Protein

Protein accession
A0A8S5QKV4 [UniProt]
Representative
366VA
Source
UniProt (cluster: phalp2_13250)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MEFVSCDPSNYRVGRTQPVRYIVLHYTAGNGDTARNNCDYYHRVGGLQASAHYFVDEHGAMQSVREGDTAWHCGARAYWHPECRNGNSIGIEMCSRKRADGSYYILPETVANAAALAREIMQRYGIDTDHVLRHYDVTGKRCPMPWVDDPAQWTAFLAMLTPEHPNEEEEGPMIRYNKIDDIPAWARSDVQRLIDRGALRGNGRGELDLSPDMLRTLIVCQRMVDQAKET
Physico‐chemical
properties
protein length:230 AA
molecular weight:26164,1 Da
isoelectric point:5,84
hydropathy:-0,61
Representative Protein Details
Accession
366VA
Protein name
366VA
Sequence length
426 AA
Molecular weight
47683,54250 Da
Isoelectric point
8,62434
Sequence
MQFNPARPENYSPGRTKKIEYIVIHYTGNDNDTARSNCHYFANYATQTSAHYFVDDRETLQSVEDTDTAYHCGATNYLHQWCRNANSIGIEMCSKKDADGNFCISEDTKAGTIALARALMKQYNIPPANVLRHYDVTGKLCPEPFVRELWQWEDFKFRLNNGRDAMQGQSIIIKPEEIIVSGLYRNINRKSMSTIVNESGAKVCINAGFYTLSTFTPCASGLKIDGVKYVDGFDPGYSFDRSSVIFNDKNSIGYPDYIGGYPALIKASKAFKSNVSKEPHWDIANARSAMGLLDNGNIYLFCTGKPGITMDGLRNAMLSAGCKDAINLDGGASSQCYFDGRLIKGERDVYSYILIWRKKDLIKKGHRGRCVQYMQRKLLLRGYQLPIFGSDGDFGNETYAALTAFQKAKGLTVDGVCGPKTWEKLI
Other Proteins in cluster: phalp2_13250
Total (incl. this protein): 3 Avg length: 287,7 Avg pI: 7,17
Protein ID Length (AA) pI
366VA 426 8,62434
A0A8S5NRN7 207 7,04402
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_25199
21D2a
 10 28,5% 470 3.516E-39
2 phalp2_7198
3ejcO
 5 27,9% 426 3.986E-35
3 phalp2_17898
EGF4
 130 26,6% 420 3.850E-28
4 phalp2_24835
7sl6i
 39 25,1% 413 2.089E-17
5 phalp2_19744
4Svbf
 2 27,5% 320 2.282E-11

Domains

Domains [InterPro]
Ami2
PG_1
Representative sequence (used for alignment): 366VA (426 AA)
Member sequence: A0A8S5QKV4 (230 AA)
1 426 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF09992|PF01471

Taxonomy

  Name Taxonomy ID Lineage
Phage Podoviridae sp. ctdRZ1
[NCBI] 		2826568 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015680 [NCBI]
CDS location
range 5566 -> 6258
strand -
CDS
ATGGAATTTGTTTCTTGCGATCCGTCAAATTACCGCGTCGGGCGCACGCAGCCGGTGCGGTACATTGTGCTGCACTACACGGCCGGCAACGGCGACACGGCGCGCAACAACTGCGACTACTACCACCGCGTGGGCGGCCTGCAGGCCAGCGCGCACTATTTCGTGGACGAGCACGGCGCGATGCAGTCCGTGCGCGAGGGCGACACGGCGTGGCACTGCGGCGCGCGGGCGTACTGGCATCCCGAGTGCCGCAACGGCAACAGCATCGGCATTGAGATGTGCAGCCGCAAGCGCGCCGACGGCAGCTACTACATCCTGCCGGAGACCGTGGCCAACGCCGCGGCGCTGGCGCGGGAGATCATGCAGCGCTATGGCATCGACACCGACCACGTGCTGCGGCACTACGACGTGACGGGCAAGCGCTGCCCCATGCCGTGGGTGGATGACCCGGCGCAGTGGACGGCATTTCTGGCCATGCTGACGCCGGAACACCCGAACGAAGAGGAGGAAGGACCCATGATACGATACAACAAAATCGACGACATACCCGCGTGGGCGCGCAGCGATGTGCAGCGGCTCATCGACCGCGGCGCACTGCGGGGCAATGGACGCGGCGAGCTGGATCTCTCGCCGGACATGCTGCGCACGCTGATCGTGTGCCAGCGGATGGTCGATCAGGCAAAGGAGACATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (366VA) rather than this protein.
PDB ID
366VA
Method AlphaFoldv2
Resolution 91.93
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50