Protein

Protein accession
A0A8S5QFP0 [UniProt]
Representative
68i8d
Source
UniProt (cluster: phalp2_242)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MQREEWAAEENEPEGDLRAEPCAECRAQEENTFERMIFMSEATIWSFFKKKGLSDCGVAGLMGNLYAESGLKSTNLQNTYEKKLGMSDTDYTAQIDAGIYQDFVHDSAGYGLAQWTFWSRKQNLLAFALSRGKSIGDLEMQLDFLWKELTESYTSLVSILKTATSVRAASDAVLVQFERPADQSETAKARRASYGQKYYDQYAGKEGVFAMSNSPLVSYTKLSPNHSGKRNHVIDTISIHCMAGDLTVETCGNLFANPSRKASSNYGIGSDGRIGLYVEECNRSWCTSSSSNDNRAITIEVANNGGADQGWPVSDAAYNSLIALLVDICQRNGIKKLLWKGDKSLIGQVDKQNMTVHRWFAAKACPGDWLYNRHGQIAAEVNAKLSGEDDDMDQTKFNEMFGTAMTNYLKGLQNNNCGTWSEEARVWAENVGLFAGNGTTVDGKPNMMWPSGLTREQAAQLFYRFAQMVGLA
Physico‐chemical
properties
protein length:472 AA
molecular weight:52338,0 Da
isoelectric point:5,24
hydropathy:-0,46
Representative Protein Details
Accession
68i8d
Protein name
68i8d
Sequence length
450 AA
Molecular weight
50045,60960 Da
Isoelectric point
7,98739
Sequence
MIGMSEVFIWNFLKKKGLSDWGTAGLMGNLYAESGLKPTNLQNTYEKKLGLSDEDYTAQVDSGIYHDFVHDCAGYGLAQWTFWSRKQNLLAFARNKGTSIGDLEMQMNFLWKELTTGYPALVEVLMSATTVQAASDAVLTQFERPADQSKRAKAKRASYGQKYYDQYAGKGETMPSTNIVPAVKRLLETARAEIGYVEKETNAQLDNKTANAGDNNWNKYARDLDAIGIVYNGRKNGYAWCDIFTDWCFIRTFGLEQGMSLLCQAKSGLGAGCTYSANYYKQKGQFHTRNPQPGDQIFFTKDGGKTMYHTGIVEKVSGGRVYTIEGNTSSQPGVVPNGGCVRGKNYPLNASYIGGYGRPDFSIVQEDDDMDQNKFNEMFAVAMTNYLKSQQTNKCGDWSKDARNWTQSTGLFAGNGTTVNGEPNMMWPSGLTREQAAQLFYRFAQMVGLA
Other Proteins in cluster: phalp2_242
Total (incl. this protein): 67 Avg length: 467,6 Avg pI: 6,09
Protein ID Length (AA) pI
68i8d 450 7,98739
1gCya 456 4,66974
1kbNU 473 6,20712
1kveE 548 5,38307
1lXyj 361 8,63169
1mbLy 434 6,62040
1nSMm 456 5,27843
21Fof 534 5,26593
21H04 526 5,52557
239Q4 540 5,49357
23RCb 527 5,37188
23cu9 477 5,08541
23tPc 526 5,77174
24J9C 468 5,97715
24kHR 526 5,67767
24sjm 540 5,44207
2bNra 439 4,99123
2mCiY 433 6,67349
38Pp0 538 5,16953
3VDJs 528 5,55962
3VGOu 469 4,89085
3WBZ3 477 4,99555
3WM2L 469 4,80309
3ZB9Z 543 5,84938
3dYVb 540 5,21887
3ksfg 378 9,04448
3uCHZ 433 6,47359
3uz1O 448 6,19945
3w4oF 448 6,06309
3weml 379 9,04429
40eed 501 4,71982
415bH 488 5,70285
41qve 534 5,50084
4L0s7 529 5,65232
4VuHC 437 6,60739
4k73u 532 5,20403
4ypHF 471 9,11307
4z20c 526 9,00760
5LoOO 456 5,06375
5QzVk 451 5,97102
5RUTU 437 6,12562
5RZf2 437 6,12562
5SnqZ 448 5,27508
5VR9H 456 5,12446
5XHVm 451 6,06025
62dtq 437 6,61074
63eMH 451 6,14278
64RIc 456 5,26877
69lh1 450 6,90062
6Zvue 436 6,04212
6ble6 429 5,30236
6bte0 450 8,01672
6d8MN 450 8,45369
6j6RS 483 5,49510
6juzY 487 8,96634
6nOJk 460 5,38705
6pJfS 347 5,73360
6rC0w 444 5,28361
7Mfzm 433 6,41737
7vCiZ 433 6,41641
81fvI 434 5,63066
88AaF 448 5,87632
8cxt1 448 6,55862
8otMK 433 6,67423
8rXuu 480 5,23938
gpBB 450 5,79004
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_18242
4UeaR
 8 61,6% 313 9.676E-134
2 phalp2_22696
7Vmwp
 2 42,4% 365 5.271E-117
3 phalp2_8960
3yCnS
 526 45,5% 378 5.561E-101
4 phalp2_22655
23BKV
 6 39,1% 304 1.992E-75
5 phalp2_37104
1djTo
 247 37,4% 393 6.887E-75
6 phalp2_21233
1gBO4
 3 35,3% 371 6.948E-66
7 phalp2_30431
7DEWH
 4 28,8% 343 1.745E-49
8 phalp2_5524
3rdGp
 29 27,1% 369 1.652E-33

