Protein

Protein accession
A0A8S5UC81 [UniProt]
Representative
66qOT
Source
UniProt (cluster: phalp2_237)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MPNYTNSSLVNYTKISPMQSGSRVNSRYNPSGKITVITIHHMAGNLSVETCGNVFQTREASANYGIGSDGRVGMYVEEKDRAWTSSSPSNDYKAVTIEVANCAYGDPWPVSSAAYEKLILLCADICKRNGIEKLNYTGDTTGNLTMHCWFADTACPGPYLKARFSDIANRVNAILDASKPDPTPVPSGNLAVGDVVTFLGGSHYYSSDASSPASTDLKPGKAKVTAIYNSGKHPYHLIHTDSSTSVYGWVDTSAIKELEEKPSTPSTGSYSVGDVVKFKGGNHYSSSNASSPASTGLREGPAKITYRANGAHPYHLVHTDNQTSVYGWVDASQIEGASSAPSTPSVEKFNKGDKVKIKSSTTRWVGGASIPSWVKNSQLYICGFRDSDPCVTINSDLSGVTGVLKTSDLYHN
Physico‐chemical
properties
protein length:412 AA
molecular weight:44120,5 Da
isoelectric point:7,60
hydropathy:-0,42
Representative Protein Details
Accession
66qOT
Protein name
66qOT
Sequence length
412 AA
Molecular weight
44055,60550 Da
Isoelectric point
8,44125
Sequence
MPNYTNSSLVNYTKISPMQSGSRVNSRYNPSGKITVITIHHMAGNLSVETCGNVFQTREASANYGIGSDGRVGMYVEEKDRAWTSSSPSNDYKAVTIEVANCAYGDPWPVSSAAYEKLILLCADICKRNGIEKLNYTGDTKGNLTMHCWFAATACPGPYLKARFSDIANRVNAILDASKPDPTPVPSGNLAVGDVVTFLGGSHYYSSDASSPASTDLKPGKAKVTAIYKSGKHPYHLIHTDSSTSVYGWVDASAIKELEEKPSTPSTGSYSVGDVVKFKGGNHYSSANASSPASTGLREGPAKITYKANGAHPYHLVHTDNQTSVYGWVDASQIEGASSAPSIPSVEKFNKGDKVKIKSSTTRWVGGASIPSWVKNSQLYICGFRDSDPCVTINSDLSGVTGVLKTSDLYHN
Other Proteins in cluster: phalp2_237
Total (incl. this protein): 25 Avg length: 387,4 Avg pI: 8,61
Protein ID Length (AA) pI
66qOT 412 8,44125
19e9j 303 9,19978
1gHro 301 9,14279
3lAFp 376 9,08928
3nLFE 414 9,55616
5OPg2 412 8,01170
5TxRL 429 8,93965
5WbZ1 412 8,78306
64I79 376 8,93624
6bIh6 393 9,18019
6eSiV 395 7,60536
6geK4 378 9,10966
6gpLp 376 9,07568
6grHw 412 7,59974
6jnGU 395 8,72955
6lIPU 395 7,16719
6sOvo 412 7,60571
7pdok 376 9,08928
82sAR 341 6,34195
84Odk 376 8,76398
8fPja 362 9,41653
8m2qZ 376 9,13609
8rMt3 360 9,53418
og3y 491 9,11662
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_33293
64Cdz
 41 45,3% 364 1.324E-90
2 phalp2_30077
2WEC1
 850 50,3% 284 2.804E-78
3 phalp2_15173
ovmV
 70 44,2% 285 1.175E-76
4 phalp2_5505
3kRYJ
 67 42,6% 298 2.204E-64
5 phalp2_4373
2br9t
 1 28,2% 510 7.527E-51
6 phalp2_30609
6pt95
 5 35,2% 309 7.503E-49

Domains

Domains [InterPro]
Ami2
Unannotated
Unannotated
Unannotated
Representative sequence (used for alignment): 66qOT (412 AA)
Member sequence: A0A8S5UC81 (412 AA)
1 412 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctgN495
[NCBI] 		2825608 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK016063 [NCBI]
CDS location
range 111306 -> 112544
strand -
CDS
ATGCCTAATTACACAAATAGCTCATTAGTTAACTACACTAAAATCAGCCCTATGCAGAGTGGAAGCAGAGTAAATTCCAGATACAATCCTTCTGGTAAGATTACTGTTATCACTATTCATCATATGGCCGGTAACCTTTCTGTAGAAACTTGCGGAAATGTATTCCAGACAAGAGAAGCATCTGCGAACTATGGTATTGGTTCTGACGGTCGTGTAGGAATGTATGTAGAAGAAAAGGATAGAGCTTGGACCTCGTCTAGCCCTTCAAACGATTATAAGGCAGTTACAATCGAGGTAGCTAACTGTGCATATGGTGATCCTTGGCCTGTTTCTTCTGCTGCATATGAGAAGCTGATCCTCCTTTGCGCAGATATTTGCAAACGTAATGGAATTGAGAAGCTGAACTACACTGGAGACACTACAGGTAATCTTACAATGCACTGCTGGTTTGCTGATACTGCATGCCCCGGTCCTTATCTAAAGGCAAGATTCAGCGATATCGCAAATAGAGTAAATGCTATCTTAGACGCTTCAAAGCCTGATCCGACTCCTGTACCGTCAGGTAATCTAGCTGTTGGAGATGTAGTTACCTTCCTCGGAGGTTCCCATTATTACAGTTCAGATGCTTCTTCTCCTGCGTCCACAGATCTAAAGCCAGGTAAGGCTAAGGTTACAGCTATTTACAATAGTGGAAAGCATCCTTATCACCTTATTCACACAGATAGCTCAACTTCTGTATATGGTTGGGTAGATACATCAGCAATCAAAGAACTCGAGGAGAAGCCGTCTACTCCGTCAACAGGTTCTTATAGTGTTGGTGATGTTGTAAAGTTCAAGGGCGGTAATCACTATTCAAGCTCAAATGCTTCTTCTCCTGCATCTACCGGCCTAAGGGAAGGTCCTGCAAAGATCACGTATAGGGCTAACGGAGCACATCCTTATCACCTTGTTCACACAGACAATCAAACAAGCGTATATGGTTGGGTTGATGCTTCTCAGATCGAAGGCGCTTCTTCAGCACCTTCTACCCCTTCTGTTGAAAAATTCAATAAGGGAGATAAAGTAAAGATCAAGTCGTCTACAACTAGATGGGTTGGCGGTGCGTCAATTCCTTCTTGGGTTAAGAATAGTCAGCTTTACATTTGTGGATTCAGAGATAGTGATCCTTGCGTCACAATCAATTCTGATCTATCAGGTGTAACAGGTGTTCTTAAGACTTCTGATCTTTATCACAACTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (66qOT) rather than this protein.
PDB ID
66qOT
Method AlphaFoldv2
Resolution 85.47
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50