Protein

Protein accession
A0A8S5TBE5 [UniProt]
Representative
FRHW
Source
UniProt (cluster: phalp2_8397)
Protein name
Endolysin
Lysin probability
100%
PhaLP type
endolysin
Probability: 97% (predicted by ML model)
Protein sequence
MQGEEVRTSDKGIHLMHHFEGYRSRPYKCSARIWTVGWGHAMYADQLRLPNARSESYKGMIRDEYQLRQEDDRIWSKEELVEIFKNDIASFERGVLRLVPGVAGRQGAFDALVSFAFNAGLGNLQRSQIRIKANRGDWQGAADALMDWTKGGGKVLPGLVKRREAERALFLSGIE
Physico‐chemical
properties
protein length:175 AA
molecular weight:19884,4 Da
isoelectric point:9,30
hydropathy:-0,54
Representative Protein Details
Accession
FRHW
Protein name
FRHW
Sequence length
306 AA
Molecular weight
34456,69350 Da
Isoelectric point
6,35064
Sequence
MLSLFSTLGGLLISGLPKLLDYFQNKADQKHELALANIQMQMQLQMAAQGFAAQERMEEIRTDQIAMETDAQMTVAAYDHDKKIMDNASTWVVNFVGTVRPMVTYIFVLELCAINAWIAYYVYSRPSLVMSMEDLIRLSDIIFSSDEMAMLGGGYRTKPYLCPAQIWTIGYGHVLYQDQIRLPVARVEGKETPMIRKEMPLKPEDNRVWTKEEIYKIFDNDIAIFERGVLRLAPAVSGRQGAFDACVSFSFNAGLGGFQRSSIRMKINRGDWEGAADALLLYCMGGGKILAGLKKRRDAEKALFLS
Other Proteins in cluster: phalp2_8397
Total (incl. this protein): 6 Avg length: 189,8 Avg pI: 8,98
Protein ID Length (AA) pI
FRHW 306 6,35064
A0A6J5LS01 168 9,78638
A0A6J5LTT0 157 9,34929
A0A6J5MD79 177 9,60168
A0A6J5MQZ6 156 9,47435
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_3550
4CbTI
 96 36,5% 200 2.557E-29
2 phalp2_33546
e1Sc
 2 27,3% 307 1.141E-26

Domains

Domains [InterPro]
Disordered region
Unannotated
GH24
Representative sequence (used for alignment): FRHW (306 AA)
Member sequence: A0A8S5TBE5 (175 AA)
1 306 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF00959

Taxonomy

  Name Taxonomy ID Lineage
Phage Podoviridae sp. ctwJH20
[NCBI] 		2827753 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK032792 [NCBI]
CDS location
range 3330 -> 3857
strand +
CDS
ATGCAAGGTGAAGAAGTGAGAACATCAGACAAGGGAATCCATCTGATGCATCATTTTGAGGGCTACAGAAGTAGGCCATATAAATGCAGCGCCAGAATTTGGACTGTTGGATGGGGCCATGCGATGTATGCAGATCAGCTAAGACTGCCCAATGCAAGAAGCGAGTCCTACAAGGGGATGATTCGTGACGAGTATCAACTACGTCAAGAAGATGACCGTATCTGGTCAAAAGAAGAATTGGTTGAGATATTCAAGAATGACATCGCAAGTTTTGAACGCGGTGTTTTACGTCTTGTTCCCGGCGTGGCTGGGCGTCAAGGCGCTTTCGACGCTCTGGTCTCTTTTGCCTTCAATGCTGGGCTAGGAAACCTGCAGCGCAGTCAGATCCGCATCAAGGCCAATCGCGGTGACTGGCAAGGGGCGGCTGACGCGCTGATGGACTGGACTAAGGGTGGCGGCAAGGTGCTTCCCGGCCTGGTCAAGCGCCGGGAAGCAGAGCGGGCCTTATTCCTCTCCGGGATTGAGTAG

Gene Ontology

Description Category Evidence (source)
GO:0003796 lysozyme activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0016998 cell wall macromolecule catabolic process biological process None (UniProt)
GO:0030430 host cell cytoplasm cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0044659 viral release from host cell by cytolysis biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.2.1.17 None Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00000632

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (FRHW) rather than this protein.
PDB ID
FRHW
Method AlphaFoldv2
Resolution 83.72
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50