Protein

Protein accession
A0A8S5TSS3 [UniProt]
Representative
wTKY
Source
UniProt (cluster: phalp2_6494)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MTITQKLLTPNEYSRPQAPIKELRGIVIHWTANPHASAEQNRLFFEAKKTGMGGYGSAHYIIGQDGEVLQIIPTNEIAYHCGTNQKDPASGQFYTDYARRKYGHYAVHWQTTSPNYCTIGIELCPTDYDGNFTEKTINAAIDLCAYLCKRHNLAADDITTHHDIVGWKDCPRLWTRKPELLDAFRASVKDAITRGQA
Physico‐chemical
properties
protein length:197 AA
molecular weight:22230,8 Da
isoelectric point:7,10
hydropathy:-0,53
Representative Protein Details
Accession
wTKY
Protein name
wTKY
Sequence length
127 AA
Molecular weight
14107,87230 Da
Isoelectric point
7,18873
Sequence
CIPENEIAYHCGSNKLDPVSGRTYTDEARKRFGHYALRPAVTSPNYVTLGVELCPIDDAGHFSDATIVSAVELCAKLIKRHKLTTDDITTHHNIVGWKNCPKLWTDHPDLFKAFKASVADFLARGNV
Other Proteins in cluster: phalp2_6494
Total (incl. this protein): 6 Avg length: 173,2 Avg pI: 7,47
Protein ID Length (AA) pI
wTKY 127 7,18873
4H7IT 164 6,50348
4qFIe 194 7,11859
5B57u 164 8,73168
A0A8S5TT50 193 8,18563
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_31879
4GdWf
 8 36,6% 120 6.077E-23
2 phalp2_15277
16VLX
 4 25,8% 120 1.111E-12
3 phalp2_17069
2aUVg
 3 30,4% 128 3.905E-12

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): wTKY (127 AA)
Member sequence: A0A8S5TSS3 (197 AA)
1 127 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ct8aS59
[NCBI] 		2825365 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015922 [NCBI]
CDS location
range 19131 -> 19724
strand -
CDS
ATGACAATCACACAAAAATTATTAACGCCGAACGAATACAGCAGACCGCAGGCACCAATAAAGGAACTGCGCGGCATTGTAATTCATTGGACGGCAAACCCACACGCGAGCGCGGAACAAAACCGCCTGTTTTTTGAAGCAAAAAAAACGGGCATGGGCGGCTATGGTTCGGCTCATTACATCATCGGACAAGACGGCGAGGTTTTACAGATAATCCCGACGAATGAAATAGCGTACCATTGCGGAACGAATCAGAAAGACCCAGCAAGCGGGCAGTTTTACACCGACTACGCCCGCAGGAAATACGGACATTATGCCGTACACTGGCAGACCACAAGCCCGAACTATTGCACAATTGGGATAGAACTTTGTCCGACCGACTACGACGGCAATTTTACGGAAAAAACAATAAACGCCGCCATTGATTTGTGCGCCTACCTATGCAAGCGGCACAACCTCGCGGCAGACGACATCACGACACATCACGACATCGTGGGCTGGAAAGACTGCCCGCGCCTATGGACTAGAAAGCCCGAACTATTAGACGCTTTCCGCGCAAGCGTAAAAGACGCAATCACAAGGGGGCAAGCATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (wTKY) rather than this protein.
PDB ID
wTKY
Method AlphaFoldv2
Resolution 96.98
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50