Protein

Protein accession
A0A8S5RT46 [UniProt]
Representative
4uNwu
Source
UniProt (cluster: phalp2_34425)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MKLVKDNQTANFFANKVNTCEYIVLHHTGKGQGINIVNYLCRKESGVSAHYVVDIDGTVYQLAEDKKCTRHAGVSAYQGKTGLNLYSIGIEVVSDGYSFTDAQREATRELVRSLMQAHNIPSSKIIRHKDIAPGRKRDIGDNFRNNQFSTFAEYQKSFDQKEAKSLTPEAEEARKLGIWNGLDPDKPATREQVAMMIYRAAKLNN
Physico‐chemical
properties
protein length:205 AA
molecular weight:23060,8 Da
isoelectric point:9,02
hydropathy:-0,61
Representative Protein Details
Accession
4uNwu
Protein name
4uNwu
Sequence length
206 AA
Molecular weight
24797,94730 Da
Isoelectric point
8,98523
Sequence
MKIIHDYKTNKISRGLNSWSYIMLHHTWPWADLNLVKYLAWSSAPVSCHFVVWKKGDIWQLSELGARTWHAWVSKRQWLTELNQHAIGIEICSNWTDFNDIQRQAVRELTNYLLSMLNLTPDKIITHADVAGFRGKRDVWPNFWNNKHASWEEYQNSYKKEPLTEKERNNLELLKRLNSWFWNKFEFTELRNDLHKTNKTIDLILE
Other Proteins in cluster: phalp2_34425
Total (incl. this protein): 4 Avg length: 210,0 Avg pI: 8,73
Protein ID Length (AA) pI
4uNwu 206 8,98523
3bwk6 206 8,98523
80bcY 223 7,92118
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_4742
7FuH3
 9 36,1% 166 3.727E-43
2 phalp2_21805
4f0s7
 9 36,4% 159 4.592E-42
3 phalp2_1605
81mMl
 270 28,7% 198 1.186E-27
4 phalp2_31152
3LHkO
 133 23,3% 180 2.651E-21
5 phalp2_21916
4KsAl
 20 27,2% 147 6.720E-21
6 phalp2_24299
3nIle
 36 28,3% 155 2.398E-18
7 phalp2_34832
3TNM2
 9 25,3% 205 8.241E-18
8 phalp2_29669
8sfID
 1005 26,6% 165 5.238E-17
9 phalp2_12341
2FfN
 1 28,1% 149 9.696E-17
10 phalp2_22919
33qi6
 180 23,6% 173 4.515E-16

Domains

Domains [InterPro]
Ami2
Representative sequence (used for alignment): 4uNwu (206 AA)
Member sequence: A0A8S5RT46 (205 AA)
1 206 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Caudovirales sp. gcode 4
[NCBI] 		2838363 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK059153 [NCBI]
CDS location
range 26641 -> 27258
strand +
CDS
ATGAAATTGGTTAAAGATAATCAGACCGCGAACTTCTTCGCAAATAAGGTCAATACCTGCGAGTATATTGTCCTCCATCATACAGGGAAAGGACAAGGAATCAATATTGTCAACTACCTCTGCAGAAAGGAAAGCGGAGTCTCAGCTCACTATGTTGTAGATATTGATGGAACAGTCTACCAACTCGCAGAGGATAAAAAATGTACTCGGCACGCTGGGGTGTCTGCATATCAAGGGAAGACTGGACTCAATCTCTACTCGATAGGAATTGAGGTTGTGAGTGATGGTTACAGCTTTACTGATGCTCAGAGAGAAGCCACAAGAGAACTTGTAAGAAGTCTGATGCAAGCTCACAATATCCCTAGTTCTAAGATCATCAGACACAAAGACATCGCACCAGGAAGAAAGCGAGATATAGGGGACAACTTCCGAAATAATCAGTTTTCAACTTTTGCTGAATATCAGAAAAGCTTTGATCAAAAAGAGGCTAAAAGTCTGACTCCAGAGGCTGAAGAAGCTCGTAAGCTCGGAATATGGAATGGATTAGATCCAGACAAGCCAGCAACAAGAGAGCAAGTAGCGATGATGATATACAGAGCCGCAAAGCTCAATAATTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (4uNwu) rather than this protein.
PDB ID
4uNwu
Method AlphaFoldv2
Resolution 84.15
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50