Protein

Protein accession
A0A8S5P687 [UniProt]
Representative
81mMl
Source
UniProt (cluster: phalp2_1605)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MNKINRMISNYNYNPGNISRIKYIVIHYVGALGGAQENCAYYGGGNRGASAHYFVGFAGEIWQCVEDRNIAWHCGASSYKHPECRNANSIGIEMCVRKKNAASLGATDKDWYFEGATVQSAIELTRYLMKKYNIPADHVIRHYDVTGKICPNPYVYNTGTYTWDAFKQAISGQSGDILPATDKPWYRVRKTWKNASSQIGAFKTMKKAKQCADQHTGYHVYNDAGKKVYTSAKLPYNVRPKTANVPIRTGAAKTYSAARTFLQPGKYVITEEKNGFGKLKSGAGWVYLKKVERV
Physico‐chemical
properties
protein length:294 AA
molecular weight:32951,1 Da
isoelectric point:9,63
hydropathy:-0,55
Representative Protein Details
Accession
81mMl
Protein name
81mMl
Sequence length
230 AA
Molecular weight
25826,68610 Da
Isoelectric point
8,75759
Sequence
MNINRKISKYNFDKGSVSRIKYIVIHYVGALGGAEDNCRYYGGGNRNASAHYFVGFNGEVWQCVEDANIAWHCGASSYKHAECRNANSIGIEMCVRKKNTKSMGETDKDWYFEDATVEAAAELTRYLMNKYGVPASHVIRHYDVTGKICPNPYVYNTSAHTWDEFKRKISRQAETPQGGNEKTIWNFLTGKGLNAYAVAGIMGNLYAESGLMPNNLQNNYNKKLGKTDAE
Other Proteins in cluster: phalp2_1605
Total (incl. this protein): 270 Avg length: 253,5 Avg pI: 6,95
Protein ID Length (AA) pI
81mMl 230 8,75759
10rbx 250 5,83364
1386y 338 9,03178
13CLf 263 8,88350
13bRd 244 5,45304
1EhmX 244 9,38036
1NAbU 222 6,45386
1bN5I 250 5,44145
1cBlO 228 5,07580
1gCIy 246 8,84682
1gCjL 211 9,39661
1jF0u 266 9,32608
1k2v8 228 5,07580
1keCA 283 5,45776
1knwp 259 9,44747
1m7OE 236 9,24917
1m8LY 287 5,49686
1mYZk 289 5,37477
1mch7 259 8,80337
1n3vr 278 8,50436
1n7tE 230 5,71354
1nbPd 233 5,58838
1neZ5 286 5,49601
21rKn 282 8,60268
23bqT 277 8,82735
24Hfg 254 8,03471
2HgNs 233 6,19252
2TMZf 231 5,90656
2TY85 231 5,90656
3ArAT 230 5,84688
3B05B 286 5,65937
3B1Sr 286 5,55984
3B1Sz 297 6,02047
3B2dF 230 5,91770
3BTev 205 6,58107
3GNO2 230 5,75747
3GQKg 230 5,79811
3IUKd 285 5,61492
3KPg6 287 5,77930
3KPiy 286 5,37620
3PF86 228 9,37649
3TC4C 223 8,89414
3VGXp 227 8,67037
3WMts 252 8,52886
3gODM 224 4,82349
3gOoo 248 6,17564
3gUXV 219 8,11942
3h6cS 241 8,97086
3ifFO 231 8,75521
3inBk 246 5,94885
3k5Q6 272 8,29529
3kuif 289 5,17919
3l7pk 284 5,26047
3lDog 272 8,66102
3lI3L 288 5,81414
3lYws 230 5,58332
3ljbS 231 5,90844
3loo5 282 5,24143
3m03d 286 5,36750
3maD0 260 8,72923
3mtW2 215 5,02453
3myk8 286 5,37620
3nQXp 239 5,99932
3oQkG 272 8,87009
3oZO7 246 9,65706
3pLEu 257 8,80614
3pSVU 286 5,29861
3pc1z 230 5,69825
3qFru 230 5,76151
3qcYF 230 5,67142
3qw7e 259 8,80337
3qwXn 278 8,76836
3qyVe 218 7,62378
3r4RB 288 5,69358
3r9vN 230 5,49499
3rVXO 230 5,84148
3rhjH 289 5,31481
3s9lB 292 5,64953
3sDbq 262 9,64765
3sHf5 223 7,60622
3sVYO 233 5,71774
3scr1 271 8,55084
3sgO4 238 5,75736
3sslt 231 5,48703
3t4i7 288 5,28724
3t7Me 281 8,27311
3tBgo 230 6,07509
3tIps 262 8,80337
3tbdl 230 5,60986
3tfFX 282 5,24143
3trm7 223 8,91806
3u9q6 233 5,59690
3uHnw 277 8,99303
3uXFf 230 5,85012
3uaTj 230 5,62759
3upEF 230 5,71081
3uuHA 235 5,34215
3vKNc 230 6,13591
3vLrv 288 5,99438
3vsd5 288 5,35971
3vvMK 289 5,38006
3w3jx 285 5,90036
3wn6j 289 5,38216
3x17C 289 5,38700
3xAzY 272 8,69732
3xBpb 226 8,86577
3xPND 233 6,85912
3xRT9 237 8,46865
3y1AA 230 5,98074
3y6Sy 230 5,84370
3yMOy 282 5,21392
3yVx8 228 5,69535
3yuK4 289 5,18215
3yyVL 230 5,48413
400bK 261 9,44618
40ZaJ 268 9,12165
41d7i 273 9,82719
