Protein

Protein accession
A0A8S5Q394 [UniProt]
Representative
5KvkK
Source
UniProt (cluster: phalp2_5899)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MNINTSLISNNNSYAGQTPLYIVIHNTDNIAKTADAKAHATAQHNGNFHGYSAHVFVDDKSAYQALPYNRGAWHVGVNYGGKLFGIVNNRNSIGIEMCMNAGYNYEKAFQNTVDVCKQLMKKYGIPASRVVRHYDVCAKNCPSVIRGKGDWNRFKKLISSETVTVPTTKPTVKVDKYYRVRKTWKDSKSQIGAYKSLENAKKACKAGYSVFDWNGKAVYSVTAKKSVAKVAKEVINGEWGNGQDRKDRLEAAGYNYTEVQNAVNKLLK
Physico‐chemical
properties
protein length:268 AA
molecular weight:29880,5 Da
isoelectric point:9,66
hydropathy:-0,58
Representative Protein Details
Accession
5KvkK
Protein name
5KvkK
Sequence length
314 AA
Molecular weight
35460,00570 Da
Isoelectric point
9,67917
Sequence
VATTAMIPRNLIMVGILYKEFMLKSTPNGVLFIMHFFNLNNERRTYMNINTSLISNNNSYAGQTPRYIVIHNTDNIAKTADAKAHATAQHNGNFHGYSAHVFVDDKSAYQALPYNRGAWHVGVDYGGKLFGTVNNHNSIGIEMCMNAGYNYEKAYQNTVDVCKQLMKKYNIPAFRVVQHYDVCAKNCPSVIRKNGDWDRFKKLISSETVTAPTTKPTVKVDKYYRVRKTWKDSKSQIGAYKSLKNAKKACKAGYSVFDWNGKAVYSVTAKKSVAKVAKEVINGEWGNGQDRRDRLESAGYNYTEVQNAVNKLLK
Other Proteins in cluster: phalp2_5899
Total (incl. this protein): 133 Avg length: 277,2 Avg pI: 9,61
Protein ID Length (AA) pI
5KvkK 314 9,67917
1oc0K 268 9,72862
2lYNt 268 9,58260
3AsMZ 268 9,70367
3BT4f 268 9,60065
3GPLa 268 9,64391
3IUBd 268 9,53953
3l32f 308 9,72539
3lRFj 268 9,64030
3lThr 268 9,65248
3ligO 268 9,71682
3mutX 268 9,61257
3oRaE 268 9,62437
3oaHB 308 9,61335
3rPCR 308 9,67943
3sJ56 268 9,53979
3tMna 287 9,11262
3tabE 268 9,67717
3uBmg 268 9,57125
3uJhf 268 9,62418
3xwgT 343 9,56764
3yz04 268 9,57106
5KLr7 268 9,64010
5KhQr 293 9,74145
5KwHq 268 9,62437
5Lu7x 268 9,55984
5Lu8i 268 9,60690
5M3G5 268 9,57106
5M6jB 293 9,66608
5MGLQ 293 9,69110
5MIvz 268 9,65229
5NlMM 268 9,60065
5NoVK 268 9,61238
5OncH 268 9,65209
5PWVT 268 9,58260
5PZlt 268 9,66447
5QIQ5 268 9,51723
5R0W0 302 9,74893
5R9O2 293 9,73893
5RNJ4 268 9,57125
5RZI6 308 9,69116
5RaNP 293 9,66628
5S7YI 268 9,58260
5SdZE 308 9,70296
5TIGn 261 9,65261
5TMcz 268 9,63179
5U806 302 9,73681
5UVfL 268 9,62863
5V4Lj 268 9,58247
5VHtn 268 9,64030
5VhFB 268 9,49466
5W1W9 268 9,58260
5WDAK 268 9,53953
5WVIw 268 9,55114
5Wdor 268 9,60065
5X34r 302 9,67904
5Xrgl 308 9,69097
5XtiV 268 9,60065
5Y5BO 268 9,53953
5Ys3Z 268 9,49498
5Z2gf 268 9,65209
5ZCGj 268 9,53966
60lMg 268 9,61225
61mrN 268 9,58949
63bMI 268 9,65209
64VB5 268 9,52838
64xnl 302 9,78284
666pP 268 9,61225
67hl9 268 9,67846
68sxJ 268 9,67859
69UAn 268 9,56016
6Wfmz 269 8,55445
6aWSk 293 9,63611
6anJj 293 9,71585
6bJv3 268 9,69084
6cBvU 293 9,72823
6cJF6 268 9,55094
6dpWV 287 9,30023
6duqQ 268 9,58260
6fK29 268 9,61225
6fQ84 268 9,64030
6ftOr 268 9,65229
6gHTg 268 9,57125
6gj8i 293 9,75176
6grck 268 9,53966
6h31q 302 9,70283
6hgNP 268 9,63662
6iTSx 268 9,59465
6iX5K 268 9,58279
6ioFC 268 9,57138
6jagd 268 9,58279
6kLXX 268 9,61238
6kbdO 293 9,70328
6kwtv 293 9,76524
6lMHl 268 9,64030
6lyHR 293 9,66408
6mVDp 268 9,48409
6nUwm 302 9,71476
6nwct 268 9,56003
6oH23 293 9,77433
6oV9s 268 9,61238
6okE7 268 9,71508
6pER1 268 9,69084
6pjkk 268 9,39860
6qxlN 268 9,69103
6rbVl 268 9,61238
6rvqY 268 9,62418
6sYwf 268 9,53966
6tK08 298 9,69148
6tMBh 268 9,61238
6tod1 268 9,64030
6uEuo 268 9,55101
6ueD3 268 9,69103
6v9nb 268 9,53966
6wArA 268 9,65209
6wu8Z 268 9,57222
7VHcr 268 9,55101
7X9p8 268 9,61490
7Y4Ox 268 9,61238
86sFP 268 9,63662
8eMK5 268 9,64030
8eV2K 268 9,53966
8kdVN 477 9,59813
8m1n3 268 9,65229
8pgZZ 293 9,65196
BAmE 268 9,49479
EF2u 268 9,69103
Orj9 268 9,69084
ykHl 268 9,69084
zaEa 302 9,48383
A0A8S5R9J3 268 9,60078
A0A8S5VUI1 268 9,69103
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_26828
3TOBT
 44 48,7% 283 2.604E-65
2 phalp2_26224
op4C
 2 37,7% 233 1.493E-45
3 phalp2_32070
5P7Lm
 156 35,0% 328 1.453E-38
4 phalp2_7826
aMK
 29 38,8% 211 4.318E-37
5 phalp2_11417
eYHn
 21 34,7% 273 5.944E-35
6 phalp2_14834
7s5S0
 47 33,9% 271 6.944E-34
7 phalp2_36039
5QA7W
 56 36,4% 222 1.058E-28
8 phalp2_14662
5ObrM
 161 36,5% 216 1.435E-28
9 phalp2_17183
3bJIf
 458 31,9% 272 1.151E-25
10 phalp2_2897
yopH
 100 25,8% 255 1.806E-09

