Protein

Protein accession
A0A8S5SNF7 [UniProt]
Representative
A4V0
Source
UniProt (cluster: phalp2_38352)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MDIKRLIVSDEVSNRVSYGRGNPRNYLTIHQTGNTSAGANAMAHHRLQARSGVGYGWHWQVDDHEAIQTHDHSFKIWNAGDGRGKGNTESISIEICVNLDGDYNQAVENGAKLSAMILKEENIDISKMVQHNYWSGKNCPEQIRACKNGICWSNFVEKVKGYLNASNEPIGWIKDNVGWWYRLPNGDYAKNSWSKINGEWYYFDSEGYAYCEKWLKYKGEWYYFDKDCKMLCEKWLTVGEETFYFNDWGVCDTSYVRNIEGKEYAFNERGALIKDKIINKDGVIE
Physico‐chemical
properties
protein length:285 AA
molecular weight:32945,4 Da
isoelectric point:5,95
hydropathy:-0,72
Representative Protein Details
Accession
A4V0
Protein name
A4V0
Sequence length
291 AA
Molecular weight
33781,23260 Da
Isoelectric point
6,16325
Sequence
MKIERLIVPDSVSNRVSFGRGNPRNYLTIHQTGNTDPGSNAKAHHNLQARGGVGYGWHWQVDDEFAIQTHDHSFKIWHAGDGRGKGNTESISIEICVNSDGDYNQSVENGAKLAALILKEENIDISKMVQHNYWTGKNCPEQIRACKNGICWSHFVEKVKGYLNGSNEPIGWRYEEETGKWWYRLPNGDYAKSSWSKINGEWYYFDSEGYAYCEKWLKYKGEWYYFDKDCKMLCEKWLTIGEETFYFNDWGVCDLNYVRNIDGKQYAFNERGALIKNKIIEENGSIENLKK
Other Proteins in cluster: phalp2_38352
Total (incl. this protein): 20 Avg length: 291,0 Avg pI: 6,14
Protein ID Length (AA) pI
A4V0 291 6,16325
5RiGo 289 6,31609
63EYm 305 5,80635
6ZEQN 291 6,06253
6ZgRO 289 6,75363
6Zu51 289 5,76310
6tjBB 291 5,94765
70zEq 291 6,21861
78ZWN 305 5,74048
7NT1H 291 6,06525
HISK 291 6,21861
HkCU 291 6,08077
HtxS 289 6,31722
NMPz 285 5,88445
NsXA 289 6,37338
Oce5 285 6,36951
oDo2 290 6,12926
yeaL 291 5,95624
yg6o 291 6,75295
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_7826
aMK
 29 46,8% 190 4.474E-50
2 phalp2_24718
6w5Af
 15 32,2% 282 5.444E-44
3 phalp2_3370
3jRfu
 70 31,4% 248 5.887E-31
4 phalp2_30595
6aPsK
 7 29,3% 290 5.821E-27
5 phalp2_36615
23Gpv
 52 33,0% 248 1.073E-26
6 phalp2_10049
7q2PM
 57 28,3% 300 1.188E-23
7 phalp2_9807
8qUHY
 2 30,3% 204 2.299E-20
8 phalp2_1064
7bl2o
 2 29,7% 276 2.824E-18
9 phalp2_5248
854PA
 247 26,4% 280 6.098E-16
10 phalp2_36039
5QA7W
 56 28,8% 243 3.406E-10

Domains

Domains [InterPro]
Ami2
Choline_bind_3
Representative sequence (used for alignment): A4V0 (291 AA)
Member sequence: A0A8S5SNF7 (285 AA)
1 291 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF19127

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctnR613
[NCBI] 		2827939 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK032640 [NCBI]
CDS location
range 9251 -> 10108
strand -
CDS
ATGGATATTAAAAGATTAATTGTATCAGATGAAGTTTCAAATAGAGTTAGTTATGGCAGAGGCAATCCAAGAAACTATTTAACAATTCATCAAACAGGAAATACATCAGCAGGAGCGAACGCTATGGCACATCATAGACTACAAGCTAGAAGTGGTGTAGGCTATGGTTGGCATTGGCAAGTTGACGACCACGAAGCTATACAAACACACGACCATTCATTCAAAATATGGAACGCAGGAGATGGCAGAGGAAAAGGAAATACTGAAAGTATCTCAATAGAAATTTGTGTCAATTTAGATGGCGACTATAACCAAGCTGTAGAAAACGGAGCAAAATTATCAGCAATGATACTTAAAGAAGAAAATATCGACATTTCAAAAATGGTTCAACATAACTATTGGAGTGGAAAGAATTGTCCTGAACAGATAAGGGCTTGTAAAAATGGTATTTGTTGGAGTAATTTTGTAGAAAAGGTTAAAGGTTACCTAAATGCAAGCAACGAGCCTATTGGTTGGATAAAAGATAATGTTGGTTGGTGGTATAGACTACCAAACGGAGATTATGCGAAAAATAGCTGGTCTAAAATCAATGGCGAATGGTATTACTTTGATAGTGAGGGCTATGCTTATTGTGAAAAATGGTTGAAATATAAAGGCGAATGGTACTATTTTGATAAAGATTGTAAAATGCTTTGTGAAAAGTGGTTGACTGTTGGCGAAGAAACATTTTATTTCAACGATTGGGGTGTTTGTGATACAAGTTATGTTAGAAATATTGAGGGCAAAGAATATGCTTTTAATGAAAGAGGAGCATTAATAAAAGATAAAATAATAAACAAAGACGGAGTTATAGAGTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030420 establishment of competence for transformation biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (A4V0) rather than this protein.
PDB ID
A4V0
Method AlphaFoldv2
Resolution 90.76
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50