05247
6uaFr 227 9,28301
6v7ED 226 9,17709
6v81D 225 8,86268
6w5ja 226 9,05228
6wcHE 227 9,20236
7YZ34 227 9,28617
7YZl7 225 8,90439
81Aiv 235 8,91155
81wwW 225 8,75650
83vgE 226 9,06646
87oB6 227 9,21074
87xbZ 231 7,73098
8bY1C 227 9,29984
8bYLb 228 9,31383
8loTh 231 9,19224
8pUX6 226 9,10856
8qrEl 226 9,19231
8r01B 228 9,37907
8rZQp 226 9,32646
Orz3 227 9,19231
A0A8S5M541 227 9,28301
A0A8S5MVI0 233 6,60733
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_29669
8sfID
 1005 42,8% 168 5.997E-31
2 phalp2_26828
3TOBT
 44 37,8% 190 6.312E-29
3 phalp2_11676
1LRu7
 24 39,6% 169 1.909E-27
4 phalp2_1605
81mMl
 270 41,8% 160 4.993E-25
5 phalp2_3793
5X3JD
 59 39,2% 176 9.259E-25
6 phalp2_24299
3nIle
 36 36,3% 168 2.753E-23
7 phalp2_29517
21wOt
 7 35,0% 171 5.977E-22
8 phalp2_23554
7slSx
 8 38,0% 163 8.106E-20
9 phalp2_31053
1GV4E
 13 30,9% 197 9.368E-19
10 phalp2_5052
1q1Q3
 64 37,1% 167 1.272E-18

Domains

Domains [InterPro]
Ami2
Disordered region
Representative sequence (used for alignment): 1njYk (240 AA)
Member sequence: A0A8S5UL90 (227 AA)
1 240 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctOH142
[NCBI] 		2825478 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK016107 [NCBI]
CDS location
range 5308 -> 5991
strand -
CDS
ATGGCACTGAAAATTAACGATACCATCCGGGCAACGAGAGTGGGCGGCAAGCGTCCGATCTCGGCTATCCGGGCAATCGTGTTCCACTACACGGCCAATACCGGCCTGCACGCGACGGCGCTCGGCAATGCCCGATACTTTGCCAACGGCTCCGAGGGACGCCCCGCGTCGGCACATTTCGTGGTAGACGAGGGCGATACCGTTTACCAGTGTGTGCCACTGAACGTGGTGGCGTGGGCGGTTGGCGACGGCAGGAGCGGCAAATTCGGCAAGGTGTATAGCAACTACAACACCGTCAGTATCGAGATGGTGAGCCACACGGACGCTTCTGGCAAGTATTACATTCCGGAAGCGACGATGCGCAACGCTGCGCGGCTCTATCAGATGTTGCTGAAGCGGCTGCCGGGCGTGCAGGCCGCAATCCGGCACTACGACATTTCGATGAAACTGTGTCCGCTGCCGCTGATTGACGAAAAGAAATGGGCGGACTTTAAGAAGCTCTTGGAGGAGGTGGACGAAGTGGTAACAAAGGCAAAGATGATCATTGACGGAAAAGAGATCGAGGTCGAACGGATCTTAAAGGACGGTACGAATTATATTAAAATTCGCGATATTGCAAAGGCGCTGGATCTCGAAATATCGAACAAGGGCAATGTCCCGATCCTGAATCACAAAGGAGGCTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (1njYk) rather than this protein.
PDB ID
1njYk
Method AlphaFoldv2
Resolution 80.06
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50