Domains

Domains [InterPro]
Phage_lys2
CHAP
Unannotated
Representative sequence (used for alignment): 68i8d (450 AA)
Member sequence: A0A8S5QFP0 (472 AA)
1 450 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF05257, PF18013

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctEBu1
[NCBI] 		2825393 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015651 [NCBI]
CDS location
range 35546 -> 36964
strand -
CDS
ATGCAACGCGAAGAATGGGCCGCAGAAGAAAATGAGCCAGAAGGAGATCTTCGCGCAGAACCGTGCGCTGAATGCCGCGCGCAGGAAGAGAACACATTCGAGAGGATGATATTCATGTCGGAAGCAACCATTTGGAGTTTCTTCAAGAAGAAAGGCCTCTCGGACTGCGGAGTGGCCGGCCTGATGGGGAACCTCTACGCCGAGAGCGGTTTGAAGTCAACCAACCTCCAGAACACCTACGAAAAGAAACTTGGAATGTCTGATACGGATTACACCGCTCAGATTGACGCCGGAATCTATCAGGATTTTGTTCACGACAGCGCCGGCTACGGCCTTGCTCAGTGGACGTTCTGGAGCAGAAAGCAGAATCTTCTCGCTTTTGCTCTGAGCAGAGGAAAGAGCATTGGCGATTTGGAGATGCAGTTGGATTTTCTGTGGAAGGAACTGACTGAGAGTTATACCTCTCTGGTCAGCATCTTGAAGACCGCAACATCTGTCCGGGCAGCCTCCGATGCGGTGCTGGTGCAGTTTGAACGCCCCGCAGATCAGAGCGAAACGGCTAAGGCCAGGCGAGCCTCTTACGGACAGAAATATTATGACCAATATGCCGGAAAGGAGGGCGTATTTGCAATGAGCAACAGTCCATTGGTTTCCTACACAAAGCTGTCGCCCAATCATTCCGGAAAGCGGAATCATGTGATCGACACGATTTCGATCCACTGTATGGCGGGGGACCTGACTGTGGAAACCTGCGGCAATCTTTTCGCCAATCCGAGTCGGAAAGCGAGCAGTAACTATGGAATCGGAAGCGATGGCAGGATCGGTCTCTATGTGGAGGAGTGCAACCGTTCCTGGTGCACCTCTTCCAGTTCTAATGATAACCGCGCCATCACGATTGAGGTGGCAAACAACGGCGGAGCCGACCAGGGGTGGCCGGTATCTGATGCGGCCTACAATTCTTTGATTGCCCTTCTTGTGGACATCTGTCAGAGAAATGGCATCAAGAAACTGCTTTGGAAAGGTGATAAGTCGCTGATCGGTCAGGTGGACAAGCAGAACATGACTGTTCATCGCTGGTTTGCAGCAAAGGCTTGCCCCGGAGACTGGCTTTACAACCGGCACGGGCAGATCGCCGCCGAGGTCAACGCAAAACTATCTGGGGAGGACGACGATATGGATCAAACGAAATTTAATGAGATGTTTGGTACAGCCATGACAAATTATCTCAAGGGTCTTCAGAACAATAACTGCGGCACCTGGTCTGAAGAGGCTCGGGTGTGGGCTGAAAATGTGGGGCTCTTTGCCGGAAATGGCACCACGGTTGACGGAAAGCCGAATATGATGTGGCCGTCCGGCCTGACAAGAGAGCAGGCGGCCCAGCTTTTCTATCGGTTTGCGCAGATGGTGGGGCTTGCATGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (68i8d) rather than this protein.
PDB ID
68i8d
Method AlphaFoldv2
Resolution 90.70
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50