Protein

Protein accession
A0A8S5QL56 [UniProt]
Representative
3sm29
Source
UniProt (cluster: phalp2_32904)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MGNLYAESGLNPINLQNTYEKRLGLTDAEYTAAVDSGSYSNFVRDSAGYGLAQWTYWSRKEAMLNYARKTGASIGDLMMQLDFMFQELKGHVAVFQVLRTARTVKEASDIVLTKYERPADMSNAVKVKRASFGQAYYDAYANTTTTPGKEEITVSNSPLVTYTNITKNKTSPRNHAIDTITIHCIVGQWTAKQGCDFFATTDRECSANYIVGKDGSIGLSVDEADRSWCTSSRENDNRAITIEVASDTEHPYAVTDAAYAALIKLIADICKRNGIKKLIWSTNKADRVNHANGCNMTVHRDYANKACPGQYLYDRHGAIAAAVNELLGSGTTQPPEAAPGAVQGFPATPFTVRVIIPDLNYRKGPGMGYAVRGQTGKGVFTITEVQDGWGKLKSGAGWIYLENPDYCTIQGVAAKPAEPDPADVLAQEIAGKVKGSGLDPADVLNRVEKILGVA
Physico‐chemical
properties
protein length:454 AA
molecular weight:49303,9 Da
isoelectric point:6,67
hydropathy:-0,33
Representative Protein Details
Accession
3sm29
Protein name
3sm29
Sequence length
432 AA
Molecular weight
N/A Da
Isoelectric point
9,15665
Sequence
MFNSIVENLVSIGWSMLIFLSAYLANVTFSLWYNIKLLHEPFDREKLINSAYKIATFVIGLTLLCVALTTLPLFANEVGWAIPQEYTDLFADLVIIGAVLLVSCKYIKEAFVKFTEILNAGTSDAQGKXXXXTMSNSPLVDYTRISPNKNSPRNHKIDTITIHCVVGQCTVETLGNIFAPTSRQASSNYGVGTDGKIGMYVEEKDRSWCSSNAANDNRAVTIEVASDTKHPYAVNDRAFAALLDLVTDICKRNGIKKLVWSTKKADRVNHKNGCNMTVHRDYANKSCPGDYLYNRHGEIAAEVNRRLGVADTAPDAGAAQGVTVYTVKKGDTLSQIAAKYGTTYQAIAAYNGIKNPNAIRVGQKIKIPASTAPAALKKGDRVKVLNAVTYDGKPFRTYYDTYDVIQVSGARVVIGVGATVTAAVNAANLRKV
Other Proteins in cluster: phalp2_32904
Total (incl. this protein): 6 Avg length: 359,5 Avg pI: 8,79
Protein ID Length (AA) pI
3sm29 432 9,15665
H7BW58 334 9,21526
A0A8S5RMA6 329 9,35818
D6QWN4 302 8,80962
H7BWC9 306 9,54836
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_1650
8m87G
 265 48,5% 284 1.179E-78
2 phalp2_15597
2fZru
 53 41,0% 341 6.846E-70
3 phalp2_27209
3o7E3
 7 37,9% 343 7.137E-68
4 phalp2_453
84Dz
 2 27,0% 310 8.685E-25

Domains

Domains [InterPro]
Unannotated
Ami2
LysM
Unannotated
Representative sequence (used for alignment): 3sm29 (432 AA)
Member sequence: A0A8S5QL56 (454 AA)
1 432 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01476, PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ct0hG5
[NCBI] 		2826269 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015677 [NCBI]
CDS location
range 1954 -> 3318
strand -
CDS
ATGGGCAACCTCTACGCCGAGAGCGGGCTCAATCCGATAAACCTCCAGAACACCTACGAGAAGCGCCTGGGCCTCACGGACGCCGAGTACACGGCCGCCGTGGACTCCGGGAGCTACTCCAACTTCGTCCGCGACAGCGCCGGCTACGGCCTCGCGCAGTGGACATACTGGAGCCGCAAGGAGGCCATGCTCAACTATGCCCGGAAGACCGGCGCGTCCATCGGCGACCTGATGATGCAGCTCGACTTCATGTTCCAGGAGCTGAAGGGCCACGTGGCCGTCTTCCAGGTACTCCGGACAGCCCGGACCGTGAAGGAGGCGTCCGACATCGTGCTGACCAAGTACGAGCGCCCAGCCGACATGAGCAACGCCGTCAAGGTAAAGCGGGCCAGCTTCGGCCAGGCATACTACGACGCCTACGCAAACACTACAACAACCCCAGGGAAGGAGGAGATCACCGTGAGCAACAGCCCTCTGGTAACGTACACCAACATCACCAAGAACAAGACCAGCCCCCGCAACCACGCCATCGACACCATCACGATCCACTGCATCGTGGGCCAGTGGACGGCGAAGCAGGGCTGCGATTTTTTTGCCACCACTGACCGCGAGTGCAGCGCCAACTACATCGTCGGCAAGGATGGCTCCATCGGCCTGTCCGTCGATGAGGCGGATCGCTCCTGGTGCACTTCCAGCCGTGAGAACGACAACCGCGCCATCACAATCGAAGTCGCCAGCGACACCGAGCACCCCTACGCCGTGACCGACGCAGCCTACGCCGCACTGATCAAGCTGATAGCCGACATTTGCAAGCGCAACGGCATCAAGAAGCTGATCTGGTCCACCAACAAGGCCGACCGCGTCAACCACGCCAACGGCTGCAACATGACCGTGCACCGCGACTATGCCAACAAGGCCTGCCCGGGCCAGTACCTCTACGACCGCCACGGCGCCATCGCTGCGGCCGTCAATGAGCTCCTGGGCTCTGGCACTACCCAGCCACCCGAGGCGGCTCCGGGGGCCGTCCAGGGCTTCCCTGCGACGCCCTTCACTGTCCGCGTCATTATCCCGGATCTGAACTACCGCAAGGGCCCCGGCATGGGCTACGCAGTCAGAGGCCAGACCGGCAAGGGCGTCTTTACCATCACCGAGGTGCAGGACGGCTGGGGCAAGCTGAAAAGCGGCGCCGGCTGGATCTACCTCGAAAACCCTGACTACTGCACCATCCAGGGCGTCGCAGCGAAGCCGGCCGAGCCGGATCCTGCTGACGTGCTGGCGCAGGAGATCGCCGGCAAGGTGAAGGGCTCCGGCCTGGATCCTGCTGACGTTCTGAACAGGGTCGAGAAGATCCTGGGCGTGGCATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (3sm29) rather than this protein.
PDB ID
3sm29
Method AlphaFoldv2
Resolution 84.63
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50