Protein

Protein accession
A0A8S5SXN8 [UniProt]
Representative
8dsHX
Source
UniProt (cluster: phalp2_872)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 94% (predicted by ML model)
Protein sequence
MSNSNLVTRWWPANPTNYTGGREGNKIQGIVVHHAASTSLDSIGAAFARAGRNGSAHYGVGGNQIHQYVDEANTTWHCSNWWGNVRTVGIETTNSTGAPNWEIAPDTFETLVKLVADIAKRNNLGKLWINPKADMPVLSGHKDWYGASTVCPGPSLYPRLQEIADRANAINFPPVVEVKPMIVWVNIEPLVLKAKAKVDIIDLDTGADKGDIQAGMVISNLVQETTVNGKKYYRTLYSKTKGFNNGILAEALEPMVAPVSPDSVINGGVKETKPEIKVSTEEEKRPITEKEVKEEAKKDDGFGDDIAPVPGVKPGMKPEEVQKLIEEQKKNMKIAEEIIDEVNKDMEFSPKTKMIVYLLGDLLIIAGTQAPLVVALVNTTDAVTFGQLLGQLLLSTGTMLLFTFKLLKKKGEKNV
Physico‐chemical
properties
protein length:415 AA
molecular weight:45342,4 Da
isoelectric point:6,15
hydropathy:-0,27
Representative Protein Details
Accession
8dsHX
Protein name
8dsHX
Sequence length
407 AA
Molecular weight
45030,16580 Da
Isoelectric point
5,07813
Sequence
MSQSSMVTRQWWANATNYTVGRQDKIRGIVIHHAASTSLDSIGQVFSQPGRGGSAHYGVGGKQVHQYVREEDTAWHCSNWAGNSATIGIETTNSTGAPDWKVSDETFDTLCKLVAEIAKRNGLGKLWINPDADFPALSGHKDWQGAATVCPGPYLYPKLQEICDRANEINYPTKKAELVWTKFEKVETYITTRTTHLYDFNHTHAENIVVIKEFAKDTAIDIYGKVENKTIGKTFLLTEYSFTKQITNGFKDDALTPKPAPEPAPQPQPAPEAPETPQEEPKDPEPVIEPVEPENPQEQPVISPEDNQSGLTEEELAKIQAQMQEIENNAKEKIVMIPMSNKTYDILKIVAIVILPLIQVLYTGLAKIWGFGFGTEIDQTIQLVIAAINVVLGAALVKSSSDYHNGD
Other Proteins in cluster: phalp2_872
Total (incl. this protein): 35 Avg length: 374,8 Avg pI: 5,89
Protein ID Length (AA) pI
8dsHX 407 5,07813
1gIRX 297 5,04625
21sYe 318 8,95358
23Szd 318 9,17006
23zU0 317 9,11340
3TDOv 392 5,46475
3TFR9 401 5,80260
3TIJ2 300 5,18499
3TMvt 350 4,99822
3TOWi 350 4,89323
3ZPpd 389 5,94203
3cghR 412 5,78208
3e0vQ 387 6,32569
3ePeS 392 5,56382
3fQdn 323 7,60980
3gfVM 321 4,64849
3h3fV 302 5,48635
41cYG 300 5,38341
4FWPr 294 5,07131
4UPDQ 401 5,60065
5LLJl 393 5,73354
5VAqc 415 5,43878
659JT 415 5,64902
6pimb 412 5,89894
820wg 407 5,13133
88Gxj 414 5,69620
8qUri 412 5,77401
HkNC 415 5,46975
HzZG 411 5,97482
N49S 415 5,48089
NBGr 393 5,51528
b1rm 407 5,31185
xBeY 411 6,06270
A0A8S5T203 411 5,77401
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_34085
8plKk
 11 43,8% 299 6.812E-74
2 phalp2_16214
4K5QO
 38 31,3% 303 1.180E-37
3 phalp2_36626
25FVY
 29 27,5% 280 1.453E-29
4 phalp2_11379
3RDO
 2 24,0% 362 9.977E-19
5 phalp2_13871
7vJcf
 1 31,3% 249 1.777E-18

Domains

Domains [InterPro]
Ami2
Unannotated
holin_Dp1
Representative sequence (used for alignment): 8dsHX (407 AA)
Member sequence: A0A8S5SXN8 (415 AA)
1 407 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF16938

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctAkS7
[NCBI] 		2827798 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK032698 [NCBI]
CDS location
range 8172 -> 9419
strand +
CDS
ATGAGTAATTCTAATCTAGTCACAAGGTGGTGGCCAGCTAATCCAACCAACTACACTGGGGGTCGCGAGGGTAATAAAATCCAAGGCATTGTAGTACACCACGCTGCTTCAACTTCACTCGACTCTATCGGTGCAGCATTTGCTCGTGCTGGACGTAATGGTTCTGCACATTATGGTGTAGGGGGTAACCAAATCCATCAATACGTAGATGAAGCTAACACTACCTGGCATTGTTCTAACTGGTGGGGCAATGTTAGGACGGTCGGCATTGAGACGACTAACTCCACAGGAGCTCCTAACTGGGAAATCGCACCGGATACCTTCGAGACACTCGTGAAACTCGTGGCAGATATTGCTAAGCGTAATAATCTTGGCAAACTCTGGATCAACCCTAAGGCAGATATGCCTGTTTTGTCCGGCCATAAAGACTGGTATGGAGCTTCTACCGTTTGCCCAGGACCATCACTCTACCCTAGACTTCAAGAAATTGCAGATCGCGCCAATGCGATTAACTTCCCACCAGTAGTCGAAGTAAAACCAATGATAGTCTGGGTTAATATCGAACCGCTAGTGCTAAAAGCCAAAGCAAAAGTAGATATTATTGATCTCGATACAGGCGCAGATAAAGGTGATATTCAAGCGGGAATGGTTATCAGTAATCTCGTGCAAGAGACAACTGTAAATGGCAAGAAATACTATCGTACTCTCTATTCAAAGACTAAAGGCTTCAATAATGGTATTTTAGCTGAAGCGTTAGAACCAATGGTTGCGCCAGTATCTCCAGATTCAGTTATTAACGGTGGGGTTAAGGAAACAAAACCAGAAATCAAAGTTTCTACCGAAGAAGAGAAACGACCAATCACAGAAAAAGAGGTTAAAGAAGAAGCTAAGAAAGATGACGGATTTGGCGACGATATCGCACCAGTGCCAGGGGTAAAACCTGGTATGAAGCCAGAAGAAGTTCAAAAATTAATCGAGGAGCAAAAGAAAAATATGAAAATAGCAGAAGAAATTATTGATGAGGTAAACAAAGACATGGAATTCTCACCAAAAACTAAAATGATCGTGTATTTATTGGGCGATCTACTAATTATCGCAGGAACTCAGGCGCCACTCGTGGTTGCACTCGTTAATACCACTGATGCCGTGACTTTTGGTCAATTATTAGGTCAATTATTACTATCTACAGGTACGATGTTGCTATTTACTTTTAAGTTATTGAAAAAGAAAGGTGAAAAGAATGTCTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0016020 membrane cellular component None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (8dsHX) rather than this protein.
PDB ID
8dsHX
Method AlphaFoldv2
Resolution 82.94
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50