Protein

Protein accession
A0A8S5R3R4 [UniProt]
Representative
3tNNt
Source
UniProt (cluster: phalp2_40045)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MRISVKLTRAENTALLGAGPVELDLEEYLCGVVPSEIYESSHMEALKAQAVAARTFAVKRAMAGTVVDDTTSFQAYRATLAKSSPRSRQAVEETAGQVLTYGGEVIDCFYSASNGGTCKRSGEVWSRDYSYYVNRPDPWDTAARAEKPTNSSHGVGLSQVGCMWAAKQGVPYNEILAFYYNGAALVHEYGTGSVVGFEEETGGFAMNLHKLIFTNNACYKAGRKITPKGIMVHSTGANNPWLKRYVGPDDGLLGKNQYNNHWNQSMDREVCVHAFIGKLADGTIATYQTLPWDYRGWHCGSGSKGSGNNTHISFEICEDGLTDSSYFNKVYNEAVELCAYLCKQYGLTEKNIICHSEGHAQGIASNHGDVMHWFPKHGKNMDTFRAAVKAKLAGSSSGNTGGSAGSGTLYRVRKSWSDASSQLGAFAILGNAKELADKNPGYAVFDEAGKKVYPSASGSTGSGTLYRVRKSWADAASQKGAFNVLDNAKRCADENPGYSVFDEAGKVVYAGSAAASTYTVQKGDSLWAIAEKRLGNGTRYNEIKKLNGLTSDVIYAGQVLKLPNR
Physico‐chemical
properties
protein length:565 AA
molecular weight:61058,5 Da
isoelectric point:8,41
hydropathy:-0,40
Representative Protein Details
Accession
3tNNt
Protein name
3tNNt
Sequence length
565 AA
Molecular weight
N/A Da
Isoelectric point
8,10995
Sequence
MKIFVKLTRAENTALLGAGPVELDLEEYLCGVVPSEIYESSHMEALKAQAVAARTFAVKRAMAGTVXXXXTSFQAYRAPLAESSPRSRQAVVETAGQVLTYGGEVIDCFYSASNGGTCKRSGEVWSRDYPYYVNKPDPWDTAARTEKSTNPSHGVGLSQVGCMWAAKQGVPYNEILAFYYSGAALVHEYGTGSVVGFEEETGGFAMNLHTLIFTNNACYKAGRKITPKGIMVHSTGANNPWLKRYVGPDDGLLGKNQYNNHWNQPMDREVCVHAFIGKLADGTIATYQTLPWDHRGWHCGSGSKGSGNNTHISFEICEDGLTDSAYFNKVYNEAVELCAYLCKQYGLTEKNIICHSEGHAQGIASNHGDVMHWFPKHGKNMDTFRAAVKAKLTGSSSGNTGGSAGSGTLYRVRKSWSDASSQLGAFAVLDNAKELADKNPGYAVFDEAGKKVYPSASGSTGSSTLYRVRKSWADAASQKGAFNVLDNAKRCADENPGYSVFDEAGKVVYAGSAAASTYTVQKGDSLWAIAEKHLGNGTRYNEIKKLNGLTSDVIYAGQVLKLPNR
Other Proteins in cluster: phalp2_40045
Total (incl. this protein): 34 Avg length: 553,5 Avg pI: 7,65
Protein ID Length (AA) pI
3tNNt 565 8,10995
10tcT 551 8,41675
10tiI 555 8,11833
14IR8 558 7,54330
1dagk 523 6,50968
1djAz 537 6,60386
1mYWj 566 6,88754
25mfo 550 8,32398
2Tvt8 604 7,58422
2UmpV 602 7,58422
2lX72 565 6,37474
3d0g6 553 8,52538
3lS2b 565 7,55273
3uKGX 565 7,87476
3vszb 565 7,56035
4ud0t 548 6,76437
4z6ns 442 6,67173
5uclV 602 7,58422
6Q9w4 554 8,74438
6UMC2 552 6,73647
6WXGA 550 8,15017
6Xpee 551 8,10576
76N7Q 510 8,83702
7nIg1 565 6,88806
7rTcA 565 7,55148
7u9q0 517 8,44892
BR4n 565 7,22397
CWmi 565 7,16554
ZBPq 602 7,58422
ZIJN 599 8,54730
ZJww 602 7,58422
naYS 510 8,69100
oeZG 431 6,67400
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_3379
3pnB5
 146 45,8% 388 1.485E-96
2 phalp2_18959
4116S
 8 41,1% 418 7.283E-77