Domains

Domains [InterPro]
Disordered region
Ami2
Unannotated
CW_7
Representative sequence (used for alignment): 5KvkK (314 AA)
Member sequence: A0A8S5Q394 (268 AA)
1 314 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF08230

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ctVif31
[NCBI] 		2825532 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015567 [NCBI]
CDS location
range 24888 -> 25694
strand +
CDS
ATGAATATCAATACCTCATTAATCAGCAACAACAACAGCTACGCAGGACAAACACCTCTGTATATTGTCATTCATAACACGGATAATATCGCCAAGACAGCAGATGCCAAAGCACACGCCACCGCACAGCATAATGGCAATTTTCATGGATATTCAGCCCATGTATTCGTTGACGATAAGTCAGCATACCAAGCATTGCCGTACAATCGCGGGGCATGGCATGTTGGAGTAAATTATGGCGGCAAACTGTTTGGGATAGTGAACAACCGTAATTCGATTGGAATTGAAATGTGCATGAATGCCGGCTATAACTACGAAAAGGCATTCCAAAATACCGTTGATGTATGTAAGCAGCTTATGAAGAAATATGGCATTCCAGCAAGCCGAGTAGTGCGGCACTACGATGTTTGCGCTAAGAATTGCCCTTCCGTTATCCGTGGAAAGGGAGACTGGAATAGATTTAAGAAGCTTATTTCCAGTGAAACCGTGACAGTTCCAACCACAAAGCCGACAGTAAAGGTTGACAAGTATTACCGTGTCCGCAAGACATGGAAGGATTCCAAGAGCCAGATCGGGGCTTATAAGTCCCTGGAAAATGCGAAAAAGGCTTGCAAAGCCGGTTACTCTGTTTTTGACTGGAATGGAAAAGCAGTGTATTCCGTGACTGCAAAGAAAAGTGTAGCCAAGGTAGCAAAAGAGGTAATCAACGGCGAATGGGGAAATGGACAGGACAGAAAAGACCGCCTGGAAGCTGCCGGGTACAATTACACAGAAGTGCAGAATGCAGTAAACAAACTTCTTAAATAA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (5KvkK) rather than this protein.
PDB ID
5KvkK
Method AlphaFoldv2
Resolution 82.68
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50