45ApP 206 5,29156
45vPl 234 5,91401
4JYqT 363 9,44231
4JZYV 246 9,02327
4L5Ku 178 6,51280
4L76L 282 7,97759
4Lacj 238 5,73570
4SLmq 227 8,80956
4VITb 265 9,07214
4VsXc 261 9,37127
4kelH 205 8,39960
4kqGl 222 4,90500
4xqOA 315 8,80330
4y7Pa 270 9,37333
4z1on 280 5,29770
5BIY 285 8,29903
5BWp 251 9,33497
5K33L 243 9,44367
5L0J1 284 5,40535
5LoBR 254 9,57634
5OUNG 300 5,90969
5OUfp 282 5,51272
5Ohx7 282 5,10621
5P3X9 285 5,29770
5P7Mj 229 5,56757
5PpgW 284 5,74383
5QnEM 273 4,74756
5QqIG 282 5,57860
5RTjp 282 5,34960
5SZwU 258 5,03073
5SacY 285 5,27400
5SbVA 282 5,46560
5TCsE 276 9,56964
5TVq8 284 5,19113
5UAUB 282 5,20329
5UeCB 282 5,29145
5VkKF 283 5,37296
5W5ae 288 5,69728
5YoTX 254 9,57634
5Z6EN 213 8,42320
5ZEXA 275 9,56964
5ZTgs 228 5,17192
5hTSj 241 9,28108
5hVZL 241 9,22867
5jBk3 265 9,47732
60O8F 237 6,07617
60SXX 282 5,22262
61YKb 285 5,41166
63ODj 203 9,01772
63XEt 217 6,24958
63ve0 254 9,52799
64ff3 310 5,37176
66XjW 286 5,76730
687S 236 9,04512
69ehQ 205 6,05588
6PwCX 251 9,08580
6Z3PF 254 9,55359
6ZHFK 271 8,68462
6a07 303 5,93316
6a2AT 275 9,56964
6atet 285 5,40041
6b3M 268 5,13918
6c8cx 260 9,28894
6cGlG 201 8,18099
6ch1g 275 8,77461
6d1Tx 291 5,56536
6dBBX 285 5,19113
6djYL 235 5,29946
6drJb 243 5,99341
6hrKE 286 5,39558
6i7XO 254 9,55359
6iYP4 228 5,62259
6il7H 230 5,74923
6jvNx 230 5,62254
6kela 228 5,12974
6kqii 257 8,72826
6lhFl 221 8,75295
6mRst 283 5,31481
6muee 287 5,47333
6nwlI 283 5,47277
6oOTB 282 5,33061
6q69m 288 6,24498
6rZ6l 285 5,36983
6rjEs 282 5,42405
6sXx9 240 9,32208
6sgxl 288 5,40570
6tm4o 265 5,45048
6uPgi 275 9,58105
6w8Ao 286 5,70177
79FAU 236 6,08089
7DEs1 235 8,76269
7DPYo 241 8,31070
7NXTS 259 9,48454
7WIEZ 229 7,03595
7Xirw 233 6,36036
7Ygqv 227 6,23350
7Yox1 286 5,21710
7ZEf1 228 6,24191
7rYpA 262 9,19482
7yIfy 198 8,98433
7yd1e 252 6,45256
7ymVm 346 9,63140
80tua 235 8,24862
83w1H 216 9,16768
85fmy 227 6,39009
85uDt 228 4,87624
888Rx 260 8,57889
8aZ7x 228 4,81207
8bfuZ 229 9,31112
8cb65 205 7,00225
8f4LK 228 4,93120
8hPUl 235 6,19769
8kmDe 205 7,68085
8knHu 238 6,19638
8lv1p 243 6,31995
8nb7W 213 7,03857
8oRRb 284 5,39035
8pgUY 235 5,84063
8r5Jk 236 5,59957
8rMNc 282 5,27400
8rdH0 235 6,19928
8rynz 248 6,20417
8ryuu 205 6,58294
8s8sc 165 6,73607
8tcvg 285 5,27917
8vOq0 205 5,85381
DEbR 259 9,35863
F3EJ 287 5,76998
JSpi 285 5,19113
MUod 232 8,14044
NfAN 258 9,34123
OCl1 255 9,51903
UZ2m 233 5,43275
b4KM 225 6,74596
nAsy 187 6,22924
nbAj 246 6,35428
neY0 231 7,07011
nrW0 228 7,07540
nt7h 230 6,45113
ofJj 243 9,40138
ok0S 241 5,22506
omLW 236 9,09638
omUj 182 7,25717
on0l 216 9,33652
pOo1 275 9,47448
qJ6a 259 9,31744
qjXY 259 9,50465
rjn4 230 5,49328
wJZP 243 9,14634
wzRx 261 9,37127
xsY0 230 5,90628
yqiR 259 9,34987
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_40668
5i2H9
 1 52,5% 177 1.404E-70
2 phalp2_19131
1JgAd
 45 39,0% 197 2.155E-68
3 phalp2_29669
8sfID
 1005 48,8% 180 6.857E-67
4 phalp2_3793
5X3JD
 59 49,3% 160 8.576E-63
5 phalp2_7264
3WBGP
 11 44,8% 185 5.152E-55
6 phalp2_29517
21wOt
 7 39,8% 163 1.190E-53
7 phalp2_24299
3nIle
 36 49,7% 171 7.830E-53
8 phalp2_7116
2lzss
 1 40,3% 191 2.008E-52
9 phalp2_26828
3TOBT
 44 37,6% 191 1.807E-51
10 phalp2_31053
1GV4E
 13 43,7% 185 3.384E-51