Domains

Domains [InterPro]
SpoIID
Ami2
Unannotated
Unannotated
LysM
Representative sequence (used for alignment): 3tNNt (565 AA)
Member sequence: A0A8S5R3R4 (565 AA)
1 565 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01476, PF01510, PF08486

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctsAY3
[NCBI] 		2827281 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015802 [NCBI]
CDS location
range 39108 -> 40805
strand +
CDS
ATGAGGATCTCCGTAAAACTGACCCGCGCCGAGAATACCGCCCTCCTTGGCGCGGGGCCGGTAGAGCTGGATTTGGAGGAATACCTCTGCGGCGTGGTGCCTTCTGAGATTTATGAAAGCTCCCACATGGAGGCGCTTAAAGCGCAGGCAGTGGCCGCCCGCACCTTTGCGGTCAAGCGGGCCATGGCGGGGACGGTGGTAGACGACACCACCAGCTTTCAGGCATACCGCGCCACTCTGGCAAAGTCCAGCCCCCGCAGCAGGCAGGCCGTGGAGGAAACCGCCGGGCAGGTGCTTACCTATGGCGGCGAAGTCATTGACTGCTTTTATTCGGCTTCAAACGGCGGCACCTGCAAACGCAGTGGAGAGGTTTGGAGCCGGGACTATTCTTACTATGTGAACAGGCCTGATCCGTGGGACACGGCGGCCCGCGCAGAGAAACCCACTAATTCCAGCCATGGCGTGGGGCTTTCCCAAGTGGGCTGTATGTGGGCCGCCAAGCAGGGTGTCCCTTACAACGAGATACTGGCCTTTTATTACAACGGCGCTGCTTTGGTGCATGAATACGGCACCGGCAGCGTCGTTGGTTTTGAAGAAGAAACAGGAGGTTTCGCTATGAATCTGCATAAACTGATTTTCACGAACAACGCCTGCTACAAGGCAGGCCGCAAGATTACCCCCAAGGGCATTATGGTGCATAGCACCGGGGCCAACAACCCGTGGCTGAAACGCTATGTCGGCCCGGATGATGGCCTGCTGGGAAAGAACCAGTACAACAATCACTGGAACCAGTCCATGGATCGGGAGGTCTGCGTTCACGCTTTTATCGGCAAGCTGGCGGATGGGACGATTGCCACTTACCAGACGCTTCCTTGGGATTACCGGGGCTGGCATTGCGGCTCCGGTTCCAAGGGCTCCGGCAATAATACCCACATCTCTTTTGAAATTTGCGAGGATGGCCTGACAGACTCCTCCTATTTCAACAAGGTCTACAATGAGGCGGTGGAGCTTTGCGCTTACCTCTGCAAGCAGTACGGCCTGACCGAGAAAAATATCATCTGCCACAGCGAGGGCCATGCGCAGGGCATTGCCTCCAATCACGGGGATGTCATGCACTGGTTCCCGAAGCATGGGAAGAACATGGACACATTCCGCGCAGCGGTGAAGGCCAAGCTGGCTGGTTCTTCTTCCGGCAATACCGGAGGTTCTGCGGGCAGCGGCACCCTCTACCGGGTGAGAAAGTCTTGGAGCGATGCCTCCTCCCAGCTCGGAGCCTTTGCGATTTTGGGTAATGCCAAAGAGCTGGCGGACAAGAATCCCGGCTATGCGGTCTTTGACGAAGCTGGGAAGAAGGTTTATCCCTCTGCCTCTGGAAGCACCGGCAGCGGCACCCTTTACCGGGTGCGCAAGAGCTGGGCGGATGCCGCTTCTCAAAAGGGCGCTTTCAATGTGCTGGACAATGCGAAACGCTGTGCCGACGAAAATCCCGGTTACTCTGTTTTTGACGAGGCGGGAAAGGTTGTATATGCGGGCAGCGCAGCCGCTTCTACTTACACGGTGCAGAAGGGCGACAGCTTGTGGGCCATTGCAGAAAAGCGCCTCGGAAACGGCACCCGGTACAATGAGATCAAGAAACTGAACGGCCTGACCTCCGACGTAATTTATGCGGGGCAGGTCTTGAAACTTCCCAACAGGTAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0030435 sporulation resulting in formation of a cellular spore biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (3tNNt) rather than this protein.
PDB ID
3tNNt
Method AlphaFoldv2
Resolution 85.61
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50