Domains

Domains [InterPro]
Ami2
Phage_lys2
Representative sequence (used for alignment): 81mMl (230 AA)
Member sequence: A0A8S5P687 (294 AA)
1 230 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510, PF18013

Taxonomy

  Name Taxonomy ID Lineage
Phage Siphoviridae sp. ct58H1
[NCBI] 		2825334 No lineage information
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
BK015342 [NCBI]
CDS location
range 7449 -> 8333
strand +
CDS
ATGAACAAAATCAACCGCATGATCTCAAATTACAATTATAATCCCGGCAATATCTCCAGAATCAAATACATCGTGATCCACTACGTCGGAGCATTGGGCGGAGCACAGGAAAATTGTGCATACTATGGTGGTGGCAACCGTGGGGCATCTGCACACTATTTTGTCGGTTTCGCCGGCGAAATTTGGCAGTGCGTGGAAGATCGGAATATCGCCTGGCATTGTGGGGCGAGCAGCTATAAGCATCCAGAATGCAGAAATGCCAACAGTATCGGAATCGAGATGTGCGTGCGAAAGAAGAATGCGGCGAGTCTTGGGGCAACAGATAAGGACTGGTATTTTGAAGGAGCAACAGTACAGTCTGCTATTGAGCTGACCAGATACCTGATGAAAAAATATAACATCCCTGCAGATCATGTTATTCGCCACTATGATGTAACCGGAAAAATTTGCCCGAATCCGTATGTATACAATACAGGTACGTATACATGGGATGCCTTCAAACAAGCCATTTCTGGGCAGAGTGGCGATATTCTTCCAGCAACCGACAAACCATGGTACCGTGTCCGCAAGACCTGGAAGAATGCCAGCAGTCAGATCGGGGCATTTAAAACGATGAAGAAAGCCAAGCAGTGTGCAGATCAGCATACCGGTTATCATGTCTACAACGATGCCGGTAAAAAGGTGTACACATCCGCAAAACTCCCATACAATGTGCGGCCGAAAACCGCAAATGTTCCAATCAGGACAGGAGCGGCCAAAACATACAGTGCTGCCAGAACGTTTTTGCAGCCAGGTAAGTACGTGATCACCGAGGAAAAGAACGGATTCGGCAAGCTGAAAAGCGGTGCAGGATGGGTATATCTTAAGAAGGTAGAGAGGGTGTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (81mMl) rather than this protein.
PDB ID
81mMl
Method AlphaFoldv2
Resolution 91.97
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50